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The map label for this gene is birA [H]

Identifier: 218710889

GI number: 218710889

Start: 3135740

End: 3136705

Strand: Reverse

Name: birA [H]

Synonym: VS_2979

Alternate gene names: 218710889

Gene position: 3136705-3135740 (Counterclockwise)

Preceding gene: 218710890

Following gene: 218710887

Centisome position: 95.07

GC content: 45.45

Gene sequence:

>966_bases
ATGAGAGAACACAGTACCAAGCTTGCACTATTGAGATGTCTTGCTGACGGTGAGTTTCATTCAGGTGAAGCTCTAGGTGA
AATGATTGGTGTATCACGAGCGGCTATCAGCAAGCATATTAAAGGTATTCAAGACTGGGGGTTAGATATCTACCGAGTTC
AAGGCAAGGGCTATAAGCTAGCTAGCCGTTTAGATATGCTTGACGAAGACAAATTGTCTGTTGTTAGTCGTGATGCTTCT
CTTGAACTCATTCCAATCATAGGTTCAACAAACCAGCACCTATTAGAGCGTACTAACATCCTCGAATCGGGTTCTGTCTG
TATTGCTGAATATCAAGCTGCGGGACGTGGACGCCGTGGACGAGAGTGGGTATCGCCATTCGGTGCAAACCTCTATCTTT
CAATGTATTGGAGACTCGACGCTGGGATGGCTGCCGCGATGGGCTTGAGCCTTGTGGTTGGTGTAGCTGTTGTTGAAGCT
TTGGAAGAGATGGGCGTAGAAGGTATAAAGCTCAAATGGCCGAACGACCTTTACCACAATGACAAGAAGCTTGCTGGTAT
CTTGGTAGAGCTGTCAGGACAGTCTGGTGGCGCTGCTCATATTGTGATTGGTTTAGGGCTAAACTTATCCATGGATCCAA
CAACATCAGGTATTGGCCAACCGTGGACTTCTCTTAAAGAAGTGTGTGATGGGAAGGTGCCCGATCGTAATCAGTTAGCA
CAGGCTCTGATCAATGCCTGGGATAAGTCTCTTGTTGATTATGAGTTGAAAGGGATGTCCAATTTTGTAGAGCGTTGGAA
TCGCTTTGATAATTTCTTAGGTCGCAATGTTAGGTTGATCATTGGGCCTAGAGAAATTGAAGGTGTTGTTCAAGGCATCG
ATGAACAAGGTGCTGTATTACTCAAAACTGAGAATGGTATCGAGAGCTATATCGGCGGAGAGATATCGCTAAGAAAAGGC
GATTAA

Upstream 100 bases:

>100_bases
AGTCTGTCTTGAATAAATTCGATATTCGACTGGAACATGAAGTACGTTTTATGGGACGAGACTGCGAGACAAACCTAGAC
AAGGCTTTGGAGTCATTGGC

Downstream 100 bases:

>100_bases
GGTGTTACTATTTTCTTAATAACACTTCTTCAACCATATGATCTGCGCCTTTACGTAGAATCAAGTGAGCCCTTTCTCGT
GTAGGAAGGATATTTTGTTC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MREHSTKLALLRCLADGEFHSGEALGEMIGVSRAAISKHIKGIQDWGLDIYRVQGKGYKLASRLDMLDEDKLSVVSRDAS
LELIPIIGSTNQHLLERTNILESGSVCIAEYQAAGRGRRGREWVSPFGANLYLSMYWRLDAGMAAAMGLSLVVGVAVVEA
LEEMGVEGIKLKWPNDLYHNDKKLAGILVELSGQSGGAAHIVIGLGLNLSMDPTTSGIGQPWTSLKEVCDGKVPDRNQLA
QALINAWDKSLVDYELKGMSNFVERWNRFDNFLGRNVRLIIGPREIEGVVQGIDEQGAVLLKTENGIESYIGGEISLRKG
D

Sequences:

>Translated_321_residues
MREHSTKLALLRCLADGEFHSGEALGEMIGVSRAAISKHIKGIQDWGLDIYRVQGKGYKLASRLDMLDEDKLSVVSRDAS
LELIPIIGSTNQHLLERTNILESGSVCIAEYQAAGRGRRGREWVSPFGANLYLSMYWRLDAGMAAAMGLSLVVGVAVVEA
LEEMGVEGIKLKWPNDLYHNDKKLAGILVELSGQSGGAAHIVIGLGLNLSMDPTTSGIGQPWTSLKEVCDGKVPDRNQLA
QALINAWDKSLVDYELKGMSNFVERWNRFDNFLGRNVRLIIGPREIEGVVQGIDEQGAVLLKTENGIESYIGGEISLRKG
D
>Mature_321_residues
MREHSTKLALLRCLADGEFHSGEALGEMIGVSRAAISKHIKGIQDWGLDIYRVQGKGYKLASRLDMLDEDKLSVVSRDAS
LELIPIIGSTNQHLLERTNILESGSVCIAEYQAAGRGRRGREWVSPFGANLYLSMYWRLDAGMAAAMGLSLVVGVAVVEA
LEEMGVEGIKLKWPNDLYHNDKKLAGILVELSGQSGGAAHIVIGLGLNLSMDPTTSGIGQPWTSLKEVCDGKVPDRNQLA
QALINAWDKSLVDYELKGMSNFVERWNRFDNFLGRNVRLIIGPREIEGVVQGIDEQGAVLLKTENGIESYIGGEISLRKG
D

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=216, Percent_Identity=28.7037037037037, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790408, Length=322, Percent_Identity=50.9316770186335, Blast_Score=332, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32563944, Length=230, Percent_Identity=25.6521739130435, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32563942, Length=230, Percent_Identity=25.6521739130435, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644246, Length=270, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35091; Mature: 35091

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREHSTKLALLRCLADGEFHSGEALGEMIGVSRAAISKHIKGIQDWGLDIYRVQGKGYKL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEEH
ASRLDMLDEDKLSVVSRDASLELIPIIGSTNQHLLERTNILESGSVCIAEYQAAGRGRRG
HHHHCCCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCC
REWVSPFGANLYLSMYWRLDAGMAAAMGLSLVVGVAVVEALEEMGVEGIKLKWPNDLYHN
CHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCC
DKKLAGILVELSGQSGGAAHIVIGLGLNLSMDPTTSGIGQPWTSLKEVCDGKVPDRNQLA
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHH
QALINAWDKSLVDYELKGMSNFVERWNRFDNFLGRNVRLIIGPREIEGVVQGIDEQGAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEE
LKTENGIESYIGGEISLRKGD
EEECCCHHHHCCCEEEEECCC
>Mature Secondary Structure
MREHSTKLALLRCLADGEFHSGEALGEMIGVSRAAISKHIKGIQDWGLDIYRVQGKGYKL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEEH
ASRLDMLDEDKLSVVSRDASLELIPIIGSTNQHLLERTNILESGSVCIAEYQAAGRGRRG
HHHHCCCCCCHHHHEECCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCC
REWVSPFGANLYLSMYWRLDAGMAAAMGLSLVVGVAVVEALEEMGVEGIKLKWPNDLYHN
CHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHCC
DKKLAGILVELSGQSGGAAHIVIGLGLNLSMDPTTSGIGQPWTSLKEVCDGKVPDRNQLA
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHH
QALINAWDKSLVDYELKGMSNFVERWNRFDNFLGRNVRLIIGPREIEGVVQGIDEQGAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEE
LKTENGIESYIGGEISLRKGD
EEECCCHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]