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The map label for this gene is eno [H]

Identifier: 218710558

GI number: 218710558

Start: 2794686

End: 2796038

Strand: Reverse

Name: eno [H]

Synonym: VS_2607

Alternate gene names: 218710558

Gene position: 2796038-2794686 (Counterclockwise)

Preceding gene: 218710559

Following gene: 218710557

Centisome position: 84.75

GC content: 45.16

Gene sequence:

>1353_bases
GTGCTGCTACCTTTAAATTTGACATTTATATATTCAACGAGAAGGAAACATTCAATGTCTAAGATCGTTAAAGTTCTAGG
TCGTGAAATCATCGATTCACGTGGTAACCCAACTGTAGAAGCTGAAGTACACCTAGAAGGCGGTTTCGTAGGTATGGCTG
CTGCTCCATCTGGCGCATCTACTGGTTCACGCGAAGCTCTTGAGCTACGTGACGGCGACAAATCACGTTTCCTAGGTAAA
GGTGTTCTTAAAGCTATTGAAGCTGTAAACGGCCCAATCGCTGAAGCTCTAGTAGGTTCTGACGCTAAAGCACAAGCTGA
CGTTGACCAAATCATGCTTGACCTAGACGGTACTGAAAACAAGTCTAAGTTCGGTGCGAACGCTATCCTAGCTGTATCTC
TAGCTAACGCAAAAGCTGCTGCTGCTGCGAAAGGCATGCCTCTTTACGAGCACATTGCAGAGCTAAACGGTACTGCTGGT
CAATTCTCTATGCCTCTACCAATGATGAACATCATCAACGGTGGTGAGCACGCAGATAACAACGTTGACATCCAAGAGTT
CATGATCCAACCAGTTGGCGCTAAGACTCTTAAAGAAGGTCTACGTATCGGCGCTGAAGTATTCCACAACCTAGCTAAAG
TTCTTAAGTCTAAAGGCTACAGCACTGCAGTTGGTGATGAAGGTGGTTTCGCTCCTAACCTTAAGTCTAACGCTGAAGCT
CTAGAAGTTATCGCAGAAGCTGTTGCAGCTGCTGGTTACGAACTAGGTAAAGACGTTACTCTTGCTATGGACTGTGCAGC
ATCTGAGTTCTTCGACAAAGAAGCTGGTATCTACAACATGAAGGGCGAAGGTAAAACTTTCACTTCTGTAGAGTTCAACC
ACTACCTAGCTGAGCTAGCGAACAACTTCCCAATCGTTTCTATCGAAGACGGTCTTGACGAGTCTGATTGGGATGGCTTC
AAGCACCAAACAGAACTTCTAGGCGACAAGCTTCAAATCGTTGGTGACGATCTGTTCGTTACAAACACGAAGATTCTTGC
TGAAGGTATCGAGAAAGGCGTAGCTAACTCTATCCTTATCAAGTTCAACCAAATCGGTTCTCTAACAGAGACTCTAGCTG
CTATCAAGATGGCTAAAGACGCTGGCTACACTGCAGTAATCTCTCACCGTTCTGGCGAAACTGAAGATGCAACTATCGCT
GATCTAGCGGTAGGTACAGCTGCTGGTCAAATCAAAACTGGTTCAATGAGCCGTTCTGACCGTGTTGCTAAGTACAACCA
ACTTATCCGTATCGAAGAAGCTCTAGGTTCTAAAGCTCCTTACAACGGTCTTAAAGAAGTTAAAGGTCAATAA

Upstream 100 bases:

>100_bases
CGATGGTCACCCATTGTTTGCAGGTTTCGTTAAAGCAGCTGGCGAGTTCCAGCGCGGCGAATTAGAGAAGTAAAAAGGAA
TACAGGTAGCTGCGTAAGTT

Downstream 100 bases:

>100_bases
TTCCTAGCGAATTATTAATCTAAGCTTTTTAAATGCCTCGCTTATGCGGGGCATTTTTTTGTCTGTAGAAAATGACGGGA
AGAAGCTCTAGGTTCTAAAA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLLPLNLTFIYSTRRKHSMSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGK
GVLKAIEAVNGPIAEALVGSDAKAQADVDQIMLDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTAG
QFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEA
LEVIAEAVAAAGYELGKDVTLAMDCAASEFFDKEAGIYNMKGEGKTFTSVEFNHYLAELANNFPIVSIEDGLDESDWDGF
KHQTELLGDKLQIVGDDLFVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIA
DLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGSKAPYNGLKEVKGQ

Sequences:

>Translated_450_residues
MLLPLNLTFIYSTRRKHSMSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGK
GVLKAIEAVNGPIAEALVGSDAKAQADVDQIMLDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTAG
QFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEA
LEVIAEAVAAAGYELGKDVTLAMDCAASEFFDKEAGIYNMKGEGKTFTSVEFNHYLAELANNFPIVSIEDGLDESDWDGF
KHQTELLGDKLQIVGDDLFVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIA
DLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGSKAPYNGLKEVKGQ
>Mature_450_residues
MLLPLNLTFIYSTRRKHSMSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGK
GVLKAIEAVNGPIAEALVGSDAKAQADVDQIMLDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTAG
QFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEA
LEVIAEAVAAAGYELGKDVTLAMDCAASEFFDKEAGIYNMKGEGKTFTSVEFNHYLAELANNFPIVSIEDGLDESDWDGF
KHQTELLGDKLQIVGDDLFVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIA
DLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGSKAPYNGLKEVKGQ

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=53.4722222222222, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=53.4722222222222, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.3888888888889, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=52.5462962962963, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=48.8372093023256, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1789141, Length=433, Percent_Identity=85.9122401847575, Blast_Score=735, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.6558891454965, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.6558891454965, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=47.3958333333333, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.6535796766744, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=46.5437788018433, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=46.5437788018433, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=46.5437788018433, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.8845265588915, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=49.8855835240275, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=49.8855835240275, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=49.8855835240275, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=49.8855835240275, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50.2325581395349, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50.2325581395349, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47669; Mature: 47669

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPLNLTFIYSTRRKHSMSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGAS
CEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCC
TGSREALELRDGDKSRFLGKGVLKAIEAVNGPIAEALVGSDAKAQADVDQIMLDLDGTEN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCHHHEEEECCCCCC
KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTAGQFSMPLPMMNIINGGEHADN
CHHCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCC
NVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHH
LEVIAEAVAAAGYELGKDVTLAMDCAASEFFDKEAGIYNMKGEGKTFTSVEFNHYLAELA
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHH
NNFPIVSIEDGLDESDWDGFKHQTELLGDKLQIVGDDLFVTNTKILAEGIEKGVANSILI
CCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEE
KFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSD
EEHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHH
RVAKYNQLIRIEEALGSKAPYNGLKEVKGQ
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
>Mature Secondary Structure
MLLPLNLTFIYSTRRKHSMSKIVKVLGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGAS
CEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCC
TGSREALELRDGDKSRFLGKGVLKAIEAVNGPIAEALVGSDAKAQADVDQIMLDLDGTEN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCHHHEEEECCCCCC
KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTAGQFSMPLPMMNIINGGEHADN
CHHCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCC
NVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHH
LEVIAEAVAAAGYELGKDVTLAMDCAASEFFDKEAGIYNMKGEGKTFTSVEFNHYLAELA
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHH
NNFPIVSIEDGLDESDWDGFKHQTELLGDKLQIVGDDLFVTNTKILAEGIEKGVANSILI
CCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEE
KFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSD
EEHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHH
RVAKYNQLIRIEEALGSKAPYNGLKEVKGQ
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA