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The map label for this gene is hisS [H]

Identifier: 218884522

GI number: 218884522

Start: 1132761

End: 1134083

Strand: Reverse

Name: hisS [H]

Synonym: DKAM_1211

Alternate gene names: 218884522

Gene position: 1134083-1132761 (Counterclockwise)

Preceding gene: 218884523

Following gene: 218884521

Centisome position: 83.07

GC content: 48.22

Gene sequence:

>1323_bases
GTGAGTGAGGAAGACGTATTGAACCCCCCGAGGGGGATGAGGGATCTAACAGGTGTCGATGCTGAGTTACACGAGTACAT
GGTTGAATTATTTAAGGAGACGGCTAGGCGAAACGGGTTCAAGCCTATTATAACGCCTACAGTAGAATACTTTAGGTTAT
TCGAGAAGAAGAGCGGCGAAGAGATAAAGAGGTCTATGTATGTCTTCCAGGATAAAGCCGGTAGAACACTCGCGTTAAGA
CCCGAGGTCACTGCAAGCGTTGTTAGAGCGTATCTTAAGGAGATGAGGTCTCAGCCTAAGCCCATTAGGCTATACTATGT
AGCCCAGTGCTTTAGATACGAGGAGCCGCAGAAAGCGAGGTACAGGGAGTTCTGGCAGGGGGGATTAGAGATCATAGGCG
ACCCCGATATAAATGCTGATGTCTCAGCTGCTTTCACAGCTAGCAACTTCCTTGAAGAGCTTGGTGTGAAACACTACTAT
GTAGTAGGTAATGTCGCAACCTACAGGGTCATCATGGATAGAATAGGTATCCCGGAGGAAACACAGGACCTAGTGCTACA
CTTAATCGATAAGGACAGGGTTGGCGAAGCCCTTCAGGTGCTCAGGGATAAGACTAGCAGGGAGGATATCGTTGAATTAT
TCGGGAGGCTCCTGAATAGCGGGATAGATGAAGCATTAAGCGTTATAATGGACTTCAAGGGGCTGGGCGAGGGATACTCT
ATCATAGAATCCGAGCTGGCTAAGACAAGGGAGTTCATAGAGTTGTTGAATCAGGTTGGATTTAACGCTGCATACGAGCC
TAAATTGGTTAGGGGGCTGGCCTACTACACCGGGTTGATCTTCGAGTATAAGACAAGTGGTGGATTAAGTGTGAGCATTG
GTGGGGGCGGTAGATACGATGGATTAACCAGGGTGTACGGGGGAGAATACGAGTATTCTACCGGTTTAGCACTGGGACTG
GATAGAATTATGCTGCTACTGGACCCCGGTAAGATACTTCCCAGTAAAACCACCGTGCTAATCTCAATACTAGAGGGTGT
TCCACTTAAAGCCGGGTATGAACTAGTGAGGCGAATCAGGGTTAAAGGGGTTGAAGTAATCCCGTATAGGGCGCGCTCGT
TATCCAAGGCGCTGAGCCTGGCGAACAAGATTAACGCTGGATTAGTAGTGATAATTGGTAGGAGAGAGTATGAGTCAGGC
TCTATAACAGTAAAGAATATGATCACGGGTGAGCAGAAATACATAGGGTTAAACGACATCAATGCATTCTTGGACTACAT
AAAGGGCGCCGGGGCGCAGCAGCCCCAGGATGATGCGGGCTAG

Upstream 100 bases:

>100_bases
TAGAAGCAATAGTCTTCAACTAGTTCTAGCAATAGTTAGCCTCCAGGGGGATATAACATACTATGAGGCAAACAAGATAG
CGTTGGAGGTGGTTTAAACA

Downstream 100 bases:

>100_bases
ACAATGTTCTACCCGTGTTTATTAAAACCCATTTACATAAACCATAATTATAGAGTTGTAAAAAGGTGCATTTCTATGAC
CAGGATAAGTCTTGAGAAAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSEEDVLNPPRGMRDLTGVDAELHEYMVELFKETARRNGFKPIITPTVEYFRLFEKKSGEEIKRSMYVFQDKAGRTLALR
PEVTASVVRAYLKEMRSQPKPIRLYYVAQCFRYEEPQKARYREFWQGGLEIIGDPDINADVSAAFTASNFLEELGVKHYY
VVGNVATYRVIMDRIGIPEETQDLVLHLIDKDRVGEALQVLRDKTSREDIVELFGRLLNSGIDEALSVIMDFKGLGEGYS
IIESELAKTREFIELLNQVGFNAAYEPKLVRGLAYYTGLIFEYKTSGGLSVSIGGGGRYDGLTRVYGGEYEYSTGLALGL
DRIMLLLDPGKILPSKTTVLISILEGVPLKAGYELVRRIRVKGVEVIPYRARSLSKALSLANKINAGLVVIIGRREYESG
SITVKNMITGEQKYIGLNDINAFLDYIKGAGAQQPQDDAG

Sequences:

>Translated_440_residues
MSEEDVLNPPRGMRDLTGVDAELHEYMVELFKETARRNGFKPIITPTVEYFRLFEKKSGEEIKRSMYVFQDKAGRTLALR
PEVTASVVRAYLKEMRSQPKPIRLYYVAQCFRYEEPQKARYREFWQGGLEIIGDPDINADVSAAFTASNFLEELGVKHYY
VVGNVATYRVIMDRIGIPEETQDLVLHLIDKDRVGEALQVLRDKTSREDIVELFGRLLNSGIDEALSVIMDFKGLGEGYS
IIESELAKTREFIELLNQVGFNAAYEPKLVRGLAYYTGLIFEYKTSGGLSVSIGGGGRYDGLTRVYGGEYEYSTGLALGL
DRIMLLLDPGKILPSKTTVLISILEGVPLKAGYELVRRIRVKGVEVIPYRARSLSKALSLANKINAGLVVIIGRREYESG
SITVKNMITGEQKYIGLNDINAFLDYIKGAGAQQPQDDAG
>Mature_439_residues
SEEDVLNPPRGMRDLTGVDAELHEYMVELFKETARRNGFKPIITPTVEYFRLFEKKSGEEIKRSMYVFQDKAGRTLALRP
EVTASVVRAYLKEMRSQPKPIRLYYVAQCFRYEEPQKARYREFWQGGLEIIGDPDINADVSAAFTASNFLEELGVKHYYV
VGNVATYRVIMDRIGIPEETQDLVLHLIDKDRVGEALQVLRDKTSREDIVELFGRLLNSGIDEALSVIMDFKGLGEGYSI
IESELAKTREFIELLNQVGFNAAYEPKLVRGLAYYTGLIFEYKTSGGLSVSIGGGGRYDGLTRVYGGEYEYSTGLALGLD
RIMLLLDPGKILPSKTTVLISILEGVPLKAGYELVRRIRVKGVEVIPYRARSLSKALSLANKINAGLVVIIGRREYESGS
ITVKNMITGEQKYIGLNDINAFLDYIKGAGAQQPQDDAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=452, Percent_Identity=26.1061946902655, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI6996014, Length=453, Percent_Identity=26.0485651214128, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788861, Length=436, Percent_Identity=28.2110091743119, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71993686, Length=470, Percent_Identity=25.7446808510638, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=25.7446808510638, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=24.72647702407, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24643061, Length=477, Percent_Identity=23.2704402515723, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49294; Mature: 49163

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEDVLNPPRGMRDLTGVDAELHEYMVELFKETARRNGFKPIITPTVEYFRLFEKKSGE
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHH
EIKRSMYVFQDKAGRTLALRPEVTASVVRAYLKEMRSQPKPIRLYYVAQCFRYEEPQKAR
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCHHHH
YREFWQGGLEIIGDPDINADVSAAFTASNFLEELGVKHYYVVGNVATYRVIMDRIGIPEE
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCC
TQDLVLHLIDKDRVGEALQVLRDKTSREDIVELFGRLLNSGIDEALSVIMDFKGLGEGYS
HHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHH
IIESELAKTREFIELLNQVGFNAAYEPKLVRGLAYYTGLIFEYKTSGGLSVSIGGGGRYD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCC
GLTRVYGGEYEYSTGLALGLDRIMLLLDPGKILPSKTTVLISILEGVPLKAGYELVRRIR
CCHHHCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VKGVEVIPYRARSLSKALSLANKINAGLVVIIGRREYESGSITVKNMITGEQKYIGLNDI
HCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCEECHHHH
NAFLDYIKGAGAQQPQDDAG
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SEEDVLNPPRGMRDLTGVDAELHEYMVELFKETARRNGFKPIITPTVEYFRLFEKKSGE
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHH
EIKRSMYVFQDKAGRTLALRPEVTASVVRAYLKEMRSQPKPIRLYYVAQCFRYEEPQKAR
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCHHHH
YREFWQGGLEIIGDPDINADVSAAFTASNFLEELGVKHYYVVGNVATYRVIMDRIGIPEE
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCC
TQDLVLHLIDKDRVGEALQVLRDKTSREDIVELFGRLLNSGIDEALSVIMDFKGLGEGYS
HHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHH
IIESELAKTREFIELLNQVGFNAAYEPKLVRGLAYYTGLIFEYKTSGGLSVSIGGGGRYD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCCCCC
GLTRVYGGEYEYSTGLALGLDRIMLLLDPGKILPSKTTVLISILEGVPLKAGYELVRRIR
CCHHHCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VKGVEVIPYRARSLSKALSLANKINAGLVVIIGRREYESGSITVKNMITGEQKYIGLNDI
HCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCEECHHHH
NAFLDYIKGAGAQQPQDDAG
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA