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The map label for this gene is alaXM [H]

Identifier: 218883373

GI number: 218883373

Start: 55133

End: 55840

Strand: Reverse

Name: alaXM [H]

Synonym: DKAM_0059

Alternate gene names: 218883373

Gene position: 55840-55133 (Counterclockwise)

Preceding gene: 218883374

Following gene: 218883371

Centisome position: 4.09

GC content: 43.36

Gene sequence:

>708_bases
GTGTATCAGGGCGACCCGTATTTGAAAGAATATATGGCCACTATTAAAGCCATTAAGGGAAGCAGGGTATTCCTTGATAA
GACAATATTCCATCCACGCAGTGGAGGCGTTGAAAACGATACTGGTTACATGGTTTTCAATGAGGGGATGGTGAGGGTTG
TAAATGTATATTATGATAAGGAGTCCGGGGATGTAGCCCACGAGGTTGACAATGCATCGCTACTTCCTCAACCTGGATCA
AGCGTTAAACTAGTTTTAGATTGGGAAAGGAGGTATAGGCTTATGAGGCTTCATACGGCTGCCCATATATTATCCGCTAT
AATGTATACTAATTACAATGCATTAATCACAGGCGGGAACATCACGCCTGAATACGGGTACGATGATTACAGTTTAACAG
AGTTCAGTCGTGAAATATTCGAGGAAGCCGTGAAGAAGGCGAACGAAGTGGTAGCAAGAAATATCGAGGTCAAAATATAT
TGGTTACCCAGAGAGGAGGCCCTGAGAATACCGGGAGTTGTTAAGCTAGCTAGTAAAATGCCCCCTGAAGTAGAGAAGCT
AAGAATCGTTGAAATACCGGGAGTAGATATACAAGCTGATGGAGGACCACATGTCAAGTCGACTGGTGAAATCGGCGTGA
TCAAATTACTTAGAGTCGAGAACAAGGGTAAGAATAAGAAGAGGCTATACTTCAGTGTTGAACCGTGA

Upstream 100 bases:

>100_bases
GAGGAAACCCCGTGACCGGGGTTCGAATCCCCGCCCGGCTACCTTTTATTACTCATCATTTAGTAATAATGGTTAGGGTT
GATACGTTTTGACTGAGCTT

Downstream 100 bases:

>100_bases
TCACGATTCTGGTTTCCAAGCCGACTCTAACTCAAAGACCCTGGGAACCGTGCTTCCTCTTACAGTAGTCAAGTATTCCC
CCCACATCCACTCCTTTTAA

Product: Threonyl/alanyl tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MYQGDPYLKEYMATIKAIKGSRVFLDKTIFHPRSGGVENDTGYMVFNEGMVRVVNVYYDKESGDVAHEVDNASLLPQPGS
SVKLVLDWERRYRLMRLHTAAHILSAIMYTNYNALITGGNITPEYGYDDYSLTEFSREIFEEAVKKANEVVARNIEVKIY
WLPREEALRIPGVVKLASKMPPEVEKLRIVEIPGVDIQADGGPHVKSTGEIGVIKLLRVENKGKNKKRLYFSVEP

Sequences:

>Translated_235_residues
MYQGDPYLKEYMATIKAIKGSRVFLDKTIFHPRSGGVENDTGYMVFNEGMVRVVNVYYDKESGDVAHEVDNASLLPQPGS
SVKLVLDWERRYRLMRLHTAAHILSAIMYTNYNALITGGNITPEYGYDDYSLTEFSREIFEEAVKKANEVVARNIEVKIY
WLPREEALRIPGVVKLASKMPPEVEKLRIVEIPGVDIQADGGPHVKSTGEIGVIKLLRVENKGKNKKRLYFSVEP
>Mature_235_residues
MYQGDPYLKEYMATIKAIKGSRVFLDKTIFHPRSGGVENDTGYMVFNEGMVRVVNVYYDKESGDVAHEVDNASLLPQPGS
SVKLVLDWERRYRLMRLHTAAHILSAIMYTNYNALITGGNITPEYGYDDYSLTEFSREIFEEAVKKANEVVARNIEVKIY
WLPREEALRIPGVVKLASKMPPEVEKLRIVEIPGVDIQADGGPHVKSTGEIGVIKLLRVENKGKNKKRLYFSVEP

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI13376886, Length=219, Percent_Identity=27.3972602739726, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324288, Length=239, Percent_Identity=27.6150627615063, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 26642; Mature: 26642

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQGDPYLKEYMATIKAIKGSRVFLDKTIFHPRSGGVENDTGYMVFNEGMVRVVNVYYDK
CCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEECCCEEEEEEEEEEC
ESGDVAHEVDNASLLPQPGSSVKLVLDWERRYRLMRLHTAAHILSAIMYTNYNALITGGN
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ITPEYGYDDYSLTEFSREIFEEAVKKANEVVARNIEVKIYWLPREEALRIPGVVKLASKM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHCCCCCCHHHHHHHC
PPEVEKLRIVEIPGVDIQADGGPHVKSTGEIGVIKLLRVENKGKNKKRLYFSVEP
CCCHHHEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MYQGDPYLKEYMATIKAIKGSRVFLDKTIFHPRSGGVENDTGYMVFNEGMVRVVNVYYDK
CCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEECCCEEEEEEEEEEC
ESGDVAHEVDNASLLPQPGSSVKLVLDWERRYRLMRLHTAAHILSAIMYTNYNALITGGN
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ITPEYGYDDYSLTEFSREIFEEAVKKANEVVARNIEVKIYWLPREEALRIPGVVKLASKM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHHCCCCCCHHHHHHHC
PPEVEKLRIVEIPGVDIQADGGPHVKSTGEIGVIKLLRVENKGKNKKRLYFSVEP
CCCHHHEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]