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The map label for this gene is kinE [H]

Identifier: 218904259

GI number: 218904259

Start: 2974307

End: 2976712

Strand: Reverse

Name: kinE [H]

Synonym: BCAH820_3143

Alternate gene names: 218904259

Gene position: 2976712-2974307 (Counterclockwise)

Preceding gene: 218904260

Following gene: 218904258

Centisome position: 56.14

GC content: 32.21

Gene sequence:

>2406_bases
ATGATTATTAAAGATTATTTCATCAATCTTTCTATTTTTTCTTTATTAGTTAGCGCAGCAATATTTATTCAAGTGTTTAC
AATTAATTCTAGTCGATATTTCGAAAAACTCTATGGAGGGATTATTGCAGTTACGTTAATGTTCTTTTCTTTTCCATACA
TGGGATTTTCCTACGATCTTCGAGTTGTTCCTCTTATTCTATCTTTTATTTACTTTGGTCGTATTGCTGGTTGGATTACG
TTAATTAGCATAATTTTAATGCGTGTCTTTTACATTGGAGGGTATTGGGAACCTCCTGTGCTTGCTTATTTAGGTATAAG
TATCCTGTTTGTTAGTTTTAAAACATATTTTAAAAACCTTCATCCTTTTAAAAGTGCATCTTTATATTTTTCTGTTTTTG
TCGGAATAAAGTGGTTAGTTGGCGTATTATTTAATACTACATTACTTTACACAGGAGGCTTATTATATATAGCGTTAGGA
CTTTTAATTGGGCTATTTCTTATGGAAGCCTACCAGAGATTGTATTATTTAACAAAGGACTTATCTAAAATGAATCGAGA
ATTAAAAAGATCGAAGCAAGAACTTACAGATACCGTACATGAGCTTCAAGGAGGAATTTTTAAATTTAAAAAAGTGGGTA
AGCACTTTATACACACTTTATGTGATGGACAGTTTTATTATCAAAACGGATTTTATTCTGAACAGGTGGTAGGAAAAAGC
TTACGTACTATTGATGCTTCTATTATTCCTCCACATTTAGTTCCACAATTGATGAAGTACTATCTTCAGGCATGGGAAGG
AAAAGAAATTATATTTGAATTACCTTGGCCAAATGATAAAACTATTATTCTTATTGCACTTAGGCCAATTAAACGAAATG
GACAAGTTATTGAAGTTGTTGGTTCTACAGTTGATATAACTGAAAGGAAAAAGGTAGAATCAGAATTAAGCGCTACCAAA
GAATTATTAGAATCATTTATAAAGCACAATCTAGATGCTATTACCATTTCTGATCGAGAAGGGTATATTTTACAAGCGAA
TAAAGCTTATGAAAAGATATTTGGTTGGTCATCACAAGAAATCATAGGTAAGAGATTACCTTGTGTACCAGATTTTTTAA
TGGAAGAATCACTTAAAAATATTCAGAAAATTCTAACAGGTGAATCTGTGGTTACTAGATTAGAAACTGTTAGACAACGT
AACGATGGGAGTCTTCTTGATGTCAGTCTGACAGTTTCTCCTATATTAGATGTAAGAGGAAATGTGATAGCTTTATCAGC
AATATGTAGAGACATCTCTGAAAGGAAACAAGCAGAAAGAGAACGGCATCGATTACACCAGCAATTAAGAGATAGTGAAA
TGAAATACCGTGCACTAATCGAACAAGCAACTGATGCCGTATATGTAGTAGAGCTGAATGAAGAGAATGCTCCAAGTCGA
TTTATTGAGGTAAACCCTGTTGGGTGTAGAAGATTTGGATATAGTAGAGAAGAGTTGCTCTCATTATCATTTTCAAATAT
AGTACCACAAGATTCTCAAATGATCGTAAGGTTATTAGAAAAAATTAAAAAGGGACAAACTTCCTTCACTTTGCAAGATG
AATATGTTTTTCCAACAGGAAAAGTAATAACAACTGAGTTTAGTGTTCGTGTTTTTAACTTAAATGGTAAAAAAGTTTTC
CTAAGTATTTCTCGTGATATCACTGAACGGCTAAAAACAGAAGAATTATTGCGAAAATCTGAAAAACTTGCTGTTGTAGG
ACAATTAGCGACTGTAATGGCTCATGAGATTAATAATCCATTAACTGCAATGAAAGGGTTCATGCAATTACTAAAATCAA
CGGAAAATGAGAATAATCAGGGTTATATTAATATAGTATCATCAGAGATTGAGCGTATAGAGAGCATTACAACTGAATTT
ATGGCGGTAGCCAAACCACAGGTGGTAAAAATACAACCTAATGATATTAGTGTGCTAATGGATCAAGTTTTAATGCTACT
ACAACCTCAAGCAATGATGAATAATATAAAATTTAGAATCGATCTTAACCCTGGTATTCCATTGATTTCATGTGAAGGAA
ATCAATTAAAACAAGTATTTGTTAATATATTAAAAAATGCAATTGAATCTATGCCGATGGGAGGGGAAATTCTCATTCAA
TTAAATATACTTGATAATAATCAAGTAAGCATTCGTTTTATCGATCAAGGGTGTGGGATTCCAAAAGAACGTATACCATA
TCTCGGAGAACCTTTTTATAGTATTAAAGAAGAGGGGATTGGCTTAGGGTTAATGATCTGTTATAAAATCATTGAAACAC
ATCAGGGAAAGATATTTATTGAGAGTGAAGTGAATAAGGGAACTACAGTTGAAGTCACTCTTCCTATTTTTACACTTCAA
AATTAA

Upstream 100 bases:

>100_bases
TTATTTACTTTTCTAGATTTTATGCATCTGAACTGTTTATACTATTTGTAGGATTTTTAGCCTTTTTGTCGAATACACTT
AATGCAAGGAGGGACATTCC

Downstream 100 bases:

>100_bases
TATATTCTTTTACTGAAATTTTTTAAGAAACCATACAGAATGTAATGCTAATGTTACTTAATGAAATAGTAAAGAGAAGA
ACACTGGTAAATGTACCCTA

Product: sporulation kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MIIKDYFINLSIFSLLVSAAIFIQVFTINSSRYFEKLYGGIIAVTLMFFSFPYMGFSYDLRVVPLILSFIYFGRIAGWIT
LISIILMRVFYIGGYWEPPVLAYLGISILFVSFKTYFKNLHPFKSASLYFSVFVGIKWLVGVLFNTTLLYTGGLLYIALG
LLIGLFLMEAYQRLYYLTKDLSKMNRELKRSKQELTDTVHELQGGIFKFKKVGKHFIHTLCDGQFYYQNGFYSEQVVGKS
LRTIDASIIPPHLVPQLMKYYLQAWEGKEIIFELPWPNDKTIILIALRPIKRNGQVIEVVGSTVDITERKKVESELSATK
ELLESFIKHNLDAITISDREGYILQANKAYEKIFGWSSQEIIGKRLPCVPDFLMEESLKNIQKILTGESVVTRLETVRQR
NDGSLLDVSLTVSPILDVRGNVIALSAICRDISERKQAERERHRLHQQLRDSEMKYRALIEQATDAVYVVELNEENAPSR
FIEVNPVGCRRFGYSREELLSLSFSNIVPQDSQMIVRLLEKIKKGQTSFTLQDEYVFPTGKVITTEFSVRVFNLNGKKVF
LSISRDITERLKTEELLRKSEKLAVVGQLATVMAHEINNPLTAMKGFMQLLKSTENENNQGYINIVSSEIERIESITTEF
MAVAKPQVVKIQPNDISVLMDQVLMLLQPQAMMNNIKFRIDLNPGIPLISCEGNQLKQVFVNILKNAIESMPMGGEILIQ
LNILDNNQVSIRFIDQGCGIPKERIPYLGEPFYSIKEEGIGLGLMICYKIIETHQGKIFIESEVNKGTTVEVTLPIFTLQ
N

Sequences:

>Translated_801_residues
MIIKDYFINLSIFSLLVSAAIFIQVFTINSSRYFEKLYGGIIAVTLMFFSFPYMGFSYDLRVVPLILSFIYFGRIAGWIT
LISIILMRVFYIGGYWEPPVLAYLGISILFVSFKTYFKNLHPFKSASLYFSVFVGIKWLVGVLFNTTLLYTGGLLYIALG
LLIGLFLMEAYQRLYYLTKDLSKMNRELKRSKQELTDTVHELQGGIFKFKKVGKHFIHTLCDGQFYYQNGFYSEQVVGKS
LRTIDASIIPPHLVPQLMKYYLQAWEGKEIIFELPWPNDKTIILIALRPIKRNGQVIEVVGSTVDITERKKVESELSATK
ELLESFIKHNLDAITISDREGYILQANKAYEKIFGWSSQEIIGKRLPCVPDFLMEESLKNIQKILTGESVVTRLETVRQR
NDGSLLDVSLTVSPILDVRGNVIALSAICRDISERKQAERERHRLHQQLRDSEMKYRALIEQATDAVYVVELNEENAPSR
FIEVNPVGCRRFGYSREELLSLSFSNIVPQDSQMIVRLLEKIKKGQTSFTLQDEYVFPTGKVITTEFSVRVFNLNGKKVF
LSISRDITERLKTEELLRKSEKLAVVGQLATVMAHEINNPLTAMKGFMQLLKSTENENNQGYINIVSSEIERIESITTEF
MAVAKPQVVKIQPNDISVLMDQVLMLLQPQAMMNNIKFRIDLNPGIPLISCEGNQLKQVFVNILKNAIESMPMGGEILIQ
LNILDNNQVSIRFIDQGCGIPKERIPYLGEPFYSIKEEGIGLGLMICYKIIETHQGKIFIESEVNKGTTVEVTLPIFTLQ
N
>Mature_801_residues
MIIKDYFINLSIFSLLVSAAIFIQVFTINSSRYFEKLYGGIIAVTLMFFSFPYMGFSYDLRVVPLILSFIYFGRIAGWIT
LISIILMRVFYIGGYWEPPVLAYLGISILFVSFKTYFKNLHPFKSASLYFSVFVGIKWLVGVLFNTTLLYTGGLLYIALG
LLIGLFLMEAYQRLYYLTKDLSKMNRELKRSKQELTDTVHELQGGIFKFKKVGKHFIHTLCDGQFYYQNGFYSEQVVGKS
LRTIDASIIPPHLVPQLMKYYLQAWEGKEIIFELPWPNDKTIILIALRPIKRNGQVIEVVGSTVDITERKKVESELSATK
ELLESFIKHNLDAITISDREGYILQANKAYEKIFGWSSQEIIGKRLPCVPDFLMEESLKNIQKILTGESVVTRLETVRQR
NDGSLLDVSLTVSPILDVRGNVIALSAICRDISERKQAERERHRLHQQLRDSEMKYRALIEQATDAVYVVELNEENAPSR
FIEVNPVGCRRFGYSREELLSLSFSNIVPQDSQMIVRLLEKIKKGQTSFTLQDEYVFPTGKVITTEFSVRVFNLNGKKVF
LSISRDITERLKTEELLRKSEKLAVVGQLATVMAHEINNPLTAMKGFMQLLKSTENENNQGYINIVSSEIERIESITTEF
MAVAKPQVVKIQPNDISVLMDQVLMLLQPQAMMNNIKFRIDLNPGIPLISCEGNQLKQVFVNILKNAIESMPMGGEILIQ
LNILDNNQVSIRFIDQGCGIPKERIPYLGEPFYSIKEEGIGLGLMICYKIIETHQGKIFIESEVNKGTTVEVTLPIFTLQ
N

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=30.4347826086957, Blast_Score=166, Evalue=6e-42,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=28.4518828451883, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI48994928, Length=458, Percent_Identity=22.2707423580786, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790300, Length=324, Percent_Identity=25.6172839506173, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=28.4090909090909, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=282, Percent_Identity=22.3404255319149, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=27.0161290322581, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=24.2290748898678, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1788393, Length=214, Percent_Identity=21.9626168224299, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 91483; Mature: 91483

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCCEEEEEEEEEEECC
>Mature Secondary Structure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EECCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]