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The map label for this gene is gshB [H]

Identifier: 218710624

GI number: 218710624

Start: 2866775

End: 2867725

Strand: Direct

Name: gshB [H]

Synonym: VS_2678

Alternate gene names: 218710624

Gene position: 2866775-2867725 (Clockwise)

Preceding gene: 218710623

Following gene: 218710625

Centisome position: 86.89

GC content: 45.01

Gene sequence:

>951_bases
ATGATCAAACTTGGCATCGTAATGGATCCAATTTCATCCATTAACATCAAAAAAGACTCTAGCTTTGCCATGATGCTTGA
AGCTCAGCGTCGTGGTTACGAAATCCATTACATGGAAATGGATGATCTACATTTAGATCAAGGCGTAGCCATTGCTGACA
CTAAGGTTGTTGAACTAAAAGAAGATCCAAACGGTTGGTACGAGTTCAAATCAGAACAGACTATCGCGCTATCCGATTTA
GATGCGGTACTGATGCGTAAAGATCCTCCGTTTGATACTGAGTACATCTACGCGACTTACATTCTTGAGCGTGCTGAAGA
GAACGGCGCACTGATCGTAAACAAGCCACAAAGCTTACGTGACTGTAACGAGAAGTTGTTCACGGCATGGTTCCCTGAAC
TCACACCAACCACCATCGTGACTCGTAAAGCTGAAAAGATTAAAGCGTTCCGAGAAGAGCACGGTGACGTGATCCTAAAA
CCACTTGATGGCATGGGCGGCGCGTCTATCTTCCGAGTAAAAGAAGGCGATCCAAACGTATCAGTGATCATTGAGACTTT
AACTAACCACGGTCAAAACTACGCAATGGCACAAACCTTTGTTCCAGACATCAGCAATGGTGATAAGCGTATTCTTGTGG
TTGATGGTGAACCAATGCCTTACTGCCTAGCGCGTATTCCTGCTAAAGGGGAAACTCGAGGTAACCTTGCTGCTGGTGGT
ACAGGTGAAGCTCGTCCGCTAAGTGAAACCGACTGGGCTATCGCAAGAGCAGTTGCACCGGCATTAAAAGAGAAAGGTTT
AATCTTTGTAGGACTTGATGTTATCGGTGACAAGCTGACTGAAATCAACGTGACAAGCCCTACTTGTATCCGTGAAATTG
AAGCTGCTTTTGATATTTCGGTAACGGGCAAGTTAATGGATGCAATCGAGCGTCGCATTAACGCTAAATAG

Upstream 100 bases:

>100_bases
GACGCTACTCGGTCCACGTGTACTTCGTACCGAAACTGCTGCTCTAACCGCAATTACTGCCTTACAAGTCCGTTTTGGCG
ATCTAGGCTAGGAGAAAGAA

Downstream 100 bases:

>100_bases
CCTAAACTAAAGAAAGCCTTAGCTATTCGAAAACACAAAAATATCTAGGCAAAGAACAATGAGCGGCTTTACGCCGCTCT
TTTTCCTTTACGGGGAACAC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGYEIHYMEMDDLHLDQGVAIADTKVVELKEDPNGWYEFKSEQTIALSDL
DAVLMRKDPPFDTEYIYATYILERAEENGALIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKEGDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIARAVAPALKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISVTGKLMDAIERRINAK

Sequences:

>Translated_316_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGYEIHYMEMDDLHLDQGVAIADTKVVELKEDPNGWYEFKSEQTIALSDL
DAVLMRKDPPFDTEYIYATYILERAEENGALIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKEGDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIARAVAPALKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISVTGKLMDAIERRINAK
>Mature_316_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGYEIHYMEMDDLHLDQGVAIADTKVVELKEDPNGWYEFKSEQTIALSDL
DAVLMRKDPPFDTEYIYATYILERAEENGALIVNKPQSLRDCNEKLFTAWFPELTPTTIVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKEGDPNVSVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIARAVAPALKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDISVTGKLMDAIERRINAK

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=73.1012658227848, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35035; Mature: 35035

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGYEIHYMEMDDLHLDQGVAIADTKVVELK
CEEEEEEECCCCCCEECCCCCEEEEEEHHHCCCEEEEEEECCEECCCCEEEECEEEEEEE
EDPNGWYEFKSEQTIALSDLDAVLMRKDPPFDTEYIYATYILERAEENGALIVNKPQSLR
CCCCCCEEECCCCEEEECCHHHHEECCCCCCCCCCEEEEEHHHHHCCCCEEEEECCHHHH
DCNEKLFTAWFPELTPTTIVTRKAEKIKAFREEHGDVILKPLDGMGGASIFRVKEGDPNV
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEEECC
TGEARPLSETDWAIARAVAPALKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDIS
CCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHEEE
VTGKLMDAIERRINAK
EHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQRRGYEIHYMEMDDLHLDQGVAIADTKVVELK
CEEEEEEECCCCCCEECCCCCEEEEEEHHHCCCEEEEEEECCEECCCCEEEECEEEEEEE
EDPNGWYEFKSEQTIALSDLDAVLMRKDPPFDTEYIYATYILERAEENGALIVNKPQSLR
CCCCCCEEECCCCEEEECCHHHHEECCCCCCCCCCEEEEEHHHHHCCCCEEEEECCHHHH
DCNEKLFTAWFPELTPTTIVTRKAEKIKAFREEHGDVILKPLDGMGGASIFRVKEGDPNV
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYAMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEEECC
TGEARPLSETDWAIARAVAPALKEKGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDIS
CCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHEEE
VTGKLMDAIERRINAK
EHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]