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The map label for this gene is rsmA [H]

Identifier: 218884716

GI number: 218884716

Start: 1306871

End: 1307707

Strand: Reverse

Name: rsmA [H]

Synonym: DKAM_1405

Alternate gene names: 218884716

Gene position: 1307707-1306871 (Counterclockwise)

Preceding gene: 218884717

Following gene: 218884715

Centisome position: 95.79

GC content: 43.25

Gene sequence:

>837_bases
ATGGGGAGCTATATTAGGCCAGATGAAATGAGTAGACGCGGCCTCCTTTCATGGACTATAAGCATACTCCGCGAGCATGG
TTTAAGACCCAGGAGGAAGCTTAGTCAGAACTTCATCGTAGATCCCCGGCTTATAAGGGACTTCATAAGCCATGTAAACC
ATGCGGAAACCACTGAAATAGGCTGTGGACTAGGTACTTTAACCATTCCATTAAGCAGAGTGGTTCCTAGACTTATATGC
ATAGAGATCGATGAGAAACTACTGGGTATAGCTGTTAAAAACTTGAATACATCTAACACTCTCTTCATCAATGCTGATGC
CACCAAGTACACGGTGTTCTCTAGGCAAGTGGTAGGGAATATACCATACCATGTTACATCCAATATATTGGTTTCAATAG
CTAGATCGAATAACGTTGAAAGAGTTGTATTTACGCTTCAAAAAGATGTCGCTGAGAGACTTGTAGCCAAGCCAGGTAGT
AAGCAGTATGGTAGGATAACTATTCTACTCAACACATTGTTTAACATAGAAATCACAGGTATATATGGCCCCTCCTCATT
CTACCCTGAACCAAAGGTAGGGCACGCAATTATAACTTTAACCAGGCGACGCGTTTTTAACCAGGATGTATTCGCCCTCG
AGAAGCTGACCAGGCTATTGTTTACGCAGAGGAGAAGGATAGCTTTAAGGGTTCTCACCAAGTCCCTTGGTATAGGGGAG
GACAGCGAGATATATATGTATGCCCGTGAACTACTTGGAGATAAGAGAGTATATGAGGTAAGCGGAGAGGTGTATGCGAC
ATTAGCGAGAAGACTAAGGGAGAATGGATTACTGTAG

Upstream 100 bases:

>100_bases
AACAGGGTTATCAAGGAGTTGATTGGTGGGGAGAAGTACTATTTATTCATAAAATCCCCCGAGCCTAACACGCTGTACCT
AGGGTACTTGGAGAAATTAT

Downstream 100 bases:

>100_bases
GGAGTCTCAAACTAGTATTTAAGGGAAACGTGTACAGGCCTAGCGATGACTCATGGCTCGTAGTAAAGTTACTGGACTCC
ATTAAACCGAGAGCGGATCT

Product: putative dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MGSYIRPDEMSRRGLLSWTISILREHGLRPRRKLSQNFIVDPRLIRDFISHVNHAETTEIGCGLGTLTIPLSRVVPRLIC
IEIDEKLLGIAVKNLNTSNTLFINADATKYTVFSRQVVGNIPYHVTSNILVSIARSNNVERVVFTLQKDVAERLVAKPGS
KQYGRITILLNTLFNIEITGIYGPSSFYPEPKVGHAIITLTRRRVFNQDVFALEKLTRLLFTQRRRIALRVLTKSLGIGE
DSEIYMYARELLGDKRVYEVSGEVYATLARRLRENGLL

Sequences:

>Translated_278_residues
MGSYIRPDEMSRRGLLSWTISILREHGLRPRRKLSQNFIVDPRLIRDFISHVNHAETTEIGCGLGTLTIPLSRVVPRLIC
IEIDEKLLGIAVKNLNTSNTLFINADATKYTVFSRQVVGNIPYHVTSNILVSIARSNNVERVVFTLQKDVAERLVAKPGS
KQYGRITILLNTLFNIEITGIYGPSSFYPEPKVGHAIITLTRRRVFNQDVFALEKLTRLLFTQRRRIALRVLTKSLGIGE
DSEIYMYARELLGDKRVYEVSGEVYATLARRLRENGLL
>Mature_277_residues
GSYIRPDEMSRRGLLSWTISILREHGLRPRRKLSQNFIVDPRLIRDFISHVNHAETTEIGCGLGTLTIPLSRVVPRLICI
EIDEKLLGIAVKNLNTSNTLFINADATKYTVFSRQVVGNIPYHVTSNILVSIARSNNVERVVFTLQKDVAERLVAKPGSK
QYGRITILLNTLFNIEITGIYGPSSFYPEPKVGHAIITLTRRRVFNQDVFALEKLTRLLFTQRRRIALRVLTKSLGIGED
SEIYMYARELLGDKRVYEVSGEVYATLARRLRENGLL

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786236, Length=273, Percent_Identity=27.8388278388278, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25146882, Length=193, Percent_Identity=28.4974093264249, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358017, Length=187, Percent_Identity=25.1336898395722, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31612; Mature: 31481

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSYIRPDEMSRRGLLSWTISILREHGLRPRRKLSQNFIVDPRLIRDFISHVNHAETTEI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
GCGLGTLTIPLSRVVPRLICIEIDEKLLGIAVKNLNTSNTLFINADATKYTVFSRQVVGN
CCCCCHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHCC
IPYHVTSNILVSIARSNNVERVVFTLQKDVAERLVAKPGSKQYGRITILLNTLFNIEITG
CCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEE
IYGPSSFYPEPKVGHAIITLTRRRVFNQDVFALEKLTRLLFTQRRRIALRVLTKSLGIGE
EECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSEIYMYARELLGDKRVYEVSGEVYATLARRLRENGLL
CCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSYIRPDEMSRRGLLSWTISILREHGLRPRRKLSQNFIVDPRLIRDFISHVNHAETTEI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
GCGLGTLTIPLSRVVPRLICIEIDEKLLGIAVKNLNTSNTLFINADATKYTVFSRQVVGN
CCCCCHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHCC
IPYHVTSNILVSIARSNNVERVVFTLQKDVAERLVAKPGSKQYGRITILLNTLFNIEITG
CCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEE
IYGPSSFYPEPKVGHAIITLTRRRVFNQDVFALEKLTRLLFTQRRRIALRVLTKSLGIGE
EECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSEIYMYARELLGDKRVYEVSGEVYATLARRLRENGLL
CCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]