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The map label for this gene is cbiF [H]

Identifier: 218710614

GI number: 218710614

Start: 2855257

End: 2856069

Strand: Direct

Name: cbiF [H]

Synonym: VS_2668

Alternate gene names: 218710614

Gene position: 2855257-2856069 (Clockwise)

Preceding gene: 218710613

Following gene: 218710615

Centisome position: 86.54

GC content: 47.72

Gene sequence:

>813_bases
ATGACGGTATTTTTTATCGGTGCAGGGCCGGGTGACCCAGACTTAATCACAGTGAAAGGCGCACGCTTAGTCGAAAAGTG
CCCTGTGATTGTTTACGCAGGCAGTTTAGTGCCAAAAGCCGTTATCGATCGTGCTCGTCCTGACGCTTTGGTTTATAACT
CTGCTGACATGGATCTCGATGACCTAACGAATGTATACCTAACTGCACATAAAAAAGGTCAACACGTAGCACGAGTACAA
ACTGGCGATCTTTCAATTTATTCGTCACTTGCAGAACAGACACGTCGCTTGAACGAACTTGGCATTGATTGGAAAGTGGT
TCCGGGCGTGTCCTCTTTCCAAGCGAGTGCTGCGGCTCTTGGCCAAGAACTGACGCTACCTGAGAAGTGTCAGACCATTA
TTCTTAGCCGTGCATCAGGCCGAACGCCTGTACCTGAAAAAGAGAATCTACGCGCCCTTGCCTCTCACGAAGCAACACTG
TGTTTATTTCTTAGCGCAACATTAATTCGTAAAGTGGTTCGTGAATTATCAGATGTTTACCCGCAAGATTGGCCAGTGGC
TGTCGTTGAAAAAGCCAGTCACCCTGAACAGCGAATATTCCGTGCTACATTGGGTACGATCGCCCAAGTTATGCATGATG
AAGGGATTCGCTCCACCGCGATGATCATCGTCAGTAAAGTGCTAGGCGACAATGATTTTGTTGACTCTAAACTCTACGAT
CCGACCTTCTCACACGCTTGCCGAAAAGCACGTGTCGTTGAAGAGGGTGTAGCTGAAAGTGGAACATTGGAAGACGATAA
GAGCGCAGACTAA

Upstream 100 bases:

>100_bases
TATCAACTGAAAGACGGCAAGGTACTAGAAGGTCAAAAACGTGGCCATTCATTAAAAGTGGAACCTAAAACAGAATCAGT
GACGGAAGAGGCAAACTAAT

Downstream 100 bases:

>100_bases
CCTCGCTCATTAGCTGTTGATTGGTTTATGCGTTTATTCATTTTCACTATCCAGTCAGCAGCATTATTTGGCAGATATGA
TGATCTTTTTTGATTTAAAT

Product: precorrin-3 methylase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTVFFIGAGPGDPDLITVKGARLVEKCPVIVYAGSLVPKAVIDRARPDALVYNSADMDLDDLTNVYLTAHKKGQHVARVQ
TGDLSIYSSLAEQTRRLNELGIDWKVVPGVSSFQASAAALGQELTLPEKCQTIILSRASGRTPVPEKENLRALASHEATL
CLFLSATLIRKVVRELSDVYPQDWPVAVVEKASHPEQRIFRATLGTIAQVMHDEGIRSTAMIIVSKVLGDNDFVDSKLYD
PTFSHACRKARVVEEGVAESGTLEDDKSAD

Sequences:

>Translated_270_residues
MTVFFIGAGPGDPDLITVKGARLVEKCPVIVYAGSLVPKAVIDRARPDALVYNSADMDLDDLTNVYLTAHKKGQHVARVQ
TGDLSIYSSLAEQTRRLNELGIDWKVVPGVSSFQASAAALGQELTLPEKCQTIILSRASGRTPVPEKENLRALASHEATL
CLFLSATLIRKVVRELSDVYPQDWPVAVVEKASHPEQRIFRATLGTIAQVMHDEGIRSTAMIIVSKVLGDNDFVDSKLYD
PTFSHACRKARVVEEGVAESGTLEDDKSAD
>Mature_269_residues
TVFFIGAGPGDPDLITVKGARLVEKCPVIVYAGSLVPKAVIDRARPDALVYNSADMDLDDLTNVYLTAHKKGQHVARVQT
GDLSIYSSLAEQTRRLNELGIDWKVVPGVSSFQASAAALGQELTLPEKCQTIILSRASGRTPVPEKENLRALASHEATLC
LFLSATLIRKVVRELSDVYPQDWPVAVVEKASHPEQRIFRATLGTIAQVMHDEGIRSTAMIIVSKVLGDNDFVDSKLYDP
TFSHACRKARVVEEGVAESGTLEDDKSAD

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=243, Percent_Identity=29.2181069958848, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322922, Length=269, Percent_Identity=25.2788104089219, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 29372; Mature: 29241

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFFIGAGPGDPDLITVKGARLVEKCPVIVYAGSLVPKAVIDRARPDALVYNSADMDLD
CEEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCHHHHHHHHCCCCCEEEECCCCCCHH
DLTNVYLTAHKKGQHVARVQTGDLSIYSSLAEQTRRLNELGIDWKVVPGVSSFQASAAAL
HHHHEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
GQELTLPEKCQTIILSRASGRTPVPEKENLRALASHEATLCLFLSATLIRKVVRELSDVY
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
PQDWPVAVVEKASHPEQRIFRATLGTIAQVMHDEGIRSTAMIIVSKVLGDNDFVDSKLYD
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
PTFSHACRKARVVEEGVAESGTLEDDKSAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVFFIGAGPGDPDLITVKGARLVEKCPVIVYAGSLVPKAVIDRARPDALVYNSADMDLD
EEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCHHHHHHHHCCCCCEEEECCCCCCHH
DLTNVYLTAHKKGQHVARVQTGDLSIYSSLAEQTRRLNELGIDWKVVPGVSSFQASAAAL
HHHHEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
GQELTLPEKCQTIILSRASGRTPVPEKENLRALASHEATLCLFLSATLIRKVVRELSDVY
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
PQDWPVAVVEKASHPEQRIFRATLGTIAQVMHDEGIRSTAMIIVSKVLGDNDFVDSKLYD
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
PTFSHACRKARVVEEGVAESGTLEDDKSAD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]