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The map label for this gene is mscS [H]

Identifier: 218710593

GI number: 218710593

Start: 2829774

End: 2830649

Strand: Reverse

Name: mscS [H]

Synonym: VS_2644

Alternate gene names: 218710593

Gene position: 2830649-2829774 (Counterclockwise)

Preceding gene: 218710594

Following gene: 218710591

Centisome position: 85.8

GC content: 41.89

Gene sequence:

>876_bases
ATGCACATTATGGCTGATAGTTCGACAGCGCTTGAGACTCCAATTGTGGATGGTTTATCTCAGGCCGAGCAGTGGTTAAC
AAATAATTCAGATCTATTTATCCAATACGGTGTAAACATTATCTCTGCACTGATAATTCTATTTATTGGTAACTTGATTG
TTAAAGCAGTCGCGAATAGCGTATCTAAGGTTCTTGAGAAGAAGAAGATGGATCGAGCTGTTGTGGAGTTTATCCACGGT
TTAGTTCGTTATTTACTGTTTGTTATTGTTCTTATCGCTGCACTTGGTCGTTTAGGCGTTCAAACTGCATCTGTAGTTGC
TGTTATTGGTGCAGCTGGTTTAGCCGTTGGTCTTGCGCTGCAAGGCTCACTATCTAACTTTGCAGCGGGTGTACTTATCG
TTGCATTCCGTCCATTCAAGTCTGGTGACTACGTGGAAATTGGTGGTGTAGCGGGTTCAGTTGATTCAATTCAGATCTTC
CAAACTGTTTTAACAACACCAGACAACAAAATGGTTGTAGTGCCTAACGGAAGCGTTATCGGTAGCCCAATCACTAACTA
TTCTCGTCATGCGACGCGCCGTATTGACCTAATGATTGGTGTTTCTTACAACGCTGATCTTCAGAAGACAAAAGCGCTAC
TGACTAAGATCTGTGAATCTGATGAGCGCGTATTGAAAGATCCAGGTGTTCAAGTAGGTGTTCACACGCTAGCTGATTCT
TCAGTTAATTTTGTTGTTCGTCCATGGGTTAGTACTGCTGACTACTGGAATGTTTATTTCGACCTTATGCAAGCTATCAA
AGAAGGCTTGGATAACGAAGGTATCGAAATCCCATTCCCGCAAATGGATGTACACATGAATAAAGTTGAAGCTTAA

Upstream 100 bases:

>100_bases
TTTACTTTTAACACAGTGACTTAACAGGAGTTAAACTGTTTATTATTGTTAAAGTAAGTTTGGCTTAAGACAGTTTAGCT
CATAGCATATTATATAGAGG

Downstream 100 bases:

>100_bases
TTTCTAGTTAGTTTTTATAGCTATAAGCTTTTATAAGGAAAGGCGGATAACTTAGGTTATTCGCCTTTTTTGTTGGATGA
GTAAAGTTTGTTTGATGACT

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MHIMADSSTALETPIVDGLSQAEQWLTNNSDLFIQYGVNIISALIILFIGNLIVKAVANSVSKVLEKKKMDRAVVEFIHG
LVRYLLFVIVLIAALGRLGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIF
QTVLTTPDNKMVVVPNGSVIGSPITNYSRHATRRIDLMIGVSYNADLQKTKALLTKICESDERVLKDPGVQVGVHTLADS
SVNFVVRPWVSTADYWNVYFDLMQAIKEGLDNEGIEIPFPQMDVHMNKVEA

Sequences:

>Translated_291_residues
MHIMADSSTALETPIVDGLSQAEQWLTNNSDLFIQYGVNIISALIILFIGNLIVKAVANSVSKVLEKKKMDRAVVEFIHG
LVRYLLFVIVLIAALGRLGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIF
QTVLTTPDNKMVVVPNGSVIGSPITNYSRHATRRIDLMIGVSYNADLQKTKALLTKICESDERVLKDPGVQVGVHTLADS
SVNFVVRPWVSTADYWNVYFDLMQAIKEGLDNEGIEIPFPQMDVHMNKVEA
>Mature_291_residues
MHIMADSSTALETPIVDGLSQAEQWLTNNSDLFIQYGVNIISALIILFIGNLIVKAVANSVSKVLEKKKMDRAVVEFIHG
LVRYLLFVIVLIAALGRLGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIF
QTVLTTPDNKMVVVPNGSVIGSPITNYSRHATRRIDLMIGVSYNADLQKTKALLTKICESDERVLKDPGVQVGVHTLADS
SVNFVVRPWVSTADYWNVYFDLMQAIKEGLDNEGIEIPFPQMDVHMNKVEA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=276, Percent_Identity=50, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1786670, Length=226, Percent_Identity=27.8761061946903, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367355, Length=214, Percent_Identity=24.2990654205607, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787591, Length=291, Percent_Identity=25.7731958762887, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31402; Mature: 31402

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIMADSSTALETPIVDGLSQAEQWLTNNSDLFIQYGVNIISALIILFIGNLIVKAVANS
CEEECCCCCHHHCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VSKVLEKKKMDRAVVEFIHGLVRYLLFVIVLIAALGRLGVQTASVVAVIGAAGLAVGLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTVLTTPDNKMVVVPNGSVI
CCCHHHHHHHEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
GSPITNYSRHATRRIDLMIGVSYNADLQKTKALLTKICESDERVLKDPGVQVGVHTLADS
CCCCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHCCC
SVNFVVRPWVSTADYWNVYFDLMQAIKEGLDNEGIEIPFPQMDVHMNKVEA
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MHIMADSSTALETPIVDGLSQAEQWLTNNSDLFIQYGVNIISALIILFIGNLIVKAVANS
CEEECCCCCHHHCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VSKVLEKKKMDRAVVEFIHGLVRYLLFVIVLIAALGRLGVQTASVVAVIGAAGLAVGLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTVLTTPDNKMVVVPNGSVI
CCCHHHHHHHEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
GSPITNYSRHATRRIDLMIGVSYNADLQKTKALLTKICESDERVLKDPGVQVGVHTLADS
CCCCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHCCC
SVNFVVRPWVSTADYWNVYFDLMQAIKEGLDNEGIEIPFPQMDVHMNKVEA
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]