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The map label for this gene is pyrG [H]

Identifier: 218710559

GI number: 218710559

Start: 2796066

End: 2797706

Strand: Reverse

Name: pyrG [H]

Synonym: VS_2609

Alternate gene names: 218710559

Gene position: 2797706-2796066 (Counterclockwise)

Preceding gene: 218710560

Following gene: 218710558

Centisome position: 84.8

GC content: 46.01

Gene sequence:

>1641_bases
ATGACGACAAATTACATTTTTGTTACTGGCGGGGTTGTATCCTCTCTAGGTAAAGGTATTGCAGCAGCATCTCTTGCGGC
TATTTTAGAAGCTCGTGGTCTTAAAGTGACTATGATGAAGCTTGACCCTTACATCAACGTTGATCCAGGCACAATGAGCC
CGACTCAACACGGTGAAGTGTTCGTTACGGAAGATGGCGCTGAAACTGACCTTGACCTTGGTCACTACGAGCGATTCATT
CGCACCAAGATGACTAAGCGTAACAACTTCACTGCAGGTCGTGTTTACTCAGACGTACTCGCTAAAGAGCGTCGCGGTGA
TTACCTAGGTGCAACTATTCAGGTTATCCCGCACATCACTAACTCTATCAAAGAACGTGTAATTTCTGGCGCAGCTGGCC
ACGATATCGCGCTTGTTGAAGTTGGTGGTACGGTTGGTGATATCGAATCTCTACCATTCATGGAAGCGATTCGTCAGCTA
GCAGTAGAGCTAGGCCGTGAACGCGCAATGTTCATGCACCTTACTCTAGTGCCATACCTAGCAGCTGCGGGCGAAGTGAA
AACTAAGCCAACGCAACACTCTGTAAAAGAGCTGCTGTCTATTGGTATTCAGCCAGACATTCTAGTTTGTCGTTCAGACC
GCAACATTCCTTCGAACGAGCGTAAGAAAATTGCTCTGTTCTGTAATGTGCAAGAAAATGCAGTTATTTCTATGCGCGAT
GTTGACTCTATCTACAAGATCCCTCAGCTTATTAAAGCTCAAGGCACTGATGAACTTGTATGTAAGCGTTTCGGCATCAC
AGCTCCAGAAGCAGACCTGTCTGAATGGGAACAAGTAATTTACGAAGAAGCTAACCCAACGGGTGAAGTGACGATTGGTA
TGGTTGGTAAGTACATTGAACTACCAGACGCATACAAATCAGTAAATGAAGCGCTAAAACACGCGGGCTTGAAAAATCGC
CTAAGCGTTAATATTAAATACGTAGATTCACAAGACGTTGAGTCTCGTGGCGTAGAAGTTTTAGAAGGCCTAGATGCAAT
CCTAGTTCCTGGTGGCTTTGGTGATCGTGGTGTTGAAGGCAAGATTCTTGCTGCTCAATACGCTCGTGAAAACAAAGTAC
CTTACTTAGGTATCTGTCTAGGTATGCAAGTAGCACTTATCGAGTACGCTCGTAACGTTGCGAAAATGGAAGGGGCACAC
TCTTCTGAATTCTGTACTGAAACTAAGTACCCAGTTGTTGGTCTTATCACTGAGTGGACTGACGGCGAAGGTAAAGTTGA
AGAGCGTACAGAAACATCTGACCTTGGCGGCACAATGCGTCTTGGTTCACAGCTTTGTCACCTAGCGAAAGGGACAAAAG
CTTACGAATTATACGGTAGCGCGACGATCCATGAACGTCATCGTCACCGTTACGAAGTGAACAACAATCTTCGTCCACAA
ATTGAAAAAGCGGGCCTAAAAGTATCGGGTCTATCTGCGGACAAGAAACTGGTTGAAGTTATCGAGAACCCGAATCATCC
ATGGTTCGTTGCTGCTCAATTCCACCCAGAGTTCACTTCAACACCTCGCGATGGTCACCCATTGTTTGCAGGTTTCGTTA
AAGCAGCTGGCGAGTTCCAGCGCGGCGAATTAGAGAAGTAA

Upstream 100 bases:

>100_bases
TGTGATAGATTTCACGTTGTGGCGATAAGGGGCTTCTGGTATATTTTCATCCCGTCCAGAAGTAATCCATTTCCCTCATT
CAACCAATTTCAGGTTAAAC

Downstream 100 bases:

>100_bases
AAAGGAATACAGGTAGCTGCGTAAGTTGTGCTGCTACCTTTAAATTTGACATTTATATATTCAACGAGAAGGAAACATTC
AATGTCTAAGATCGTTAAAG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYSDVLAKERRGDYLGATIQVIPHITNSIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQL
AVELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRNIPSNERKKIALFCNVQENAVISMRD
VDSIYKIPQLIKAQGTDELVCKRFGITAPEADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVNIKYVDSQDVESRGVEVLEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAKMEGAH
SSEFCTETKYPVVGLITEWTDGEGKVEERTETSDLGGTMRLGSQLCHLAKGTKAYELYGSATIHERHRHRYEVNNNLRPQ
IEKAGLKVSGLSADKKLVEVIENPNHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGEFQRGELEK

Sequences:

>Translated_546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYSDVLAKERRGDYLGATIQVIPHITNSIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQL
AVELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRNIPSNERKKIALFCNVQENAVISMRD
VDSIYKIPQLIKAQGTDELVCKRFGITAPEADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVNIKYVDSQDVESRGVEVLEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAKMEGAH
SSEFCTETKYPVVGLITEWTDGEGKVEERTETSDLGGTMRLGSQLCHLAKGTKAYELYGSATIHERHRHRYEVNNNLRPQ
IEKAGLKVSGLSADKKLVEVIENPNHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGEFQRGELEK
>Mature_545_residues
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMTKRNNFTAGRVYSDVLAKERRGDYLGATIQVIPHITNSIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLA
VELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRNIPSNERKKIALFCNVQENAVISMRDV
DSIYKIPQLIKAQGTDELVCKRFGITAPEADLSEWEQVIYEEANPTGEVTIGMVGKYIELPDAYKSVNEALKHAGLKNRL
SVNIKYVDSQDVESRGVEVLEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAKMEGAHS
SEFCTETKYPVVGLITEWTDGEGKVEERTETSDLGGTMRLGSQLCHLAKGTKAYELYGSATIHERHRHRYEVNNNLRPQI
EKAGLKVSGLSADKKLVEVIENPNHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGEFQRGELEK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=556, Percent_Identity=43.3453237410072, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=43.705035971223, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=43.705035971223, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=43.705035971223, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=79.1127541589649, Blast_Score=885, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.752052545156, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.4035087719298, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322563, Length=565, Percent_Identity=40.5309734513274, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24664469, Length=556, Percent_Identity=42.2661870503597, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=40.6374501992032, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60002; Mature: 59871

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYSDVLAKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NSIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLAVELGRERAMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRNIPSNERKKIALFCNVQENAVISMRD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEHH
VDSIYKIPQLIKAQGTDELVCKRFGITAPEADLSEWEQVIYEEANPTGEVTIGMVGKYIE
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLKNRLSVNIKYVDSQDVESRGVEVLEGLDAILVPGGFGDRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENKVPYLGICLGMQVALIEYARNVAKMEGAHSSEFCTETKYPVVGLITEWT
CEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
DGEGKVEERTETSDLGGTMRLGSQLCHLAKGTKAYELYGSATIHERHRHRYEVNNNLRPQ
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCC
IEKAGLKVSGLSADKKLVEVIENPNHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGEFQ
HHHCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGELEK
CCCCCC
>Mature Secondary Structure 
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYSDVLAKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NSIKERVISGAAGHDIALVEVGGTVGDIESLPFMEAIRQLAVELGRERAMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRNIPSNERKKIALFCNVQENAVISMRD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEHH
VDSIYKIPQLIKAQGTDELVCKRFGITAPEADLSEWEQVIYEEANPTGEVTIGMVGKYIE
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLKNRLSVNIKYVDSQDVESRGVEVLEGLDAILVPGGFGDRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENKVPYLGICLGMQVALIEYARNVAKMEGAHSSEFCTETKYPVVGLITEWT
CEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
DGEGKVEERTETSDLGGTMRLGSQLCHLAKGTKAYELYGSATIHERHRHRYEVNNNLRPQ
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCC
IEKAGLKVSGLSADKKLVEVIENPNHPWFVAAQFHPEFTSTPRDGHPLFAGFVKAAGEFQ
HHHCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RGELEK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA