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The map label for this gene is yxeP [H]

Identifier: 218904797

GI number: 218904797

Start: 3516296

End: 3517441

Strand: Direct

Name: yxeP [H]

Synonym: BCAH820_3681

Alternate gene names: 218904797

Gene position: 3516296-3517441 (Clockwise)

Preceding gene: 218904796

Following gene: 218904802

Centisome position: 66.31

GC content: 39.79

Gene sequence:

>1146_bases
GTGGCAGCAAATCTAGAGCAATTAACTGAGACGCTAATTTCTATTCGTCGAAATTTACATGAGCATCCAGAACTATCATA
CGAAGAATTTGAAACGACGAAAGCTATAAAGAATTGGTTGGAAGAGGAAAACATTACGATTATAAATTCTAATTTAGAAA
CGGGCGTTATTGCTGAAATTTCGGGGAATCGTAACGGACCGCTTATAGCAATTCGTGCTGATATTGATGCCCTTCCTATT
CAAGAAGAAACAAATTTGCCGTACGCTTCAAAAATTCACGGTAAAATGCATGCTTGTGGGCATGATTTTCATACAGCTGC
TATTATAGGTGCCGCTTATTTATTAAAGGAAAAAGAGTCTTCTCTGAGCGGAACTGTACGCTTTATATTCCAGCCAGCTG
AAGAAAGTAGCAACGGTGCTTGCAAAGTCATTGAAGCTGGGCATTTACATGGCGTGCAAGCTATTTTCGGTATGCATAAT
AAACCTGATTTACCAGTAGGTACAATCGGTATTAAAGATGGTCCACTGATGGCCGGAGTTGATCGATTTGAAATTGAAAT
TCACGGAGTTGGTACACATGCAGCTGTACCAGACGCTGGGGTAGATCCTATTGTCGCGTCCTCTCAAATTGTTATGGCAC
TCCAAACAATTGTAAGCCGAAATATAAGTTCCTCTCATAATGCCGTCGTTAGTGTCACAAACATACATTCAGGAAATACG
TGGAATGTCATTCCTGAAAAAGCTACGTTGGAAGGAACTGTACGTACATTCCAAACGGAAACACGTGAAAAGATTCCTGC
ACTCATGAAACGTATTATTCAAGGAGTTTCTGATGCGTTAGGTGTAAAAACAGAATTTCGTTTTTATGCAGGTCCTCCTG
CCGTTCATAATGATACCTCTCTAACAAACTTATCGACTCAAGTTGCAGAAACAATGAATTTAAATATCATCTCACCTACC
CCTTCAATGGCCGGAGAAGATTTTTCTTTTTATCAACAAGAAATACCTGGTTCATTCGTCTTTATGGGAACGAGTGGTAC
ACACGAATGGCATCATCCAGCTTTTACATTAGACGAGCGAGCATTGCCCATAAGTGCAGCATACTTCGCTTTATTAGCTG
AAAAAGCACTTAAACACTTTTCATAA

Upstream 100 bases:

>100_bases
ATTGGCAGCATAAACGAGAGAGGATTTTCATTTGAGAATCCTCTCTTTTAATACTGTATACTTAATGTAAATACTTTATT
TAAGAAAGAAGGCATTATAA

Downstream 100 bases:

>100_bases
AAAAGGAGCCGTTAAGAAATGCTTAACGGCTCCTTTTTTTAAGTTAAATCACGTTTTAAAAATACTGTATAAGAAATGCT
TAAAAATACAAATAAATACA

Product: thermostable carboxypeptidase 1

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAANLEQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEISGNRNGPLIAIRADIDALPI
QEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHN
KPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNT
WNVIPEKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIISPT
PSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDERALPISAAYFALLAEKALKHFS

Sequences:

>Translated_381_residues
MAANLEQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEISGNRNGPLIAIRADIDALPI
QEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHN
KPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNT
WNVIPEKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIISPT
PSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDERALPISAAYFALLAEKALKHFS
>Mature_380_residues
AANLEQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEISGNRNGPLIAIRADIDALPIQ
EETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNK
PDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTW
NVIPEKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIISPTP
SMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDERALPISAAYFALLAEKALKHFS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=393, Percent_Identity=29.7709923664122, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 41433; Mature: 41302

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLEQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEI
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEE
SGNRNGPLIAIRADIDALPIQEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKES
CCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCC
SLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGV
CCCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEECC
DRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNT
EEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCE
WNVIPEKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
LTNLSTQVAETMNLNIISPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDER
HHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCC
ALPISAAYFALLAEKALKHFS
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AANLEQLTETLISIRRNLHEHPELSYEEFETTKAIKNWLEEENITIINSNLETGVIAEI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEE
SGNRNGPLIAIRADIDALPIQEETNLPYASKIHGKMHACGHDFHTAAIIGAAYLLKEKES
CCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCC
SLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGV
CCCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEECC
DRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNT
EEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCE
WNVIPEKATLEGTVRTFQTETREKIPALMKRIIQGVSDALGVKTEFRFYAGPPAVHNDTS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
LTNLSTQVAETMNLNIISPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTLDER
HHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCC
ALPISAAYFALLAEKALKHFS
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]