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The map label for this gene is rffG [C]

Identifier: 218884406

GI number: 218884406

Start: 1034781

End: 1035704

Strand: Reverse

Name: rffG [C]

Synonym: DKAM_1095

Alternate gene names: 218884406

Gene position: 1035704-1034781 (Counterclockwise)

Preceding gene: 218884408

Following gene: 218884405

Centisome position: 75.86

GC content: 42.86

Gene sequence:

>924_bases
ATGAGAATTCTTGTCACTGGTGGAGGAGGCTTCATCGGTAGATTCCTAGTTAGCGACCTAGTGAAAAAAGGATATGATGC
CATCGTTGTAGACCGTGGACCCTCCCCCTTCGTAGAGCATCAGAGGATAAAATACTATGTTGGGGATGTGACAAATGTTA
TTCAAATTAATAATATCATGGCTAAGCATAAACCCGATGTAGTAATACACTTAGCCGCGTTGCTCGCCGATACATGTGAG
ATAGAACCTCTTCAAGCCACGAAAGTAAACATAGAGGCCACCCAAAACCTGATCGAACTAAGCATAACTCATGGCATCAA
GAGATTTGTATTCATGAGCTCAGCTTCAGTCTACCACCCTGATACACCTGAACCAGTAAGAGAAGAGGACGCAGGCAAAC
CAGTCTCATATTACGGAGTCACTAAGTATGCCGGGGAATTAATTGGATCGTGGTACTATAGAAAAGGACTGATAGACTTT
AGAGCTCTTAGACCCACTGTGGTGTTTGGGCCTGGAAGGTTCAGGGGCCCTTCTGCAGAGTACTCGAGCATGATTATTGA
AAGAGCCCTCAATAATGAAAAAGTAATCGTGAAGAATCCCAACGATAAGGTGAACTATATATATGTGAGAGACGTTGTGA
GTGTATTAATATTATTGGCCGAGGCGGAGAAAGTCAAGTATAGAGCCTATAATGCGGCTGGCTTTGTTAGCCGGGTAATA
GAGTTTGTTGAGATGGTTAAGAAGTATATTCCAACACTCCAATACGAGGTTCAACCACATGAAACAGTCAGATATGCAGC
GGTAATCGATGACTCTAGGATCAGGGAGGAGTTGGGGTGGAGGCCCCAGTACACATATGAAAAAGCTATTGAAGACTATA
TTGAAACAGTTAGGAAAGGTGAGGAATTATTTAGAGTGTATTAA

Upstream 100 bases:

>100_bases
CATATTGCTAGAGCTAATAAAACAGGTTTTATTGTTTACTTAAACATGGCTATTGTAATATCCTGTAGTTGAATACATAT
ATCATGGAGTGTGTCTAGCT

Downstream 100 bases:

>100_bases
AAGGGGCGGGATAATGTTAAGTCTCAATGGTAAAGTAGCGTTAGTAACCGGTGGGGGAAAAGGTATTGGCCGTGAGATAT
CCTTGGAGCTAGCCAATCAT

Product: UDP-glucose 4-epimerase (galE-2)

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRILVTGGGGFIGRFLVSDLVKKGYDAIVVDRGPSPFVEHQRIKYYVGDVTNVIQINNIMAKHKPDVVIHLAALLADTCE
IEPLQATKVNIEATQNLIELSITHGIKRFVFMSSASVYHPDTPEPVREEDAGKPVSYYGVTKYAGELIGSWYYRKGLIDF
RALRPTVVFGPGRFRGPSAEYSSMIIERALNNEKVIVKNPNDKVNYIYVRDVVSVLILLAEAEKVKYRAYNAAGFVSRVI
EFVEMVKKYIPTLQYEVQPHETVRYAAVIDDSRIREELGWRPQYTYEKAIEDYIETVRKGEELFRVY

Sequences:

>Translated_307_residues
MRILVTGGGGFIGRFLVSDLVKKGYDAIVVDRGPSPFVEHQRIKYYVGDVTNVIQINNIMAKHKPDVVIHLAALLADTCE
IEPLQATKVNIEATQNLIELSITHGIKRFVFMSSASVYHPDTPEPVREEDAGKPVSYYGVTKYAGELIGSWYYRKGLIDF
RALRPTVVFGPGRFRGPSAEYSSMIIERALNNEKVIVKNPNDKVNYIYVRDVVSVLILLAEAEKVKYRAYNAAGFVSRVI
EFVEMVKKYIPTLQYEVQPHETVRYAAVIDDSRIREELGWRPQYTYEKAIEDYIETVRKGEELFRVY
>Mature_307_residues
MRILVTGGGGFIGRFLVSDLVKKGYDAIVVDRGPSPFVEHQRIKYYVGDVTNVIQINNIMAKHKPDVVIHLAALLADTCE
IEPLQATKVNIEATQNLIELSITHGIKRFVFMSSASVYHPDTPEPVREEDAGKPVSYYGVTKYAGELIGSWYYRKGLIDF
RALRPTVVFGPGRFRGPSAEYSSMIIERALNNEKVIVKNPNDKVNYIYVRDVVSVLILLAEAEKVKYRAYNAAGFVSRVI
EFVEMVKKYIPTLQYEVQPHETVRYAAVIDDSRIREELGWRPQYTYEKAIEDYIETVRKGEELFRVY

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=22.5705329153605, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI193211614, Length=226, Percent_Identity=29.2035398230088, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI8393516, Length=226, Percent_Identity=29.2035398230088, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI56237023, Length=333, Percent_Identity=26.4264264264264, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI56118217, Length=333, Percent_Identity=26.4264264264264, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI189083684, Length=333, Percent_Identity=26.4264264264264, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=24.6056782334385, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=26.647564469914, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786974, Length=333, Percent_Identity=27.6276276276276, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566934, Length=316, Percent_Identity=25.6329113924051, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=355, Percent_Identity=27.887323943662, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=27.7310924369748, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=161, Percent_Identity=35.4037267080745, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667531, Length=322, Percent_Identity=28.2608695652174, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19923002, Length=334, Percent_Identity=26.3473053892216, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 35010; Mature: 35010

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGGGFIGRFLVSDLVKKGYDAIVVDRGPSPFVEHQRIKYYVGDVTNVIQINNIM
CEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHH
AKHKPDVVIHLAALLADTCEIEPLQATKVNIEATQNLIELSITHGIKRFVFMSSASVYHP
HCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
DTPEPVREEDAGKPVSYYGVTKYAGELIGSWYYRKGLIDFRALRPTVVFGPGRFRGPSAE
CCCCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCCCCCCHH
YSSMIIERALNNEKVIVKNPNDKVNYIYVRDVVSVLILLAEAEKVKYRAYNAAGFVSRVI
HHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
EFVEMVKKYIPTLQYEVQPHETVRYAAVIDDSRIREELGWRPQYTYEKAIEDYIETVRKG
HHHHHHHHHCCCEEEEECCCHHEEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
EELFRVY
HHHHHCC
>Mature Secondary Structure
MRILVTGGGGFIGRFLVSDLVKKGYDAIVVDRGPSPFVEHQRIKYYVGDVTNVIQINNIM
CEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHH
AKHKPDVVIHLAALLADTCEIEPLQATKVNIEATQNLIELSITHGIKRFVFMSSASVYHP
HCCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
DTPEPVREEDAGKPVSYYGVTKYAGELIGSWYYRKGLIDFRALRPTVVFGPGRFRGPSAE
CCCCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCCCCCCHH
YSSMIIERALNNEKVIVKNPNDKVNYIYVRDVVSVLILLAEAEKVKYRAYNAAGFVSRVI
HHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
EFVEMVKKYIPTLQYEVQPHETVRYAAVIDDSRIREELGWRPQYTYEKAIEDYIETVRKG
HHHHHHHHHCCCEEEEECCCHHEEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
EELFRVY
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA