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The map label for this gene is thiF [H]

Identifier: 218903283

GI number: 218903283

Start: 2052936

End: 2053949

Strand: Direct

Name: thiF [H]

Synonym: BCAH820_2167

Alternate gene names: 218903283

Gene position: 2052936-2053949 (Clockwise)

Preceding gene: 218903282

Following gene: 218903284

Centisome position: 38.72

GC content: 38.07

Gene sequence:

>1014_bases
ATGCAAGAGCGATATTCAAGACAAGTATTGTTTTCTGGAATAGGTGAAATGGGACAAAGGAAAATAAGGGAAAAGCATGT
GCTCTTAATTGGTGCGGGGGCGCTAGGAGCTGCAAATGCAGAAGCGCTCGTTCGGATGGGAATTGGGAAATTGACAATTG
CCGATCGTGACTATGTCGAATGGAGTAATTTACAACGGCAACAGTTATATACAGAAGAAGATGCAAAACAATGCAAACCA
AAAGCAATTGCAGCGGCAGAACATGTAAGAAAGATTAATTCTGAGGTGGAAATTGTACCAGTTGTAACGGATGTCACAAT
GAAAGAAATGGAAGAGTTAACGAAAGAAGTGGATCTCATATTAGATGCGACTGACAACTTTGATACGCGTCTACTTATAA
ATGATATTTCACAAAAAGAAAATATACCTTGGATATACGGTGGATGCATTGGAAGTTACGGTGTAACGTACACAATTCTT
CCAGGAAAAACACCATGTTTTCGCTGCTTAATGGATCATCCGATGGGCGGTGCAACATGTGATACAGCGGGAATTATTCA
GCCAGCTGTACAGATGGTTGTTGCTCACCAAGTGGCAGAAGCAATGAAAATATTGGTGGATGATTTCGAGACGCTACGAG
GAACAATGTTATCATTTGATATTTGGAACAATCAATTTCTCTCATTAAAAGTAAATAAACAGAAAAAAAGTACATGTCCA
TCTTGCGGAAATACACGTACGTACCCAAGTTTAACATTTGAATCACAGGTGAAAACGGAGGTGCTATGCGGACGGAATAC
AGTTCAAATCCGTCCAGGAATAAAGAGAGTTCTATATTTAAACGAAATCAAAAAAAGATTACAAAAAATTGTACATGTCC
AAAAAACGCCGTACTTACTATCATTTCTAATTGATGAATATCGTTTCGTTTTATTTACAGACGGTAGGGCATTTATTCAT
GGGACAAATGATGTGAAAATAGCAAAACGATTGTACGCAAAATATATAGGATGA

Upstream 100 bases:

>100_bases
GAACAGATCGATATTCAGATGAAAGAACGGCGGAAAGTACAAATAAACGTCAAAAAGTTGAGATGTCTTACATTGGTGGA
TAGCGAAAGGAGGATTCCTT

Downstream 100 bases:

>100_bases
ACAGAGAGAATTTCCCCCTTATTAAAACGAGGTGAAAAAGAATGTTAGCAAAACGTATACCGATTCCAGTGGCAGAAGCA
GTTGCACGAGTAATGAAATA

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVAEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLYLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFIH
GTNDVKIAKRLYAKYIG

Sequences:

>Translated_337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVAEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLYLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFIH
GTNDVKIAKRLYAKYIG
>Mature_337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVAEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLYLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFIH
GTNDVKIAKRLYAKYIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=267, Percent_Identity=30.3370786516854, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI38045948, Length=170, Percent_Identity=32.3529411764706, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=183, Percent_Identity=29.5081967213115, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787048, Length=242, Percent_Identity=32.6446280991736, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=34.9397590361446, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17540406, Length=265, Percent_Identity=32.0754716981132, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193203301, Length=162, Percent_Identity=30.2469135802469, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=270, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=35.5828220858896, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325323, Length=155, Percent_Identity=32.9032258064516, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325438, Length=153, Percent_Identity=30.718954248366, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=264, Percent_Identity=28.030303030303, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28573937, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24641311, Length=163, Percent_Identity=33.7423312883436, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 38045; Mature: 38045

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVE
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQCKPKAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHEE
LDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTILPGKTPCFRCLMDHPMGGATC
EECCCCCCCEEEEECHHHCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQMVVAHQVAEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCCCCC
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLYLNEIKKRLQKIVHVQKTPYLL
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
SFLIDEYRFVLFTDGRAFIHGTNDVKIAKRLYAKYIG
HHHHHCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVE
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQCKPKAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHEE
LDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTILPGKTPCFRCLMDHPMGGATC
EECCCCCCCEEEEECHHHCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQMVVAHQVAEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCCCCCCCC
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLYLNEIKKRLQKIVHVQKTPYLL
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
SFLIDEYRFVLFTDGRAFIHGTNDVKIAKRLYAKYIG
HHHHHCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]