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The map label for this gene is cbiH [H]

Identifier: 218710613

GI number: 218710613

Start: 2854416

End: 2855255

Strand: Direct

Name: cbiH [H]

Synonym: VS_2667

Alternate gene names: 218710613

Gene position: 2854416-2855255 (Clockwise)

Preceding gene: 218710612

Following gene: 218710614

Centisome position: 86.52

GC content: 46.67

Gene sequence:

>840_bases
ATGGCAAAACTAACACTGGTAGGCTTAGGGCCTGGCGCAATAGACCTAGTCACACCCGCAGCGCAACAAGCGGTAAAGAA
TGCAGATGTCGTTGCAGGCTACAAGCCGTATGTAGAATCAATCAGCACACTAATCGACACTCAAGAAGTCATTCAAAGTG
GTATGACTCGCGAATGGGACCGCGCTCAAGCCGCAGTAGAAGAAGCAGCAAAAGGTCGAGAAGTCGTATTGGTATGTTCT
GGGGATTCTGGCATGTACGCGATGGCGCCTTTAGTGCTTGAATTATTGGAGCAAAACGTAGAATTAAACGCACAAGTTGA
ACTGGATATTATTCCGGGCATCACCGCGGCTAACGCCTGTGCTTCTTTAGTGGGCGCTCCTCTTGGTCACGATAGTTGTA
CCATTTCTCTGTCTGACTTATTAACGCCTTGGGAAGTGATTACCAAGCGGATTGAAGCCGCGGCAATGGCTGATTTTGCG
ATTACCTTCTACAACCCTCGCTCTAAAAAGAGAACTCAGCACATTGAAACTGCACGTGAAATCCTTTTGGAACATCGTGA
CCCAAATACTCCCGTTGCTATCGTGAATGCTGCGTATCGTGATGAGCAAAAAGTCACACTGACAACACTGGCTGAATTTA
CCAAAGTGGAATTTAGTATGAACGCCGCGGTCGTGATAGGTAACAGCAACAGCTACCGATTTGGTGATTGGATCGTAACA
CCGCGTGGCTACACCAATAAATATCAACTGAAAGACGGCAAGGTACTAGAAGGTCAAAAACGTGGCCATTCATTAAAAGT
GGAACCTAAAACAGAATCAGTGACGGAAGAGGCAAACTAA

Upstream 100 bases:

>100_bases
AGTTCTGCATGCTCGATATGCCAGAACTGTTACAGATTATCGATAAGCAAATTAAAGAAGGCTTAATTCGTTTAGGTGCA
CCAGAGGAAGATAAATAATC

Downstream 100 bases:

>100_bases
TATGACGGTATTTTTTATCGGTGCAGGGCCGGGTGACCCAGACTTAATCACAGTGAAAGGCGCACGCTTAGTCGAAAAGT
GCCCTGTGATTGTTTACGCA

Product: precorrin-3B methylase CobJ

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAKLTLVGLGPGAIDLVTPAAQQAVKNADVVAGYKPYVESISTLIDTQEVIQSGMTREWDRAQAAVEEAAKGREVVLVCS
GDSGMYAMAPLVLELLEQNVELNAQVELDIIPGITAANACASLVGAPLGHDSCTISLSDLLTPWEVITKRIEAAAMADFA
ITFYNPRSKKRTQHIETAREILLEHRDPNTPVAIVNAAYRDEQKVTLTTLAEFTKVEFSMNAAVVIGNSNSYRFGDWIVT
PRGYTNKYQLKDGKVLEGQKRGHSLKVEPKTESVTEEAN

Sequences:

>Translated_279_residues
MAKLTLVGLGPGAIDLVTPAAQQAVKNADVVAGYKPYVESISTLIDTQEVIQSGMTREWDRAQAAVEEAAKGREVVLVCS
GDSGMYAMAPLVLELLEQNVELNAQVELDIIPGITAANACASLVGAPLGHDSCTISLSDLLTPWEVITKRIEAAAMADFA
ITFYNPRSKKRTQHIETAREILLEHRDPNTPVAIVNAAYRDEQKVTLTTLAEFTKVEFSMNAAVVIGNSNSYRFGDWIVT
PRGYTNKYQLKDGKVLEGQKRGHSLKVEPKTESVTEEAN
>Mature_278_residues
AKLTLVGLGPGAIDLVTPAAQQAVKNADVVAGYKPYVESISTLIDTQEVIQSGMTREWDRAQAAVEEAAKGREVVLVCSG
DSGMYAMAPLVLELLEQNVELNAQVELDIIPGITAANACASLVGAPLGHDSCTISLSDLLTPWEVITKRIEAAAMADFAI
TFYNPRSKKRTQHIETAREILLEHRDPNTPVAIVNAAYRDEQKVTLTTLAEFTKVEFSMNAAVVIGNSNSYRFGDWIVTP
RGYTNKYQLKDGKVLEGQKRGHSLKVEPKTESVTEEAN

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=249, Percent_Identity=27.710843373494, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 30319; Mature: 30188

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTLVGLGPGAIDLVTPAAQQAVKNADVVAGYKPYVESISTLIDTQEVIQSGMTREWD
CCEEEEEECCCCCHHHCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHH
RAQAAVEEAAKGREVVLVCSGDSGMYAMAPLVLELLEQNVELNAQVELDIIPGITAANAC
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
ASLVGAPLGHDSCTISLSDLLTPWEVITKRIEAAAMADFAITFYNPRSKKRTQHIETARE
HHHHCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHH
ILLEHRDPNTPVAIVNAAYRDEQKVTLTTLAEFTKVEFSMNAAVVIGNSNSYRFGDWIVT
HHHHCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHEEEECCEEEEEECCCCEEECCEEEC
PRGYTNKYQLKDGKVLEGQKRGHSLKVEPKTESVTEEAN
CCCCCCEEEECCCCEECCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKLTLVGLGPGAIDLVTPAAQQAVKNADVVAGYKPYVESISTLIDTQEVIQSGMTREWD
CEEEEEECCCCCHHHCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHH
RAQAAVEEAAKGREVVLVCSGDSGMYAMAPLVLELLEQNVELNAQVELDIIPGITAANAC
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
ASLVGAPLGHDSCTISLSDLLTPWEVITKRIEAAAMADFAITFYNPRSKKRTQHIETARE
HHHHCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHH
ILLEHRDPNTPVAIVNAAYRDEQKVTLTTLAEFTKVEFSMNAAVVIGNSNSYRFGDWIVT
HHHHCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHEEEECCEEEEEECCCCEEECCEEEC
PRGYTNKYQLKDGKVLEGQKRGHSLKVEPKTESVTEEAN
CCCCCCEEEECCCCEECCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]