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The map label for this gene is ubiH [H]

Identifier: 218710584

GI number: 218710584

Start: 2821589

End: 2822767

Strand: Reverse

Name: ubiH [H]

Synonym: VS_2634

Alternate gene names: 218710584

Gene position: 2822767-2821589 (Counterclockwise)

Preceding gene: 218710585

Following gene: 218710583

Centisome position: 85.56

GC content: 47.24

Gene sequence:

>1179_bases
ATGGCTCAGTATGATGTTGTAATTGCTGGTGGCGCAATGGCGGGGGCGACCTTAGCCCTTGCTCTGAATCACCTCAGTCA
AGGTTCACTATCGATCGCGGTAGTAGAGCCTTATCAGGTTGATCATCAAGCTCACCCAGGCTTTGATTCTCGTTCGATCG
CTCTGTCTTATGGCACGGTGCAGATCCTCGATTCTTTGCGCCTTTGGCAATCTATCGCTCCGGTAGCGACCCCAATTAAA
GATATCCATGTGTCGGATAGGGGCCATGCCGGAATGACGGACATCTACAGTGAAGATCTTGCTGTTGATGCGCTCGGCTA
CGTGGTGGAGTTGGCGGATGTTGGTCGGATCTATCAGCAGAAATTGGAATCAGAAGCTACGATTACGATGCTTTGCCCTG
AGTCTGTTAGAACCGTTGAACGTGATGAATCGTTAACGACGATTGAGCTGACAAGTGGGCAAACAGTAACCACTAAGTTA
TTGGTTGCAGCTGACGGTGCAATCTCAACTTGTTGTCAGCAACTCAATATCTCATTGAGTGAGCATGACTTTGAACAAGT
CGCTGTGATTGCCAATATTGTTACTAGTGAGCCCCATCAAGGTCGAGCCTTTGAGCGTTTTACTCATCATGGGCCAGTTG
CTCTGTTACCGATGAGCGACAACCGTTTATCGCTAGTTTGGTGTTTGTCACCGGAACAAGCACAAAAAGTGGTGGCCTTA
AACGACAACGAATTTCTTGAGCAGTTACAGAATGACTTCGGTTGGCGACTTGGCCGACTAGAGAAAGTCGGCAAGCGTGC
GAGCTACCCACTGATTCTTCGCCACCGACAGCAAAATATCTCTCATCGATTTGCCATTGTTGGCAATGCTGCTCAAACAC
TTCACCCTATTGCGGGGCAAGGTTTTAATCTTGGCATTCGTGATGTGGCTTCCTTGGCGGAAGAATTATGTACTCAATTA
GATGACGTAGGTCGTTACACAGGTCTTGTTAATTTTAGAAAGCGCAGAGAACAAGACAGAGATACGACGATTACGTTGAC
TTCAAGCCTAGTTCATCTGTTCTCAAACGATTTTTTGACCGCTCGTATTGGGCGAAACCTTGGGTTAGCCGTAATTGATA
ACCTTCCACCACTTAAAGGTCCACTTTTGCGTCATACGCTTGGCCTAGTAGAAAGATAA

Upstream 100 bases:

>100_bases
AAAAGCGTGTGCACTAACGATTCATGCTGAATTCGGTGCTCGCCCATCTGAAGATGCGGCTCCAACCATTCATTAATCCG
CTTGTCGGTTTGAGGTTATA

Downstream 100 bases:

>100_bases
GGTAATAAATAATGATGCAAAGTGTTGATATCGCGATTGTTGGCGGTGGCATGGTTGGCCTAGCGCTTGCGGCTGCACTG
AAAGACAGCGATCTAAGAAT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MAQYDVVIAGGAMAGATLALALNHLSQGSLSIAVVEPYQVDHQAHPGFDSRSIALSYGTVQILDSLRLWQSIAPVATPIK
DIHVSDRGHAGMTDIYSEDLAVDALGYVVELADVGRIYQQKLESEATITMLCPESVRTVERDESLTTIELTSGQTVTTKL
LVAADGAISTCCQQLNISLSEHDFEQVAVIANIVTSEPHQGRAFERFTHHGPVALLPMSDNRLSLVWCLSPEQAQKVVAL
NDNEFLEQLQNDFGWRLGRLEKVGKRASYPLILRHRQQNISHRFAIVGNAAQTLHPIAGQGFNLGIRDVASLAEELCTQL
DDVGRYTGLVNFRKRREQDRDTTITLTSSLVHLFSNDFLTARIGRNLGLAVIDNLPPLKGPLLRHTLGLVER

Sequences:

>Translated_392_residues
MAQYDVVIAGGAMAGATLALALNHLSQGSLSIAVVEPYQVDHQAHPGFDSRSIALSYGTVQILDSLRLWQSIAPVATPIK
DIHVSDRGHAGMTDIYSEDLAVDALGYVVELADVGRIYQQKLESEATITMLCPESVRTVERDESLTTIELTSGQTVTTKL
LVAADGAISTCCQQLNISLSEHDFEQVAVIANIVTSEPHQGRAFERFTHHGPVALLPMSDNRLSLVWCLSPEQAQKVVAL
NDNEFLEQLQNDFGWRLGRLEKVGKRASYPLILRHRQQNISHRFAIVGNAAQTLHPIAGQGFNLGIRDVASLAEELCTQL
DDVGRYTGLVNFRKRREQDRDTTITLTSSLVHLFSNDFLTARIGRNLGLAVIDNLPPLKGPLLRHTLGLVER
>Mature_391_residues
AQYDVVIAGGAMAGATLALALNHLSQGSLSIAVVEPYQVDHQAHPGFDSRSIALSYGTVQILDSLRLWQSIAPVATPIKD
IHVSDRGHAGMTDIYSEDLAVDALGYVVELADVGRIYQQKLESEATITMLCPESVRTVERDESLTTIELTSGQTVTTKLL
VAADGAISTCCQQLNISLSEHDFEQVAVIANIVTSEPHQGRAFERFTHHGPVALLPMSDNRLSLVWCLSPEQAQKVVALN
DNEFLEQLQNDFGWRLGRLEKVGKRASYPLILRHRQQNISHRFAIVGNAAQTLHPIAGQGFNLGIRDVASLAEELCTQLD
DVGRYTGLVNFRKRREQDRDTTITLTSSLVHLFSNDFLTARIGRNLGLAVIDNLPPLKGPLLRHTLGLVER

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=427, Percent_Identity=27.1662763466042, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI32967307, Length=325, Percent_Identity=28.3076923076923, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=49.4897959183673, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1789273, Length=396, Percent_Identity=33.8383838383838, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1786883, Length=394, Percent_Identity=30.2030456852792, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=432, Percent_Identity=27.3148148148148, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321694, Length=456, Percent_Identity=24.5614035087719, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920760, Length=388, Percent_Identity=25.5154639175258, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43027; Mature: 42895

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYDVVIAGGAMAGATLALALNHLSQGSLSIAVVEPYQVDHQAHPGFDSRSIALSYGTV
CCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCCCCEEEEECCHH
QILDSLRLWQSIAPVATPIKDIHVSDRGHAGMTDIYSEDLAVDALGYVVELADVGRIYQQ
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLESEATITMLCPESVRTVERDESLTTIELTSGQTVTTKLLVAADGAISTCCQQLNISLS
HHCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCC
EHDFEQVAVIANIVTSEPHQGRAFERFTHHGPVALLPMSDNRLSLVWCLSPEQAQKVVAL
CCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEEEEEEECHHHCCEEEEE
NDNEFLEQLQNDFGWRLGRLEKVGKRASYPLILRHRQQNISHRFAIVGNAAQTLHPIAGQ
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEHHHHHCCCEEEEEECCHHHHHCCCCCC
GFNLGIRDVASLAEELCTQLDDVGRYTGLVNFRKRREQDRDTTITLTSSLVHLFSNDFLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCEE
ARIGRNLGLAVIDNLPPLKGPLLRHTLGLVER
EECCCCCCEEEECCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AQYDVVIAGGAMAGATLALALNHLSQGSLSIAVVEPYQVDHQAHPGFDSRSIALSYGTV
CCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCCCCEEEEECCHH
QILDSLRLWQSIAPVATPIKDIHVSDRGHAGMTDIYSEDLAVDALGYVVELADVGRIYQQ
HHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLESEATITMLCPESVRTVERDESLTTIELTSGQTVTTKLLVAADGAISTCCQQLNISLS
HHCCCCEEEEECCHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCC
EHDFEQVAVIANIVTSEPHQGRAFERFTHHGPVALLPMSDNRLSLVWCLSPEQAQKVVAL
CCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEECCCCEEEEEEECHHHCCEEEEE
NDNEFLEQLQNDFGWRLGRLEKVGKRASYPLILRHRQQNISHRFAIVGNAAQTLHPIAGQ
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEHHHHHCCCEEEEEECCHHHHHCCCCCC
GFNLGIRDVASLAEELCTQLDDVGRYTGLVNFRKRREQDRDTTITLTSSLVHLFSNDFLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCEE
ARIGRNLGLAVIDNLPPLKGPLLRHTLGLVER
EECCCCCCEEEECCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]