Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 218884312

GI number: 218884312

Start: 946943

End: 948382

Strand: Reverse

Name: proS [H]

Synonym: DKAM_1001

Alternate gene names: 218884312

Gene position: 948382-946943 (Counterclockwise)

Preceding gene: 218884313

Following gene: 218884311

Centisome position: 69.47

GC content: 44.38

Gene sequence:

>1440_bases
ATGAGCATTAGGAAAAGCATTGGGATAGATAAATATGAAAACTTCCCCGAGTGGTATCAGGCAGTATTAAAGGAAGCCGG
CGTCATCGATATAAGATACCCTGTTAAAGGCATGTATGTATGGATGCCATACGGATTGAAAGCATTAAGGCTCACCCAGA
GGATAATAGTGGATCTCCTTGAGAAAACAGGACACCAAGAGGCATACTTCCCTACAATGGTGCCAGAAAGCGTGTTTTCA
CGTGAAAAAGATTTTCTACAGGGTTTCGGAGGAGAAACATTCATTGTTGAGGGAACAATGACGAGGAGGTTTAATGAGAG
ATTATTCGTTAGGCCAACCAGTGAAACAGTAATGTATTACATGTGGAACATGTGGATAAAGGGGAGGAAGGATCTTCCAT
TGAAAATGTATCAAGTAGTAAACGTGTTCAGGTATGAGACAAAGATGACTCATCCAATACTAAGGGTAAGAGAGATAATG
GGCTTCATAGAGGCTCATACGGCACACCACTCGAGAGAAGAGGCCGAGAAACAGATAATCGAGGCCATAGGGATATATAA
GGAGTTCTTCAATAGGCTTCAGCTACCCTACTTCATAGTCAAGACACCTCCATGGGACACGTTTGCTGGTGCAGAGTATA
ATTACGACTTCATAACGGTTATGCCTGATGGAAAAGGCCTCGAGCTAGGTAGCGTTATAAATCTAGGGCAGAAATTCGCT
GGGGCATTCGAGATAAAATTCATGGATACTGATGGAGAAGAGAAATACGTCTACCAGACATGTTATGGTGTGAGCGAGAG
AGTTCTAGGCGCGGTTATAGGAATACATGGAGACGAGGTAGGATTATTCTTCCCGCCAGAAATCGCACCAATACAGGTCG
TCATAGTGCCAATAATGAAGCCTGGTGAAAAAGAGATTCTTGAATACGCTTTAAAGATAGGAAGCATGCTGGAGTCACAT
GGCTTAAGAGTCTTCATAGACAGCGACCTAGAGCATACGCCTGGATGGAAATACTATTACTGGGAAATGAAGGGAGTGCC
AACCAGGATAGAGGTAGGGGCTAGGGAGGTTGAGACATCAACAGTAACCATTGCTAGGCGGGATAAAAGAGAGAAGCAGG
TAGTTAGGCTAGAGGAGGTCCTTGAAACCCTCCGCAAGATATGGTCCGAGATAAACGAGTACCTGAGGATTAAGGGGTTA
GAGCACCTTAAGAAGAAAACAATATTCTTACCGGATTATCTTGAGAATAAGGGGTTCAATGGATTAATCATCGCTCCCTG
GGATGGAAGCAAGGGATGTGCTGAGGAACTCGAGAAAGAGACAGGCAAGAACGTCCTCGGAGAGATAGTCGAGTCCCCGA
TACCTCTCCCTTCACCTACCCAGTACATGGCGTGTAGTAATAAAGCTGATAGATATGCCCTGCTAGGTGTAACATATTAG

Upstream 100 bases:

>100_bases
CTTAAAAGAATAGAGTCCTTCATGCTTATTAAGATTGATGAATTGTTAAATAACATACTAGAACTACAGGTATGTAGGAG
AAGTATACAGGGTGCAAGCG

Downstream 100 bases:

>100_bases
GCCCAATGGGTTTCCAACTAGCTGACCATATTCTTGATTAGAAAGGATTAGAAAAACGGAACCATTAACTTTTAAAGTCC
ATAATTAAATCCTTTAATTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSIRKSIGIDKYENFPEWYQAVLKEAGVIDIRYPVKGMYVWMPYGLKALRLTQRIIVDLLEKTGHQEAYFPTMVPESVFS
REKDFLQGFGGETFIVEGTMTRRFNERLFVRPTSETVMYYMWNMWIKGRKDLPLKMYQVVNVFRYETKMTHPILRVREIM
GFIEAHTAHHSREEAEKQIIEAIGIYKEFFNRLQLPYFIVKTPPWDTFAGAEYNYDFITVMPDGKGLELGSVINLGQKFA
GAFEIKFMDTDGEEKYVYQTCYGVSERVLGAVIGIHGDEVGLFFPPEIAPIQVVIVPIMKPGEKEILEYALKIGSMLESH
GLRVFIDSDLEHTPGWKYYYWEMKGVPTRIEVGAREVETSTVTIARRDKREKQVVRLEEVLETLRKIWSEINEYLRIKGL
EHLKKKTIFLPDYLENKGFNGLIIAPWDGSKGCAEELEKETGKNVLGEIVESPIPLPSPTQYMACSNKADRYALLGVTY

Sequences:

>Translated_479_residues
MSIRKSIGIDKYENFPEWYQAVLKEAGVIDIRYPVKGMYVWMPYGLKALRLTQRIIVDLLEKTGHQEAYFPTMVPESVFS
REKDFLQGFGGETFIVEGTMTRRFNERLFVRPTSETVMYYMWNMWIKGRKDLPLKMYQVVNVFRYETKMTHPILRVREIM
GFIEAHTAHHSREEAEKQIIEAIGIYKEFFNRLQLPYFIVKTPPWDTFAGAEYNYDFITVMPDGKGLELGSVINLGQKFA
GAFEIKFMDTDGEEKYVYQTCYGVSERVLGAVIGIHGDEVGLFFPPEIAPIQVVIVPIMKPGEKEILEYALKIGSMLESH
GLRVFIDSDLEHTPGWKYYYWEMKGVPTRIEVGAREVETSTVTIARRDKREKQVVRLEEVLETLRKIWSEINEYLRIKGL
EHLKKKTIFLPDYLENKGFNGLIIAPWDGSKGCAEELEKETGKNVLGEIVESPIPLPSPTQYMACSNKADRYALLGVTY
>Mature_478_residues
SIRKSIGIDKYENFPEWYQAVLKEAGVIDIRYPVKGMYVWMPYGLKALRLTQRIIVDLLEKTGHQEAYFPTMVPESVFSR
EKDFLQGFGGETFIVEGTMTRRFNERLFVRPTSETVMYYMWNMWIKGRKDLPLKMYQVVNVFRYETKMTHPILRVREIMG
FIEAHTAHHSREEAEKQIIEAIGIYKEFFNRLQLPYFIVKTPPWDTFAGAEYNYDFITVMPDGKGLELGSVINLGQKFAG
AFEIKFMDTDGEEKYVYQTCYGVSERVLGAVIGIHGDEVGLFFPPEIAPIQVVIVPIMKPGEKEILEYALKIGSMLESHG
LRVFIDSDLEHTPGWKYYYWEMKGVPTRIEVGAREVETSTVTIARRDKREKQVVRLEEVLETLRKIWSEINEYLRIKGLE
HLKKKTIFLPDYLENKGFNGLIIAPWDGSKGCAEELEKETGKNVLGEIVESPIPLPSPTQYMACSNKADRYALLGVTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=508, Percent_Identity=29.3307086614173, Blast_Score=225, Evalue=9e-59,
Organism=Escherichia coli, GI1786392, Length=173, Percent_Identity=31.2138728323699, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71991342, Length=500, Percent_Identity=31.8, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI71991346, Length=367, Percent_Identity=32.425068119891, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321808, Length=457, Percent_Identity=32.1663019693654, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24649466, Length=498, Percent_Identity=29.5180722891566, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24649468, Length=498, Percent_Identity=29.5180722891566, Blast_Score=235, Evalue=6e-62,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 55400; Mature: 55269

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRKSIGIDKYENFPEWYQAVLKEAGVIDIRYPVKGMYVWMPYGLKALRLTQRIIVDLL
CCCCHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
EKTGHQEAYFPTMVPESVFSREKDFLQGFGGETFIVEGTMTRRFNERLFVRPTSETVMYY
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCEEEEEECHHHHHHE
MWNMWIKGRKDLPLKMYQVVNVFRYETKMTHPILRVREIMGFIEAHTAHHSREEAEKQII
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EAIGIYKEFFNRLQLPYFIVKTPPWDTFAGAEYNYDFITVMPDGKGLELGSVINLGQKFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHCC
GAFEIKFMDTDGEEKYVYQTCYGVSERVLGAVIGIHGDEVGLFFPPEIAPIQVVIVPIMK
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC
PGEKEILEYALKIGSMLESHGLRVFIDSDLEHTPGWKYYYWEMKGVPTRIEVGAREVETS
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCCEEEECCCEEEECE
TVTIARRDKREKQVVRLEEVLETLRKIWSEINEYLRIKGLEHLKKKTIFLPDYLENKGFN
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCEEECCHHHHCCCCC
GLIIAPWDGSKGCAEELEKETGKNVLGEIVESPIPLPSPTQYMACSNKADRYALLGVTY
EEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECC
>Mature Secondary Structure 
SIRKSIGIDKYENFPEWYQAVLKEAGVIDIRYPVKGMYVWMPYGLKALRLTQRIIVDLL
CCCHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
EKTGHQEAYFPTMVPESVFSREKDFLQGFGGETFIVEGTMTRRFNERLFVRPTSETVMYY
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCEEEEEECHHHHHHE
MWNMWIKGRKDLPLKMYQVVNVFRYETKMTHPILRVREIMGFIEAHTAHHSREEAEKQII
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EAIGIYKEFFNRLQLPYFIVKTPPWDTFAGAEYNYDFITVMPDGKGLELGSVINLGQKFA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHCC
GAFEIKFMDTDGEEKYVYQTCYGVSERVLGAVIGIHGDEVGLFFPPEIAPIQVVIVPIMK
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC
PGEKEILEYALKIGSMLESHGLRVFIDSDLEHTPGWKYYYWEMKGVPTRIEVGAREVETS
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCCEEEECCCEEEECE
TVTIARRDKREKQVVRLEEVLETLRKIWSEINEYLRIKGLEHLKKKTIFLPDYLENKGFN
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCEEECCHHHHCCCCC
GLIIAPWDGSKGCAEELEKETGKNVLGEIVESPIPLPSPTQYMACSNKADRYALLGVTY
EEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]