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The map label for this gene is pilT [H]

Identifier: 218710628

GI number: 218710628

Start: 2870342

End: 2871379

Strand: Reverse

Name: pilT [H]

Synonym: VS_2682

Alternate gene names: 218710628

Gene position: 2871379-2870342 (Counterclockwise)

Preceding gene: 218710636

Following gene: 218710627

Centisome position: 87.03

GC content: 45.18

Gene sequence:

>1038_bases
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCCGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGGACGACTCACAGCGCGGTGAGTTTGAGGAGAAATTGGAAGTAGACTTCTCTTTTGAATTACCCAATGTTGGTCGA
TTCCGTGTTAATGCGTTTAACCAAGCTCGTGGGTGCGCAGCGGTCTTTCGAACCATCCCTGTAGAAATCCCAACGTTAGA
GCAGTTGGGCGCACCTGATATTTTTGAAAAAATTGCCAATTATGAAAAAGGCTTAGTGCTGGTGACTGGCCCTACGGGGT
CTGGTAAATCGACAACTTTGGCGGCGATGGTTGATTATGTAAACCGTAATCACAACAAACACATTTTGACCATTGAAGAC
CCAATTGAATTCGTTCACACCAATAATAAATGTCTGGTTAACCAGCGTGAAGTTCATCGTGATACGCACAGCTTTAAAGC
GGCGCTACGCAGTGCGTTACGTGAAGACCCGGACGTAATTCTTGTAGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACGGCCGCAGAAACTGGTCACCTAGTTTTTGGTACTTTGCATACTAGCTCTGCAGCAAAAACCGTAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGATATGGTTCGTTCGATGCTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
ACTATTAAAGCGTGTGGGTGGTGGTCGTGTCGCTTGTCATGAAATCATGATGGCGACACCTGCAATCAGAAACTTGATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATTATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAACGCG
AAACAACTGATGGCACAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATTGAAATTGAAACCTCAATGTTTTAA

Upstream 100 bases:

>100_bases
GCTCGTCCGCACTTTTGTTCAGCACTACGAATCTGTGAGGTGATTTGTTCGATATTTTGTTGAATACTACTCATAGCTGA
CTTTACTTAAGGGAAAATAA

Downstream 100 bases:

>100_bases
TCAAGGTGAACAAAATGGAATTGAATCAAATTCTTGAAGGGATGCTCTCTCAAAAGGCGTCGGATCTTTATATCACTGTC
GATGCGCCAATCCTTTTTCG

Product: Tfp pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Sequences:

>Translated_345_residues
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF
>Mature_345_residues
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=48.3180428134557, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1789723, Length=350, Percent_Identity=33.4285714285714, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=30.2872062663185, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38228; Mature: 38228

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GEFEEKLEVDFSFELPNVGRFRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIAN
CCCHHHEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHC
YEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIEDPIEFVHTNNKCLVNQREVHR
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEEECHHHHHH
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHH
MYSIIQTGAAHGMQTMEQNAKQLMAQGLVDSEEVEKKIEIETSMF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GEFEEKLEVDFSFELPNVGRFRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIAN
CCCHHHEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHC
YEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIEDPIEFVHTNNKCLVNQREVHR
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEEECHHHHHH
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHH
MYSIIQTGAAHGMQTMEQNAKQLMAQGLVDSEEVEKKIEIETSMF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]