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The map label for this gene is glpE

Identifier: 218710914

GI number: 218710914

Start: 3165323

End: 3165643

Strand: Reverse

Name: glpE

Synonym: VS_3017

Alternate gene names: 218710914

Gene position: 3165643-3165323 (Counterclockwise)

Preceding gene: 218710915

Following gene: 218710913

Centisome position: 95.95

GC content: 45.48

Gene sequence:

>321_bases
ATGGACCAGTTTAAACATATCGACGTTAAAGGCGCTCAAGCCCTTATTGAACAAAATGAAGCTCGACTTGTCGATATACG
AGATCCGCAATCTTTTGCGGTAGCCCATTCAGAAACCGCCTACCACCTGACTAACGATACCATGGTGTCGTTTATGGATG
AGGTTGAGTTCGAACAGCCTATCTTAGTGATGTGTTACCACGGTATTAGTAGCCAAGGCGCGGCACAATATTTAGTGAAC
CAAGGCTTTGAAGAGGTATATAGTGTTGATGGCGGTTTCGAAGCTTCGTATCGTGCTGAACTTCCGGTGATTGCGGGTTA
A

Upstream 100 bases:

>100_bases
AAGTGATCGCTGGTGGATAACCTTATTAAGTGGGTGTGATTGTTAGCCTTGGGATAGGTTACAATGGCAGCAAAGTAAAT
CAGTATAAAAAGAGAAAGTC

Downstream 100 bases:

>100_bases
TAGCTTTATCACTTAAACGCTTCAAGCATCTCCATAGTATGAATAGGAAGTAACAATGATTAGATTGATGGTGTTAGACA
ACCCACGTCTTGCTCAAGCA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MDQFKHIDVKGAQALIEQNEARLVDIRDPQSFAVAHSETAYHLTNDTMVSFMDEVEFEQPILVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEASYRAELPVIAG

Sequences:

>Translated_106_residues
MDQFKHIDVKGAQALIEQNEARLVDIRDPQSFAVAHSETAYHLTNDTMVSFMDEVEFEQPILVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEASYRAELPVIAG
>Mature_106_residues
MDQFKHIDVKGAQALIEQNEARLVDIRDPQSFAVAHSETAYHLTNDTMVSFMDEVEFEQPILVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEASYRAELPVIAG

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1789831, Length=98, Percent_Identity=59.1836734693878, Blast_Score=127, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPE_VIBSL (B7VMA4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418535.1
- ProteinModelPortal:   B7VMA4
- GeneID:   7162481
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3017
- HOGENOM:   HBG743107
- OMA:   FSAWHEA
- ProtClustDB:   PRK00162
- GO:   GO:0005737
- HAMAP:   MF_01009
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 11817; Mature: 11817

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFKHIDVKGAQALIEQNEARLVDIRDPQSFAVAHSETAYHLTNDTMVSFMDEVEFEQP
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHCCCCCC
ILVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEASYRAELPVIAG
EEEEEECCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MDQFKHIDVKGAQALIEQNEARLVDIRDPQSFAVAHSETAYHLTNDTMVSFMDEVEFEQP
CCCCCCCCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHCCCCCC
ILVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEASYRAELPVIAG
EEEEEECCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA