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The map label for this gene is ilvA [H]

Identifier: 218711018

GI number: 218711018

Start: 3282244

End: 3283788

Strand: Direct

Name: ilvA [H]

Synonym: VS_3147

Alternate gene names: 218711018

Gene position: 3282244-3283788 (Clockwise)

Preceding gene: 218711017

Following gene: 218711020

Centisome position: 99.48

GC content: 48.54

Gene sequence:

>1545_bases
ATGAGTGATGACACCTCCAGCCCCCAAAAACAAACTGGCGCAGATTATCTGCGTCAGATCCTGAGAGCGCCGGTTTACGA
AGCGGCAATCGTGACACCTCTGCAGGATATGCCACGTTTAAGCGCGCGCATTGGTAATCAGGTTCAGCTTAAACGAGAAG
ACCGTCAGCCGGTGCACTCGTTCAAGCTACGTGGTGCCTACAACATGGTATCAAGCCTTTCTGAACAACAGAAAGCCGCA
GGTGTGATTGCTGCATCGGCAGGTAACCATGCTCAAGGTATGGCGCTGTCCGGTTCTAAGCTTGGTATTCAGACCACTAT
TGTGATGCCAAAAACGACGCCGGATATCAAGGTTGATGCGGTACGCGGATTTGGTGGTAACGTGGTTCTGCACGGCAGTA
ATTTCGATGAAGCAAAAGCGGAAGCGGAACGCCTATCGGCTGAACATGGCTTCACCTTTGTACCTCCTTTCGATCACCCA
CTGGTGATTGCAGGGCAAGGTACTATGGGCATGGAGATGCTGCAGCAAAACGGTCACATGGATTATATCTTTGTGCCGGT
TGGTGGTGGTGGCTTAGCCGCTGGTGTTGCAGTGCTTATCAAACAACTGATGCCAGAGATTAAAGTGATTGCGGTAGAGC
CTGAAGACTCAGCTTGCTTGAAAGCAGCACTGGATGCGGGTGAACCAGTGGTACTGGATCAGGTCAGCATGTTTGCTGAT
GGTGTTGCGGTTAAACGCATTGGTGAAGAAACCTTCCGTCTTTGCCAACAGTACATTGATGGTCATATTACAGTCTCTAG
CGATGAAATCTGCTCTGCGGTAAAGGACATATTTGAAGACACCCGTGCAATTGCTGAGCCTTCAGGAGCGCTTGCTCTGG
CAGGCCTTAAGAAGTTTGCAGAACAAAACCAACTGCAAAATAAGCAACTAGCAACGGTACTTTCTGGTGCGAACACCAAC
TTCCACGGTCTGCGTTATGTCTCTGAGCGTTGTGAATTGGGTGAGAAGCGAGAAGGTCTACTTGCGGTGACTATTCCAGA
ACGACAAGGTGCGTTCCTAGAGTTCTGTAACATTATTGGTGGTCGAGCGGTGACTGAGTTTAACTACCGCCACAACGACG
AAAGCCTCGCGAATATCTTCGTTGGTGTACGTCTACAAGGTGGACAAGAAGAACTCGAACACATCATCAATGACTTACGT
GAGGGTGACTACGCGGTTGTCGACTTGTCTGATGACGAGATGGCAAAACTTCATATTCGTTACATGATTGGCGGCAAACC
ATCGAAACCATTGAAAGAGCGTCTGTACAGCTTTGAGTTCCCTGAATACCCAGGAGCATTGATTAAATTCCTTGATACCT
TAGGGACTCACTGGAATATCAGCTTGTTCAACTATCGTAACCATGGTGCTGACTACGGTCGTGTGCTGTGTGGTTTTGAG
CTGGGTGATGACGATTTGGCTCAGTTCTCTACGCATTTAGAAGAACTCGGCTACCAATGCAAAGATGAAACCGATAACCC
TTCCTACAAGTTCTTCTTGTCTTAA

Upstream 100 bases:

>100_bases
GTCAGCGTGAAGTGTCTTTCGCACTGAAAGCTTATGCAAGCATGGCGACCAGTGCCGACAAAGGCGCTGTACGTGATAAG
TCTAAACTTGAGGGCTAACT

Downstream 100 bases:

>100_bases
GAGTTAACGACCGAATCAAAAAAGAGCGAGTATTTACTCGCTCTTTTTATTGGTGCTGATTCAGTTCAAGCTTGAAAAGG
ATCTTCTTGTAAATAGATCA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSDDTSSPQKQTGADYLRQILRAPVYEAAIVTPLQDMPRLSARIGNQVQLKREDRQPVHSFKLRGAYNMVSSLSEQQKAA
GVIAASAGNHAQGMALSGSKLGIQTTIVMPKTTPDIKVDAVRGFGGNVVLHGSNFDEAKAEAERLSAEHGFTFVPPFDHP
LVIAGQGTMGMEMLQQNGHMDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFAD
GVAVKRIGEETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFAEQNQLQNKQLATVLSGANTN
FHGLRYVSERCELGEKREGLLAVTIPERQGAFLEFCNIIGGRAVTEFNYRHNDESLANIFVGVRLQGGQEELEHIINDLR
EGDYAVVDLSDDEMAKLHIRYMIGGKPSKPLKERLYSFEFPEYPGALIKFLDTLGTHWNISLFNYRNHGADYGRVLCGFE
LGDDDLAQFSTHLEELGYQCKDETDNPSYKFFLS

Sequences:

>Translated_514_residues
MSDDTSSPQKQTGADYLRQILRAPVYEAAIVTPLQDMPRLSARIGNQVQLKREDRQPVHSFKLRGAYNMVSSLSEQQKAA
GVIAASAGNHAQGMALSGSKLGIQTTIVMPKTTPDIKVDAVRGFGGNVVLHGSNFDEAKAEAERLSAEHGFTFVPPFDHP
LVIAGQGTMGMEMLQQNGHMDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFAD
GVAVKRIGEETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFAEQNQLQNKQLATVLSGANTN
FHGLRYVSERCELGEKREGLLAVTIPERQGAFLEFCNIIGGRAVTEFNYRHNDESLANIFVGVRLQGGQEELEHIINDLR
EGDYAVVDLSDDEMAKLHIRYMIGGKPSKPLKERLYSFEFPEYPGALIKFLDTLGTHWNISLFNYRNHGADYGRVLCGFE
LGDDDLAQFSTHLEELGYQCKDETDNPSYKFFLS
>Mature_513_residues
SDDTSSPQKQTGADYLRQILRAPVYEAAIVTPLQDMPRLSARIGNQVQLKREDRQPVHSFKLRGAYNMVSSLSEQQKAAG
VIAASAGNHAQGMALSGSKLGIQTTIVMPKTTPDIKVDAVRGFGGNVVLHGSNFDEAKAEAERLSAEHGFTFVPPFDHPL
VIAGQGTMGMEMLQQNGHMDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADG
VAVKRIGEETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFAEQNQLQNKQLATVLSGANTNF
HGLRYVSERCELGEKREGLLAVTIPERQGAFLEFCNIIGGRAVTEFNYRHNDESLANIFVGVRLQGGQEELEHIINDLRE
GDYAVVDLSDDEMAKLHIRYMIGGKPSKPLKERLYSFEFPEYPGALIKFLDTLGTHWNISLFNYRNHGADYGRVLCGFEL
GDDDLAQFSTHLEELGYQCKDETDNPSYKFFLS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=294, Percent_Identity=34.0136054421769, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI19923959, Length=270, Percent_Identity=32.962962962963, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33469958, Length=320, Percent_Identity=26.5625, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI1790207, Length=514, Percent_Identity=69.0661478599222, Blast_Score=722, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=295, Percent_Identity=38.9830508474576, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71991565, Length=375, Percent_Identity=32.5333333333333, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=380, Percent_Identity=31.8421052631579, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17508781, Length=254, Percent_Identity=35.8267716535433, Blast_Score=145, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320930, Length=521, Percent_Identity=48.1765834932822, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322631, Length=297, Percent_Identity=37.7104377104377, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319788, Length=271, Percent_Identity=25.4612546125461, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355833, Length=375, Percent_Identity=32.5333333333333, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24645328, Length=311, Percent_Identity=29.2604501607717, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56307; Mature: 56176

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDTSSPQKQTGADYLRQILRAPVYEAAIVTPLQDMPRLSARIGNQVQLKREDRQPVHS
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCHHH
FKLRGAYNMVSSLSEQQKAAGVIAASAGNHAQGMALSGSKLGIQTTIVMPKTTPDIKVDA
HHHCHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEE
VRGFGGNVVLHGSNFDEAKAEAERLSAEHGFTFVPPFDHPLVIAGQGTMGMEMLQQNGHM
EECCCCCEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCHHHHHHHHCCCE
DYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFAD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHC
GVAVKRIGEETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFA
CHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EQNQLQNKQLATVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFLEFCNIIG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHC
GRAVTEFNYRHNDESLANIFVGVRLQGGQEELEHIINDLREGDYAVVDLSDDEMAKLHIR
CCEEEECCCCCCCHHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEE
YMIGGKPSKPLKERLYSFEFPEYPGALIKFLDTLGTHWNISLFNYRNHGADYGRVLCGFE
EEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEE
LGDDDLAQFSTHLEELGYQCKDETDNPSYKFFLS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SDDTSSPQKQTGADYLRQILRAPVYEAAIVTPLQDMPRLSARIGNQVQLKREDRQPVHS
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCHHH
FKLRGAYNMVSSLSEQQKAAGVIAASAGNHAQGMALSGSKLGIQTTIVMPKTTPDIKVDA
HHHCHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCCEEEEE
VRGFGGNVVLHGSNFDEAKAEAERLSAEHGFTFVPPFDHPLVIAGQGTMGMEMLQQNGHM
EECCCCCEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCHHHHHHHHCCCE
DYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFAD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHC
GVAVKRIGEETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFA
CHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EQNQLQNKQLATVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFLEFCNIIG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHC
GRAVTEFNYRHNDESLANIFVGVRLQGGQEELEHIINDLREGDYAVVDLSDDEMAKLHIR
CCEEEECCCCCCCHHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEE
YMIGGKPSKPLKERLYSFEFPEYPGALIKFLDTLGTHWNISLFNYRNHGADYGRVLCGFE
EEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEE
LGDDDLAQFSTHLEELGYQCKDETDNPSYKFFLS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]