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The map label for this gene is mlaE [H]

Identifier: 218710669

GI number: 218710669

Start: 2914805

End: 2915590

Strand: Reverse

Name: mlaE [H]

Synonym: VS_2749

Alternate gene names: 218710669

Gene position: 2915590-2914805 (Counterclockwise)

Preceding gene: 218710670

Following gene: 218710668

Centisome position: 88.37

GC content: 46.44

Gene sequence:

>786_bases
TTGAATATGATTGCTAAAACTATTGCGGGTGTCGGTAAGCGTGCACTTGCGATCTGTGAGTCATTTGGTCGAGCAAGCCT
AATGCTATTTGGGGCTTTGTTTGGCATCCCGAAACTAAAAAACTTCCCTCTATTAGTTAAACAATTTTATAGCGTTGGCG
TTCAGTCATTGGCTATCATTTTAGTTTCGGGCTTGTTTATTGGCATGGTACTCAGTCTGCAGGGTTATGTCGTATTAATC
GACTATGGCGCAGAGGGTAATCTTGGGCAAATGGTCGCGCTCTCGTTGTTACGGGAATTGGGTCCTGTGGTGACAGCACT
TCTGTTTGCTGGCCGTGCGGGTTCAGCATTAACGGCTGAGATCGGCTTAATGAAAGCAACGGAACAGATCTCTAGCCTTG
AGATGATGGCGGTTGATCCTCTTAAACGCATTATTGCGCCACGTCTCTGGGCCGGCCTTATTTCTATGCCACTGCTCGCT
ATGATTTTTATGGCGGTCGGGATTTGGGGTGCTCAGTTAGTCGGTGTGGATTGGAAAGGTATTGACCACGGCAGTTTCTG
GTCTGCGATGCAGTCTTCCGTTGAGCTTGGCCGTGATATTGGTAACAGTATGATCAAATGCATGGTCTTCGCTATCACCG
TAACTTGGATCGCACTTTTTAATGGTTATGATGCAGTACCGACATCCGAAGGTATTAGTCAGGCAACCACACGCACAGTA
GTACACTCTTCTCTAGCGGTATTAGGGCTAGATTTTGTTCTTACCGCATTGATGTTTGGGAATTAA

Upstream 100 bases:

>100_bases
TACAACAACAGCGACCCAAGAGTGCAGCAATTTTTGCAAGGTGAATCGGATGGCCCTGTGCCATTTAGGTTTCCTGCAAA
TAGCTTAGAAAAGGATTTGT

Downstream 100 bases:

>100_bases
TCATGCAACAAACTCGAAAATTAGAATTATGGGCCGGCACCTTTGTTATTGCCGGAATTTGCGCAATCTTAATCATGATC
TTTCAAGTCGCTGACGTAAA

Product: ABC transport protein: transmembrane protein (IM)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNMIAKTIAGVGKRALAICESFGRASLMLFGALFGIPKLKNFPLLVKQFYSVGVQSLAIILVSGLFIGMVLSLQGYVVLI
DYGAEGNLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLKRIIAPRLWAGLISMPLLA
MIFMAVGIWGAQLVGVDWKGIDHGSFWSAMQSSVELGRDIGNSMIKCMVFAITVTWIALFNGYDAVPTSEGISQATTRTV
VHSSLAVLGLDFVLTALMFGN

Sequences:

>Translated_261_residues
MNMIAKTIAGVGKRALAICESFGRASLMLFGALFGIPKLKNFPLLVKQFYSVGVQSLAIILVSGLFIGMVLSLQGYVVLI
DYGAEGNLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLKRIIAPRLWAGLISMPLLA
MIFMAVGIWGAQLVGVDWKGIDHGSFWSAMQSSVELGRDIGNSMIKCMVFAITVTWIALFNGYDAVPTSEGISQATTRTV
VHSSLAVLGLDFVLTALMFGN
>Mature_261_residues
MNMIAKTIAGVGKRALAICESFGRASLMLFGALFGIPKLKNFPLLVKQFYSVGVQSLAIILVSGLFIGMVLSLQGYVVLI
DYGAEGNLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAVDPLKRIIAPRLWAGLISMPLLA
MIFMAVGIWGAQLVGVDWKGIDHGSFWSAMQSSVELGRDIGNSMIKCMVFAITVTWIALFNGYDAVPTSEGISQATTRTV
VHSSLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=70.7692307692308, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27767; Mature: 27767

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMIAKTIAGVGKRALAICESFGRASLMLFGALFGIPKLKNFPLLVKQFYSVGVQSLAII
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLSLQGYVVLIDYGAEGNLGQMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKATEQISSLEMMAVDPLKRIIAPRLWAGLISMPLLAMIFMAVGIWGAQLVGVDWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
IDHGSFWSAMQSSVELGRDIGNSMIKCMVFAITVTWIALFNGYDAVPTSEGISQATTRTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VHSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNMIAKTIAGVGKRALAICESFGRASLMLFGALFGIPKLKNFPLLVKQFYSVGVQSLAII
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLSLQGYVVLIDYGAEGNLGQMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKATEQISSLEMMAVDPLKRIIAPRLWAGLISMPLLAMIFMAVGIWGAQLVGVDWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
IDHGSFWSAMQSSVELGRDIGNSMIKCMVFAITVTWIALFNGYDAVPTSEGISQATTRTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VHSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]