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The map label for this gene is rhtC [H]

Identifier: 218710966

GI number: 218710966

Start: 3224728

End: 3225390

Strand: Direct

Name: rhtC [H]

Synonym: VS_3080

Alternate gene names: 218710966

Gene position: 3224728-3225390 (Clockwise)

Preceding gene: 218710965

Following gene: 218710967

Centisome position: 97.74

GC content: 48.11

Gene sequence:

>663_bases
ATGAACGAAGTCACCATACTGATCACCCTAGCCTCTATCCACTTCATCGCTTTAATGAGCCCCGGCCCTGACTTTGCGTT
AGTCGTGCAAAATGCGACGCGTCACGGACGCCAGACAGGCTTGTACATTGCGCTTGGCTTATCTTGCGGGATCTTGCTGC
ACTCATTGCTGAGCCTGACGGGAATCAGTTACCTAGTCCACCAGCAACCCACACTATTTGCCATTATCCAATTGGCCGGC
GGTAGCTACTTACTGTACTTAGGCTACGGTGCACTTAAAGCAACCTGGCAAATCATTCAGAACCACGACGATGACGCCGA
TACCGTTAATTCTAAAGATCTGATTCTGACCAACAAGCGAGATGCATTTTCTAAAGGGTTTATGACCAACATTCTCAACC
CAAAGGCTTTGGTGTTCTTTATTAGTTTAATGTCGAGCTTAGTCCCTGCGGATATGTCGCTGTCAGGCAAAGGCTTTGCC
CTGATCATCCTATTTGGCCTCTCACTGTTTTGGTTCTCACTGCTGGCGTGGATGCTTTCAACCAAAACACTGCAGAAGAA
ACTCAGCGAAGCGACCGTTTACATCGATGGCTTGTGTGGCGTGTTATTCAGCCTAATTGGCGCGAGTATTCTTTGGCAGT
CACTGTCCGGTTTAATCGCTTAA

Upstream 100 bases:

>100_bases
AGTGTTGATAATTTACAATTGCTCGATTTCAAATCACGGTAAGCTACGCTCAATACGATTACTTATTTAAAAATAGCGGC
AACGTTATTGAGAACTGATG

Downstream 100 bases:

>100_bases
ATTCGAATCAAGATTACAATTCGTTAACAATGCTCTGGCTATCTCATCTTTCCACTGCCAATCTAGCTCTGCATATTAAA
AGGAGAAGATCATGCAGTGG

Product: putative threonine efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNEVTILITLASIHFIALMSPGPDFALVVQNATRHGRQTGLYIALGLSCGILLHSLLSLTGISYLVHQQPTLFAIIQLAG
GSYLLYLGYGALKATWQIIQNHDDDADTVNSKDLILTNKRDAFSKGFMTNILNPKALVFFISLMSSLVPADMSLSGKGFA
LIILFGLSLFWFSLLAWMLSTKTLQKKLSEATVYIDGLCGVLFSLIGASILWQSLSGLIA

Sequences:

>Translated_220_residues
MNEVTILITLASIHFIALMSPGPDFALVVQNATRHGRQTGLYIALGLSCGILLHSLLSLTGISYLVHQQPTLFAIIQLAG
GSYLLYLGYGALKATWQIIQNHDDDADTVNSKDLILTNKRDAFSKGFMTNILNPKALVFFISLMSSLVPADMSLSGKGFA
LIILFGLSLFWFSLLAWMLSTKTLQKKLSEATVYIDGLCGVLFSLIGASILWQSLSGLIA
>Mature_220_residues
MNEVTILITLASIHFIALMSPGPDFALVVQNATRHGRQTGLYIALGLSCGILLHSLLSLTGISYLVHQQPTLFAIIQLAG
GSYLLYLGYGALKATWQIIQNHDDDADTVNSKDLILTNKRDAFSKGFMTNILNPKALVFFISLMSSLVPADMSLSGKGFA
LIILFGLSLFWFSLLAWMLSTKTLQKKLSEATVYIDGLCGVLFSLIGASILWQSLSGLIA

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=204, Percent_Identity=30.3921568627451, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786522, Length=201, Percent_Identity=21.3930348258706, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788099, Length=196, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23873; Mature: 23873

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVTILITLASIHFIALMSPGPDFALVVQNATRHGRQTGLYIALGLSCGILLHSLLSLT
CCCEEEEEEEHHHHEEEEECCCCCEEEEEECHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
GISYLVHQQPTLFAIIQLAGGSYLLYLGYGALKATWQIIQNHDDDADTVNSKDLILTNKR
HHHHHHHCCCHHHEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DAFSKGFMTNILNPKALVFFISLMSSLVPADMSLSGKGFALIILFGLSLFWFSLLAWMLS
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TKTLQKKLSEATVYIDGLCGVLFSLIGASILWQSLSGLIA
HHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEVTILITLASIHFIALMSPGPDFALVVQNATRHGRQTGLYIALGLSCGILLHSLLSLT
CCCEEEEEEEHHHHEEEEECCCCCEEEEEECHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
GISYLVHQQPTLFAIIQLAGGSYLLYLGYGALKATWQIIQNHDDDADTVNSKDLILTNKR
HHHHHHHCCCHHHEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCEEEECCC
DAFSKGFMTNILNPKALVFFISLMSSLVPADMSLSGKGFALIILFGLSLFWFSLLAWMLS
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TKTLQKKLSEATVYIDGLCGVLFSLIGASILWQSLSGLIA
HHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]