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The map label for this gene is sufB [H]

Identifier: 218883320

GI number: 218883320

Start: 3470

End: 4876

Strand: Reverse

Name: sufB [H]

Synonym: DKAM_0006

Alternate gene names: 218883320

Gene position: 4876-3470 (Counterclockwise)

Preceding gene: 218883321

Following gene: 218883319

Centisome position: 0.36

GC content: 45.06

Gene sequence:

>1407_bases
ATGGCTATCGAGCTATCTAAGAGTCTTGAACACCATGAGATCGAGCCTGTTGAATACAGAAAGGAGATAGAGATCAAGGG
CAGACTATCTAGAAGTATTGTAGAGGAGATCTCAAGAGTGAAAAAAGAACCAGAATGGATGAGGCTTTATAGGCTTAAGG
CGCTGGAGGCATTTGAAAAATTCCCCTCGCCTAGATGGCTTCCTTGGATTGAGAGCATAGACCTCGATGAGATAGCTTCA
TACTATGTTAAACCAGCAGCTTCAACAGTAAGCAATTTCGATGAACTACCCAGCGAGATCCGTAGAATATATGAGAGGCT
TGGATTACCGGAGACATACGCCAAGTACCTGGCTGGGCTTTTAACCGCGCTCGATAGTGAAAACATATATACTGCAATGA
AGGACTTACTCAAGAAGCTCGGGGTAATAATGCTTCCTATGGAGGAGGCGATCCAGAAATACCCAGACATCGTTAAGAAA
TACTTCGGTAGGGTATTCCCGTACACAGACCACAAGTTTGCCGCGCTGCATCATGCCCTCTGGAGTGGCGGAGTATTCGT
CTATGTCCCGAGAGGCGTTAAAGTACCCTACCCGGTTGAAGCATTTTTCTTCATTGGAAGCGAGCTAGAGGGGCAGTTCG
AGCACACAATTGTAGTTGGCGATGAGGGGAGCGAGATAACGTTCATTGAGGGTTGTAGCGCGCCAAGGCTGAAGAGGCAT
AGCTTCCATGATGGTATGGTTGAGCTCTACGCTCATAAGGGATCCAGGATAAACTTTGTAACGGTTCAGAACTGGAGCAG
GAACATAGTGAACTTCAATAATAAGAGAGGTATTGCAGAGGATGGAGCCATTATAGAATGGGTTGAGGGAAGTATAGGCA
GTAAAATAACCGTGACATATCCGTCTACGATATTGAAGGGGAGGGGCTCGAAGACGACGAGCACCGTGATAGGTATAAGT
AATGGTAATGTGGTTAAGGACGCCGGGTCGAAGATTATTCATGCCGCACCCTATACTAGTAGCAGGATAATATCTAAGAG
TATTAGCAGTAACGGGGGAATAAATATATATAGAGGACTAGTACAGGTGAATAAGGGAGCCCTTAATGTAAAGAGCTATA
CGCAATGCGACAGCCTCATACTCGACGATAAGAGCTCTAGTAGTACATATCCGATAGAGCACATTATGGAGAAGGATGCT
GAAGTCGCTCATGAAGCAACTACGTTCAGGATAACTGAGGATCAGTTATTCTACATGACTTCTAGGGGTCTAAGCGAGAA
GGAAGCTGTCTCACTCATAGTACTCGGATTTGTTAAGGATATTTTTCCAAAGCTACCATTCGAGTATGCATCGATGTTGA
CGAAGGTTATACAGTTAGAATTCAAGGAGATCGGGGGGGTTGGATAA

Upstream 100 bases:

>100_bases
CATCTATGTCATGCTGAATGGAAGGATAGTTGCGGAGGGCGGGTTAGAAATAGCTGATAAAATAGCTAGGGAGGGATACA
ACTGGTTGAGGTAAAGTAGT

Downstream 100 bases:

>100_bases
TGGCTAAGACGGTTGTAAGAGGGGCGGGGGACTCACCAACCATTAAAAAATATATTGATAAATCCCCGCTTGAAGAACTT
GTTCGGAGAGCATTAAACAG

Product: FeS assembly protein SufB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAIELSKSLEHHEIEPVEYRKEIEIKGRLSRSIVEEISRVKKEPEWMRLYRLKALEAFEKFPSPRWLPWIESIDLDEIAS
YYVKPAASTVSNFDELPSEIRRIYERLGLPETYAKYLAGLLTALDSENIYTAMKDLLKKLGVIMLPMEEAIQKYPDIVKK
YFGRVFPYTDHKFAALHHALWSGGVFVYVPRGVKVPYPVEAFFFIGSELEGQFEHTIVVGDEGSEITFIEGCSAPRLKRH
SFHDGMVELYAHKGSRINFVTVQNWSRNIVNFNNKRGIAEDGAIIEWVEGSIGSKITVTYPSTILKGRGSKTTSTVIGIS
NGNVVKDAGSKIIHAAPYTSSRIISKSISSNGGINIYRGLVQVNKGALNVKSYTQCDSLILDDKSSSSTYPIEHIMEKDA
EVAHEATTFRITEDQLFYMTSRGLSEKEAVSLIVLGFVKDIFPKLPFEYASMLTKVIQLEFKEIGGVG

Sequences:

>Translated_468_residues
MAIELSKSLEHHEIEPVEYRKEIEIKGRLSRSIVEEISRVKKEPEWMRLYRLKALEAFEKFPSPRWLPWIESIDLDEIAS
YYVKPAASTVSNFDELPSEIRRIYERLGLPETYAKYLAGLLTALDSENIYTAMKDLLKKLGVIMLPMEEAIQKYPDIVKK
YFGRVFPYTDHKFAALHHALWSGGVFVYVPRGVKVPYPVEAFFFIGSELEGQFEHTIVVGDEGSEITFIEGCSAPRLKRH
SFHDGMVELYAHKGSRINFVTVQNWSRNIVNFNNKRGIAEDGAIIEWVEGSIGSKITVTYPSTILKGRGSKTTSTVIGIS
NGNVVKDAGSKIIHAAPYTSSRIISKSISSNGGINIYRGLVQVNKGALNVKSYTQCDSLILDDKSSSSTYPIEHIMEKDA
EVAHEATTFRITEDQLFYMTSRGLSEKEAVSLIVLGFVKDIFPKLPFEYASMLTKVIQLEFKEIGGVG
>Mature_467_residues
AIELSKSLEHHEIEPVEYRKEIEIKGRLSRSIVEEISRVKKEPEWMRLYRLKALEAFEKFPSPRWLPWIESIDLDEIASY
YVKPAASTVSNFDELPSEIRRIYERLGLPETYAKYLAGLLTALDSENIYTAMKDLLKKLGVIMLPMEEAIQKYPDIVKKY
FGRVFPYTDHKFAALHHALWSGGVFVYVPRGVKVPYPVEAFFFIGSELEGQFEHTIVVGDEGSEITFIEGCSAPRLKRHS
FHDGMVELYAHKGSRINFVTVQNWSRNIVNFNNKRGIAEDGAIIEWVEGSIGSKITVTYPSTILKGRGSKTTSTVIGISN
GNVVKDAGSKIIHAAPYTSSRIISKSISSNGGINIYRGLVQVNKGALNVKSYTQCDSLILDDKSSSSTYPIEHIMEKDAE
VAHEATTFRITEDQLFYMTSRGLSEKEAVSLIVLGFVKDIFPKLPFEYASMLTKVIQLEFKEIGGVG

Specific function: The sufBCD complex acts synergistically with sufE to stimulate the cysteine desulfurase activity of sufS. The sufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake fro

COG id: COG0719

COG function: function code O; ABC-type transport system involved in Fe-S cluster assembly, permease component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0051 (ycf24) family [H]

Homologues:

Organism=Escherichia coli, GI87081955, Length=464, Percent_Identity=36.6379310344828, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787971, Length=157, Percent_Identity=26.1146496815287, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010231
- InterPro:   IPR000825 [H]

Pfam domain/function: PF01458 UPF0051 [H]

EC number: NA

Molecular weight: Translated: 52731; Mature: 52600

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIELSKSLEHHEIEPVEYRKEIEIKGRLSRSIVEEISRVKKEPEWMRLYRLKALEAFEK
CEEECCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FPSPRWLPWIESIDLDEIASYYVKPAASTVSNFDELPSEIRRIYERLGLPETYAKYLAGL
CCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTALDSENIYTAMKDLLKKLGVIMLPMEEAIQKYPDIVKKYFGRVFPYTDHKFAALHHAL
HHHHCCCHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
WSGGVFVYVPRGVKVPYPVEAFFFIGSELEGQFEHTIVVGDEGSEITFIEGCSAPRLKRH
HCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHC
SFHDGMVELYAHKGSRINFVTVQNWSRNIVNFNNKRGIAEDGAIIEWVEGSIGSKITVTY
CCCCCHHHHEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEC
PSTILKGRGSKTTSTVIGISNGNVVKDAGSKIIHAAPYTSSRIISKSISSNGGINIYRGL
CHHHHCCCCCCCCEEEEECCCCCEEECCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHH
VQVNKGALNVKSYTQCDSLILDDKSSSSTYPIEHIMEKDAEVAHEATTFRITEDQLFYMT
HHHCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCEEEEEE
SRGLSEKEAVSLIVLGFVKDIFPKLPFEYASMLTKVIQLEFKEIGGVG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIELSKSLEHHEIEPVEYRKEIEIKGRLSRSIVEEISRVKKEPEWMRLYRLKALEAFEK
EEECCCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FPSPRWLPWIESIDLDEIASYYVKPAASTVSNFDELPSEIRRIYERLGLPETYAKYLAGL
CCCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTALDSENIYTAMKDLLKKLGVIMLPMEEAIQKYPDIVKKYFGRVFPYTDHKFAALHHAL
HHHHCCCHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
WSGGVFVYVPRGVKVPYPVEAFFFIGSELEGQFEHTIVVGDEGSEITFIEGCSAPRLKRH
HCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHHC
SFHDGMVELYAHKGSRINFVTVQNWSRNIVNFNNKRGIAEDGAIIEWVEGSIGSKITVTY
CCCCCHHHHEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEC
PSTILKGRGSKTTSTVIGISNGNVVKDAGSKIIHAAPYTSSRIISKSISSNGGINIYRGL
CHHHHCCCCCCCCEEEEECCCCCEEECCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHH
VQVNKGALNVKSYTQCDSLILDDKSSSSTYPIEHIMEKDAEVAHEATTFRITEDQLFYMT
HHHCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCEEEEEE
SRGLSEKEAVSLIVLGFVKDIFPKLPFEYASMLTKVIQLEFKEIGGVG
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]