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The map label for this gene is pilT [H]

Identifier: 218710627

GI number: 218710627

Start: 2869218

End: 2870336

Strand: Reverse

Name: pilT [H]

Synonym: VS_2681

Alternate gene names: 218710627

Gene position: 2870336-2869218 (Counterclockwise)

Preceding gene: 218710628

Following gene: 218710622

Centisome position: 87.0

GC content: 46.47

Gene sequence:

>1119_bases
GTGAACAAAATGGAATTGAATCAAATTCTTGAAGGGATGCTCTCTCAAAAGGCGTCGGATCTTTATATCACTGTCGATGC
GCCAATCCTTTTTCGTGTGGATGGTGAACTACGACCTCAGGGAGAGAAGTTGAATGCTGATCAAGTATCTCAGTTACTTG
ATGCAATGATGGATCAAGATCGACGCGATGAATACAAGCAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGT
CGTTTTCGTGTCAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTAATTCGTCGCATCGAGACTAACATCCCAACCTT
TGAGCAATTAAAGCTTCCTGACGTACTACAAGACCTTTCAATCGCTAAGCGCGGGCTTGTGCTGGTGGTTGGTGCGACTG
GCTCTGGTAAATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCTTGACGGTTGAA
GACCCGATTGAATTCGTACACGAACATAAAAAGTGCATCGTGACTCAACGAGAGGTTGGACTCGACACCGAAAGCTATGA
AGTCGCGCTTAAAAACTCGTTACGCCAAGCCCCTGATATGATTTTGATTGGCGAAATCCGTAGTCGTGAAACCATGGAAT
ACGCGATGACCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCCTGCATGCCAATAACGCTAACCAGGCGCTAGAGCGT
ATTCTTCACTTGGTGCCGAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTCGCTCA
GCAGTTAATACGCGATAAAAATGGCAATGGTCGTCATGGTGTCTTCGAAATTCTGCTGAATAGCCCTCGTATATCTGACT
TGATTCGTCGTGGTGAGTTGCATGAGTTAAAAGCGACCATGGCCAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAA
GCTTTGTATGATTTAGTGGTTGCGGACAAGATTAGCGAGGATGATGCATTCCATAGTGCAGATTCTGCCAATGACTTGCG
TTTAATGCTCAAGACCAAACGTGGCGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTTAAGATTGATATGGGCTAA

Upstream 100 bases:

>100_bases
CCATGGAGCAAAACGCGAAACAACTGATGGCACAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATTGAAATTGAA
ACCTCAATGTTTTAATCAAG

Downstream 100 bases:

>100_bases
GTCACGGCTTTGTTGATAAACACAAAACCCGCTTCTGTGGCGGGTCTTATTCGTTTATAACTTAATGCATGTTAGATGAG
TGCATGTTGGAGCGATGGTT

Product: Tfp pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNKMELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFG
RFRVSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVE
DPIEFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALER
ILHLVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQ
ALYDLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Sequences:

>Translated_372_residues
MNKMELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFG
RFRVSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVE
DPIEFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALER
ILHLVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQ
ALYDLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG
>Mature_372_residues
MNKMELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFG
RFRVSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVE
DPIEFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALER
ILHLVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQ
ALYDLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=38.5579937304075, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789723, Length=282, Percent_Identity=34.3971631205674, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786296, Length=287, Percent_Identity=30.6620209059233, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41828; Mature: 41828

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQD
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCH
RRDEYKQTREANFAIVRDFGRFRVSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLS
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHH
IAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPIEFVHEHKKCIVTQREVG
HHCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHEEEHHHHC
LDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALER
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
ILHLVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGEL
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHCCCH
HELKATMAKSKEIGMQTFDQALYDLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
TGALAGVKIDMG
CCEEEEEEEECC
>Mature Secondary Structure
MNKMELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQD
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCH
RRDEYKQTREANFAIVRDFGRFRVSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLS
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHH
IAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPIEFVHEHKKCIVTQREVG
HHCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHEEEHHHHC
LDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALER
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
ILHLVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGEL
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHCCCH
HELKATMAKSKEIGMQTFDQALYDLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
TGALAGVKIDMG
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]