Click here to switch to the map view.

The map label for this gene is tuf1 [H]

Identifier: 218710887

GI number: 218710887

Start: 3132912

End: 3134150

Strand: Reverse

Name: tuf1 [H]

Synonym: VS_2972

Alternate gene names: 218710887

Gene position: 3134150-3132912 (Counterclockwise)

Preceding gene: 218710889

Following gene: 218710886

Centisome position: 94.99

GC content: 46.49

Gene sequence:

>1239_bases
TTGGTTGCGTGGTCTTATTTTAAGCCACCTAATCCGTACCTAGAGGGACACCCCATGTCTAAAGAAAAATTTGAACGTAC
GAAACCGCACGTAAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACAACTCTAACTGCTGCTATCTGTACTACAC
TTGCAAAAGTGTACGGCGGTGTTGCTAAAGATTTCGCATCTATCGATAACGCTCCAGAAGAGCGCGAGCGCGGTATCACA
ATCGCAACTTCTCACGTTGAGTACGATACTCCTGAACGTCACTACGCACACGTAGACTGTCCTGGACACGCCGATTATGT
TAAAAACATGATCACTGGTGCTGCTCAAATGGACGGCGGTATCCTAGTTGTTGCTGCTACAGATGGCCCTATGCCACAAA
CTCGTGAGCACATCCTACTTGGTCGTCAAGTTGGTATCCCTTACATCATCGTATTCATGAACAAATGTGACATGGTTGAT
GACGAAGAGCTACTTGAGCTAGTAGAAATGGAAGTTCGTGAACTTCTTTCTGAGTACGAGTACCCAGGAGACGACCTACC
AGTAATTCAAGGTTCTGCACTTGGCGCTCTAAACGGCGAAAAGCAGTGGGAAGACAAGATCGTTGAGCTTGCAGAAGCAC
TAGATTCTTACATTCCACTTCCAGAGCGTGCAGTTGATCTACCGTTCCTACTTCCTATTGAAGATGTATTCTCAATCCAA
GGTCGTGGTACTGTAGTTACTGGTCGTATCGAGCGCGGTATCCTACGTGTAGGTGACGAAGTAGAAATCGTTGGTATCAA
AGAGACTACTCTTACTACTTGTACTGGTGTTGAAATGTTCCGTAAACTGCTTGACGAAGGTCGTGCAGGTGAGAACGTTG
GTGCACTTCTACGTGGTACTAAGCGTGATGACGTTGAACGTGGCCAAGTACTTTCTGCTAAAGGTTCAATCAACCCACAC
ACTAAGTTTGAGTCTGAAGTATACGTACTTTCTAAAGACGAAGGCGGCCGTCACACTCCTTTCTTCAAGGGTTACCGTCC
ACAGTTCTACTTCCGTACAACTGACGTAACAGGCGATATCACTCTACCTGAAGGCGTAGAAATGGTAATGCCAGGTGACA
ACGTTCAAATGACTGTTGAGCTAATCGCTCCAATCGCAATGGACGAAGGTCTACGTTTCGCAATCCGCGAAGGTGGCCGT
ACAGTTGGTGCTGGTGTTGTAGCTAAAATCTTTGCATAA

Upstream 100 bases:

>100_bases
GGTAGAGCGCACCCTTGGTAAGGGTGAGGTCCCCAGTTCGACTCTGGGTATTAGCACCAGTCAAAAGCTTCTTCTCCTTT
AATAAAGAAAACCATTTTTT

Downstream 100 bases:

>100_bases
GATTTGACGAACCACTAGTAAAAAGGGCATCATTTGATGCCCTTTTTCTGCGCTGAAAAAAAGAGTTGGGTGTTTTGACA
TCAATTTTAACTCTTAGCAA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MVAWSYFKPPNPYLEGHPMSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGIT
IATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVD
DEELLELVEMEVRELLSEYEYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQ
GRGTVVTGRIERGILRVGDEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPH
TKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGR
TVGAGVVAKIFA

Sequences:

>Translated_412_residues
MVAWSYFKPPNPYLEGHPMSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGIT
IATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVD
DEELLELVEMEVRELLSEYEYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQ
GRGTVVTGRIERGILRVGDEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPH
TKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGR
TVGAGVVAKIFA
>Mature_412_residues
MVAWSYFKPPNPYLEGHPMSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGIT
IATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVD
DEELLELVEMEVRELLSEYEYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQ
GRGTVVTGRIERGILRVGDEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPH
TKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGR
TVGAGVVAKIFA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.1898734177215, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.7727272727273, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.7727272727273, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=454, Percent_Identity=30.1762114537445, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.47533632287, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI54607086, Length=311, Percent_Identity=28.9389067524116, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=331, Percent_Identity=29.607250755287, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194018522, Length=331, Percent_Identity=29.607250755287, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194018520, Length=331, Percent_Identity=29.607250755287, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI46094014, Length=321, Percent_Identity=29.9065420560748, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=32.5757575757576, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=83.756345177665, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=83.756345177665, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=29.0575916230367, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789108, Length=327, Percent_Identity=29.0519877675841, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50.4975124378109, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.702842377261, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=331, Percent_Identity=33.2326283987915, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=331, Percent_Identity=33.2326283987915, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.6887871853547, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=27.1889400921659, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=313, Percent_Identity=31.3099041533546, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=25.1101321585903, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17506081, Length=242, Percent_Identity=30.5785123966942, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17509919, Length=455, Percent_Identity=27.2527472527473, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=151, Percent_Identity=32.4503311258278, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.5106382978723, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=61.7721518987342, Blast_Score=501, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.0909090909091, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.0909090909091, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=384, Percent_Identity=24.21875, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=303, Percent_Identity=26.7326732673267, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=29.2181069958848, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=55.4156171284635, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=55.4156171284635, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=413, Percent_Identity=49.636803874092, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.1938325991189, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.1938325991189, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.1390728476821, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=26.4367816091954, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.2753623188406, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=27.9569892473118, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=27.9569892473118, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=245, Percent_Identity=31.8367346938775, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 45348; Mature: 45348

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAWSYFKPPNPYLEGHPMSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGG
CCEECCCCCCCCCCCCCCCCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHH
VAKDFASIDNAPEERERGITIATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGG
HHHHHHHCCCCCHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCC
ILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYE
EEEEEECCCCCCCCHHHHEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
YPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQ
CCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECHHHHHCCC
GRGTVVTGRIERGILRVGDEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGT
CCCEEEEEHHHCCCEECCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHCCC
KRDDVERGQVLSAKGSINPHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDI
CCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCE
TLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
ECCCCCEEEECCCCCEEEEEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure
MVAWSYFKPPNPYLEGHPMSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGG
CCEECCCCCCCCCCCCCCCCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHH
VAKDFASIDNAPEERERGITIATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGG
HHHHHHHCCCCCHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCC
ILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYE
EEEEEECCCCCCCCHHHHEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
YPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQ
CCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECHHHHHCCC
GRGTVVTGRIERGILRVGDEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGT
CCCEEEEEHHHCCCEECCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHCCC
KRDDVERGQVLSAKGSINPHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDI
CCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCE
TLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
ECCCCCEEEECCCCCEEEEEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA