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The map label for this gene is iciA [H]

Identifier: 218710591

GI number: 218710591

Start: 2827991

End: 2828890

Strand: Reverse

Name: iciA [H]

Synonym: VS_2642

Alternate gene names: 218710591

Gene position: 2828890-2827991 (Counterclockwise)

Preceding gene: 218710593

Following gene: 218710590

Centisome position: 85.74

GC content: 47.0

Gene sequence:

>900_bases
TTGATGCGTGGATTGGATTATAAATGGATTGAAGCACTGGATGCCGTTGTGAAACAACGTAGCTTTGAGAGGGCCGCTGA
GCAGTTATACATCTCCCAATCAGCGGTGTCCCAACGTATCAAACAGCTAGAAAAGTGGTTAGCCCAGCCAGCGCTTGTGA
GAGAAAACCCACCAAGGCCTACACCAGCAGGAAAAAAGCTGTTGGGTTTGTATCGTCGTGTTCGCTTGTTAGAGCACGAG
CTTGTGCCGGAGTTAATGAATGAAGAGGGTACTCAACCACTATCTATATCCATAGCCACCAACGCTGATAGTTTGGCGAC
ATGGTTGTTACCTGCACTGTCGGATGTGATGAAGTCTCGTCAAGTCGAGTTGAACCTCGCGATTCATGGTGAGTCTAGAA
CCATTGAAAAAATAAAAAGCGGTGAGGTTGCCGGTGCGATCAGTTTAGAGCCCCAAGCGATTCCTGGATGCAGTGCTGAC
TACCTCGGTCGTATGGACTATGTGTGTGTGGCGAGTCCTGATTTTCATCAGCGTTACTTTTCAGAGGGCGTAAACTACGC
CACATTAAGCAAAGCGCCTGCGGTTTCTTATGATCAATACGATGATCTGCATAAGAAGTTTCTGCATGACCATTTTAACG
TACCGAGAGACAGTGTGATCAATCATACGGTCGGCAGTTCAGAAGCATTTGTGCGCTTGGCACTATCGGGTGTTGCCTAT
TGCTTGATTCCTCGATTGCAGATTATCGAGGAACTAGAGTCTGGTGCTCTGATTGATATTACCCCTGGCTTTCTGCTGTC
TTATCGCATCTATTGGCACCATTGGCAGCTTGAGAGTGGGGTGCTAAAAGAGGTATCCCAGGCGATATTAAGTTTTGCTC
ACAATCATTTACCTCAGTAA

Upstream 100 bases:

>100_bases
GGCGAAGATACTCCGACCCTTTAAATAAGTATAATTAATGATTTTAATCCATTATTAGAGTTGCTAATGTGGTATTGGGT
TAGCTTAAAAAGGAAGCAAA

Downstream 100 bases:

>100_bases
GTTGTTGGTTTTTTCAGCGTCAAAGTTCTGTTAGATGTGCACAATCAGGATTGGCTAAACGATAAATTACTCTATGATGG
AAGGACATTTGTTTAGCATA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MMRGLDYKWIEALDAVVKQRSFERAAEQLYISQSAVSQRIKQLEKWLAQPALVRENPPRPTPAGKKLLGLYRRVRLLEHE
LVPELMNEEGTQPLSISIATNADSLATWLLPALSDVMKSRQVELNLAIHGESRTIEKIKSGEVAGAISLEPQAIPGCSAD
YLGRMDYVCVASPDFHQRYFSEGVNYATLSKAPAVSYDQYDDLHKKFLHDHFNVPRDSVINHTVGSSEAFVRLALSGVAY
CLIPRLQIIEELESGALIDITPGFLLSYRIYWHHWQLESGVLKEVSQAILSFAHNHLPQ

Sequences:

>Translated_299_residues
MMRGLDYKWIEALDAVVKQRSFERAAEQLYISQSAVSQRIKQLEKWLAQPALVRENPPRPTPAGKKLLGLYRRVRLLEHE
LVPELMNEEGTQPLSISIATNADSLATWLLPALSDVMKSRQVELNLAIHGESRTIEKIKSGEVAGAISLEPQAIPGCSAD
YLGRMDYVCVASPDFHQRYFSEGVNYATLSKAPAVSYDQYDDLHKKFLHDHFNVPRDSVINHTVGSSEAFVRLALSGVAY
CLIPRLQIIEELESGALIDITPGFLLSYRIYWHHWQLESGVLKEVSQAILSFAHNHLPQ
>Mature_299_residues
MMRGLDYKWIEALDAVVKQRSFERAAEQLYISQSAVSQRIKQLEKWLAQPALVRENPPRPTPAGKKLLGLYRRVRLLEHE
LVPELMNEEGTQPLSISIATNADSLATWLLPALSDVMKSRQVELNLAIHGESRTIEKIKSGEVAGAISLEPQAIPGCSAD
YLGRMDYVCVASPDFHQRYFSEGVNYATLSKAPAVSYDQYDDLHKKFLHDHFNVPRDSVINHTVGSSEAFVRLALSGVAY
CLIPRLQIIEELESGALIDITPGFLLSYRIYWHHWQLESGVLKEVSQAILSFAHNHLPQ

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=298, Percent_Identity=50.3355704697987, Blast_Score=305, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33660; Mature: 33660

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRGLDYKWIEALDAVVKQRSFERAAEQLYISQSAVSQRIKQLEKWLAQPALVRENPPRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TPAGKKLLGLYRRVRLLEHELVPELMNEEGTQPLSISIATNADSLATWLLPALSDVMKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
QVELNLAIHGESRTIEKIKSGEVAGAISLEPQAIPGCSADYLGRMDYVCVASPDFHQRYF
CEEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCHHHHHHH
SEGVNYATLSKAPAVSYDQYDDLHKKFLHDHFNVPRDSVINHTVGSSEAFVRLALSGVAY
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
CLIPRLQIIEELESGALIDITPGFLLSYRIYWHHWQLESGVLKEVSQAILSFAHNHLPQ
HHHHHHHHHHHHHCCCEEEECCHHHHHEEEHEEEEECHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRGLDYKWIEALDAVVKQRSFERAAEQLYISQSAVSQRIKQLEKWLAQPALVRENPPRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TPAGKKLLGLYRRVRLLEHELVPELMNEEGTQPLSISIATNADSLATWLLPALSDVMKSR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
QVELNLAIHGESRTIEKIKSGEVAGAISLEPQAIPGCSADYLGRMDYVCVASPDFHQRYF
CEEEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCHHHHHHH
SEGVNYATLSKAPAVSYDQYDDLHKKFLHDHFNVPRDSVINHTVGSSEAFVRLALSGVAY
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
CLIPRLQIIEELESGALIDITPGFLLSYRIYWHHWQLESGVLKEVSQAILSFAHNHLPQ
HHHHHHHHHHHHHCCCEEEECCHHHHHEEEHEEEEECHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA