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The map label for this gene is dnaN2 [H]

Identifier: 218903815

GI number: 218903815

Start: 2567398

End: 2568528

Strand: Direct

Name: dnaN2 [H]

Synonym: BCAH820_2699

Alternate gene names: 218903815

Gene position: 2567398-2568528 (Clockwise)

Preceding gene: 218903814

Following gene: 218903816

Centisome position: 48.42

GC content: 32.1

Gene sequence:

>1131_bases
ATGGAGTTTATCGTTAATCATAAACATTTCACTCAAGCACTTTCAGAAGTAAGTAAAGCAATATCTACGAAAGCTGTAAT
TCCTATACTATCCGGTATAAAAATAACTGCAGATCAATCTGGAATTACTTTAATCGCAAGTAATTCGAATATTTTTATCG
AAAAATTTATACCTAGTGCTATAGATGACGAACAAATTACGACTATCTTACAAGTAGGAACAATTGTTGTACCTGCAAAA
TACTTCATTGAAATTATAAAGAAAATGCCAAGCGATATAGTAATAAAAAGTAAGAATGAGCAAACAATTACCATACAATC
AGGAGAAATCACATTAAACTTAAATGGCTTTCCAGTGAATGAATTTCCTAATGTACCACAAATAGACGATCATACAGAAA
TACAAATAGAAACAAAACAATTGATTGATGCGTTTAAACAGACAGTCTTTGCAGTAGCGAAAAATGAATCTAGACCCGTT
CTTACTGGCGTACATATCGAGTTAGATCATAACAAATTAATATGCGCTGCAACTGACTCTCACAGACTAGCTATACGTGA
AACACTAATTTCTACTAATATGAAAGCGAATTGTATTGTACCAAGTGCAACCATTAATGAGCTTTTAAAATTAATGAACA
GCAATTTAGAATTCGTTTCTATTTATCTATCAGAAAGTCACATTATTTTTACATTCGGCACAACTACGTTATATTCACGA
CTAATTGAAGGGAAATATCCTAATATTTCTACTCTCATTCCAAATGAATTTCAAACCGTCATTAACATAGATAGACAAAG
AATGTTACAAGGGGTAGATCGATCAAGCTTATTAGCGAGTGAATGGGCAAATAACAATGTTAACTTAGAAATTGTAAACG
AATCTACAATTCAAATTTCTTCTAACGCTTCTCAGATCGGAAAAATATCTGAGACGCAACAGATAGATGTAATTCAAGGT
GAAAAACAATTAAATATATCTTTCGATGGACGATTTATGTTGGATGCTTTAAGGGCAATAAAAGAAGAAACGGTTACTTT
AAGTTTCAGCGGTTCTATGAGACCAATATTAATTGAAGCAGGGACACAATCTGCAGCAATCCATCTTATATCTCCAGTAA
GAGCTTATTAA

Upstream 100 bases:

>100_bases
AAATATATTACAAAGGAAAGAAACAGTACCTTACTTTTTTAACGGAACAATCATTCATCAATCTTCATAAAACTATCATT
TCATGTAAGGTGGGGAAAAA

Downstream 100 bases:

>100_bases
AAACAGAGGAAGGAATCACATTATGTACAAACACACTTTATGTTTCATAAAAAGAAATGAAGAGATACTTATGCTAAATA
GAAAATATGACCCTGTAAAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MEFIVNHKHFTQALSEVSKAISTKAVIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQVGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPVNEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
EKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Sequences:

>Translated_376_residues
MEFIVNHKHFTQALSEVSKAISTKAVIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQVGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPVNEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
EKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY
>Mature_376_residues
MEFIVNHKHFTQALSEVSKAISTKAVIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQVGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPVNEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
EKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=385, Percent_Identity=23.6363636363636, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41695; Mature: 41695

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIVNHKHFTQALSEVSKAISTKAVIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQVGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPVN
CCHHHHHHHHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCEECC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGEKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MEFIVNHKHFTQALSEVSKAISTKAVIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQVGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPVN
CCHHHHHHHHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCEECC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGEKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]