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The map label for this gene is rpoH [H]

Identifier: 218710915

GI number: 218710915

Start: 3166085

End: 3166996

Strand: Reverse

Name: rpoH [H]

Synonym: VS_3018

Alternate gene names: 218710915

Gene position: 3166996-3166085 (Counterclockwise)

Preceding gene: 218710916

Following gene: 218710914

Centisome position: 95.99

GC content: 46.49

Gene sequence:

>912_bases
TTGTCGGGTTTTAATAGATGTATTACTCCAGATCAGAGATTGATGAGGAACCGAATGGCAAACCAAGCGTATCAAATGGC
TTTAGTCACACAAGATAGTTTAGATAGCTATATCCGCTCAGCGAACAGTTACCCAATGCTGACGCCTGACGAGGAACGTG
GACTTGCAGAGCGATTACATTACAAAGGTGAGATCGACGCTGCGAAAGGCTTGATCCTTTCACACCTACGATTCGTGGTG
CACGTTGCAAGAGGCTACTCTGGCTACGGGCTGCCAATGGCTGACCTTGTCCAAGAAGGTAACATCGGCTTGATGAAGGC
TGTTAAGCGCTTTAATCCAGAAGTTGGTGTCCGTCTAGTCTCTTTTGCTGTTCACTGGATCAAAGCTGAGATTCATGAAT
ACGTATTGCGTAACTGGCGTATCGTTAAGATCGCAACGACTAAAGCACAGCGTAAATTATTCTTTAACCTTCGTAAGTCT
AAAAAGCGTTTAGGTTGGTTCAATAACGGTGAAGTTGAGACGGTTGCGCGTGAACTGGGTGTTGAGCCTTCAGAAGTTCG
TGAAATGGAATCTCGCTTAGCGGCACAAGACGCGACGTTTGAAGCGCCTATGGACGACGACGACGGTGGTTCTGCTTACA
CAGCTCCAGTTTATTACCTAGAAGATAAAGCGTCAGACGTTGCTGAGACGGTTGAGGCAGCTAACTGGGAATCTCATACT
AATAATCGCCTAGGTCTCGCATTGAAGAGCTTAGACGAGCGTAGTCAACACATTGTTCGCTCACGTTGGTTAGACGACAA
CAAAGCAACACTGCAAGACTTAGCGGATACCTATAGCATTTCTGCAGAGCGTGTCCGCCAGTTAGAAAAGAATGCGATGA
AGAAATTGAAGCAAGCCGTTGGCGACTTCTAA

Upstream 100 bases:

>100_bases
AAAAATAGGCAGTTCTTAACCAGTTTTTGCCTTGTATAGACGAATTGATTATGCATAATTACTTCCCCCTCCTTGAAACC
TGTTCATTTTAGGTTCAAGA

Downstream 100 bases:

>100_bases
TAGCATTTAATTTTTAATAGTATTTGGTTTCGCATAGTAGCAAGCAAATAGCTTAATTTTGAAAAGGCCGAGATCGAGAG
ATCTCGGCCTTTTTGTTTTG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSGFNRCITPDQRLMRNRMANQAYQMALVTQDSLDSYIRSANSYPMLTPDEERGLAERLHYKGEIDAAKGLILSHLRFVV
HVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKS
KKRLGWFNNGEVETVARELGVEPSEVREMESRLAAQDATFEAPMDDDDGGSAYTAPVYYLEDKASDVAETVEAANWESHT
NNRLGLALKSLDERSQHIVRSRWLDDNKATLQDLADTYSISAERVRQLEKNAMKKLKQAVGDF

Sequences:

>Translated_303_residues
MSGFNRCITPDQRLMRNRMANQAYQMALVTQDSLDSYIRSANSYPMLTPDEERGLAERLHYKGEIDAAKGLILSHLRFVV
HVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKS
KKRLGWFNNGEVETVARELGVEPSEVREMESRLAAQDATFEAPMDDDDGGSAYTAPVYYLEDKASDVAETVEAANWESHT
NNRLGLALKSLDERSQHIVRSRWLDDNKATLQDLADTYSISAERVRQLEKNAMKKLKQAVGDF
>Mature_302_residues
SGFNRCITPDQRLMRNRMANQAYQMALVTQDSLDSYIRSANSYPMLTPDEERGLAERLHYKGEIDAAKGLILSHLRFVVH
VARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSK
KRLGWFNNGEVETVARELGVEPSEVREMESRLAAQDATFEAPMDDDDGGSAYTAPVYYLEDKASDVAETVEAANWESHTN
NRLGLALKSLDERSQHIVRSRWLDDNKATLQDLADTYSISAERVRQLEKNAMKKLKQAVGDF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=70.3180212014134, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1789098, Length=290, Percent_Identity=26.2068965517241, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=27.0042194092827, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34459; Mature: 34327

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFNRCITPDQRLMRNRMANQAYQMALVTQDSLDSYIRSANSYPMLTPDEERGLAERLH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
YKGEIDAAKGLILSHLRFVVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHH
SFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELG
HHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
VEPSEVREMESRLAAQDATFEAPMDDDDGGSAYTAPVYYLEDKASDVAETVEAANWESHT
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECEEEEECCHHHHHHHHHHHCCCCCCC
NNRLGLALKSLDERSQHIVRSRWLDDNKATLQDLADTYSISAERVRQLEKNAMKKLKQAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GDF
CCC
>Mature Secondary Structure 
SGFNRCITPDQRLMRNRMANQAYQMALVTQDSLDSYIRSANSYPMLTPDEERGLAERLH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
YKGEIDAAKGLILSHLRFVVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEVGVRLV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHH
SFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELG
HHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
VEPSEVREMESRLAAQDATFEAPMDDDDGGSAYTAPVYYLEDKASDVAETVEAANWESHT
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECEEEEECCHHHHHHHHHHHCCCCCCC
NNRLGLALKSLDERSQHIVRSRWLDDNKATLQDLADTYSISAERVRQLEKNAMKKLKQAV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GDF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA