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The map label for this gene is truD [H]

Identifier: 218710554

GI number: 218710554

Start: 2791688

End: 2792764

Strand: Reverse

Name: truD [H]

Synonym: VS_2603

Alternate gene names: 218710554

Gene position: 2792764-2791688 (Counterclockwise)

Preceding gene: 218710555

Following gene: 218710553

Centisome position: 84.65

GC content: 45.31

Gene sequence:

>1077_bases
GTGCGTAAGCACCAACACGAAAAGAACAGCATGTCAGATATTTTATCTTCATTGGCTTATCTAAACGGTAAACCAACTGC
GAAAGCAAAACTAAAAGCAAAAGCCGAACACTTTGTCGTTAATGAAGACTTAGGTTTTGAGTTCACTGGTGAAGGCGAAC
ACCTTATGGTTCGTATTCGTAAAACGGGTGAGAACACGAGCTTTGTAGCAAACGAGCTGGCTAAAGCGTGTGGTGTTAAG
TCGAAAGACGTGAGTTGGGCTGGTTTGAAAGACCGTCATGCTGTGACAGAACAGTGGTTGAGCGTGCATCTACCAAAAGG
TGAACCTGACTTTTCTGCTTTTCTTGCACAATACCCAAGCATTGAGATCTTGGCGACAGCACGCCACAACAAAAAGTTAC
GTCCAGGCGATTTAGTTGGCAACCAATTCGAGCTAACTTTGTCTGAAGTAACGGACTGCGATGATGTAGTCAAACGTTTG
GAAAAAGTCGCTCTAGTGGGCGTGCCAAATTACTTTGGTGCTCAGAGGTTTGGCAATGAAGGTAACAACTTATCTGAAGC
TCGTCGTTGGGGGCGTGAAAACGTTCGTACACGTAATCAAAATAAGCGCAGCTTGTACCTCTCTGCAGCTCGTTCTTGGA
TCTACAACTTAATTCTTTCTGACCGTATTGAGCACGATGCGTTTGCTTCGGCATTGGTCGGCGATATTGTAGTTAAAAAC
GATGCTCAACTTGCTGTGACAGCCGCTAATATCGAAGCTGTAAACCAAGATATCGCCAATGGTTCTGCGTTTATTACCGT
TGCTTTAGCTGGAGATAATGCGTTACCGACGACGAATGCGTCTCAAGCTCTAGAGCAGAAACACCTTGATGCTGAACCGG
ATCTAATGGCGCTTATTTGTGGCAACCGTATGCGCCATGATCGCCGTGAAGCCTCGTTGAAGCCGATGGGTTTAGTGTGG
AAAGTAAACGAAGACAATGTCACTCTTAAATTCTTTCTGGACGCAGGCTGCTTCGCAACCGCTATCGTTCGTGAGTTAGT
TGAAGAAGTACACGTAGAAAGAACCTACGAGCAATAA

Upstream 100 bases:

>100_bases
ATTAGCAATGTGAATGTAAAAGCGACGACCACTGAGCGTTTAGGTTTTACAGGTCGCAAAGAGGGCATCGCATGTGAAGC
GGTGGTTCTAATTACTAAAA

Downstream 100 bases:

>100_bases
TGATTCTGCTTGGGTCTAAGTCACTTAATCTTGGAATTTAAGTGACTTGGCATTGGTACAAAAGAAAGGATACAAGATGA
AGATTTTACTCAGCAACGAT

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRKHQHEKNSMSDILSSLAYLNGKPTAKAKLKAKAEHFVVNEDLGFEFTGEGEHLMVRIRKTGENTSFVANELAKACGVK
SKDVSWAGLKDRHAVTEQWLSVHLPKGEPDFSAFLAQYPSIEILATARHNKKLRPGDLVGNQFELTLSEVTDCDDVVKRL
EKVALVGVPNYFGAQRFGNEGNNLSEARRWGRENVRTRNQNKRSLYLSAARSWIYNLILSDRIEHDAFASALVGDIVVKN
DAQLAVTAANIEAVNQDIANGSAFITVALAGDNALPTTNASQALEQKHLDAEPDLMALICGNRMRHDRREASLKPMGLVW
KVNEDNVTLKFFLDAGCFATAIVRELVEEVHVERTYEQ

Sequences:

>Translated_358_residues
MRKHQHEKNSMSDILSSLAYLNGKPTAKAKLKAKAEHFVVNEDLGFEFTGEGEHLMVRIRKTGENTSFVANELAKACGVK
SKDVSWAGLKDRHAVTEQWLSVHLPKGEPDFSAFLAQYPSIEILATARHNKKLRPGDLVGNQFELTLSEVTDCDDVVKRL
EKVALVGVPNYFGAQRFGNEGNNLSEARRWGRENVRTRNQNKRSLYLSAARSWIYNLILSDRIEHDAFASALVGDIVVKN
DAQLAVTAANIEAVNQDIANGSAFITVALAGDNALPTTNASQALEQKHLDAEPDLMALICGNRMRHDRREASLKPMGLVW
KVNEDNVTLKFFLDAGCFATAIVRELVEEVHVERTYEQ
>Mature_358_residues
MRKHQHEKNSMSDILSSLAYLNGKPTAKAKLKAKAEHFVVNEDLGFEFTGEGEHLMVRIRKTGENTSFVANELAKACGVK
SKDVSWAGLKDRHAVTEQWLSVHLPKGEPDFSAFLAQYPSIEILATARHNKKLRPGDLVGNQFELTLSEVTDCDDVVKRL
EKVALVGVPNYFGAQRFGNEGNNLSEARRWGRENVRTRNQNKRSLYLSAARSWIYNLILSDRIEHDAFASALVGDIVVKN
DAQLAVTAANIEAVNQDIANGSAFITVALAGDNALPTTNASQALEQKHLDAEPDLMALICGNRMRHDRREASLKPMGLVW
KVNEDNVTLKFFLDAGCFATAIVRELVEEVHVERTYEQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Escherichia coli, GI1789102, Length=337, Percent_Identity=44.213649851632, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39841; Mature: 39841

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHQHEKNSMSDILSSLAYLNGKPTAKAKLKAKAEHFVVNEDLGFEFTGEGEHLMVRIR
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHEEEEECCCCEEECCCCCEEEEEEE
KTGENTSFVANELAKACGVKSKDVSWAGLKDRHAVTEQWLSVHLPKGEPDFSAFLAQYPS
ECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHCCC
IEILATARHNKKLRPGDLVGNQFELTLSEVTDCDDVVKRLEKVALVGVPNYFGAQRFGNE
EEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHEEEECCCHHHHHHCCCC
GNNLSEARRWGRENVRTRNQNKRSLYLSAARSWIYNLILSDRIEHDAFASALVGDIVVKN
CCCHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DAQLAVTAANIEAVNQDIANGSAFITVALAGDNALPTTNASQALEQKHLDAEPDLMALIC
CCEEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEC
GNRMRHDRREASLKPMGLVWKVNEDNVTLKFFLDAGCFATAIVRELVEEVHVERTYEQ
CCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKHQHEKNSMSDILSSLAYLNGKPTAKAKLKAKAEHFVVNEDLGFEFTGEGEHLMVRIR
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHEEEEECCCCEEECCCCCEEEEEEE
KTGENTSFVANELAKACGVKSKDVSWAGLKDRHAVTEQWLSVHLPKGEPDFSAFLAQYPS
ECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHCCC
IEILATARHNKKLRPGDLVGNQFELTLSEVTDCDDVVKRLEKVALVGVPNYFGAQRFGNE
EEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHEEEECCCHHHHHHCCCC
GNNLSEARRWGRENVRTRNQNKRSLYLSAARSWIYNLILSDRIEHDAFASALVGDIVVKN
CCCHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DAQLAVTAANIEAVNQDIANGSAFITVALAGDNALPTTNASQALEQKHLDAEPDLMALIC
CCEEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEC
GNRMRHDRREASLKPMGLVWKVNEDNVTLKFFLDAGCFATAIVRELVEEVHVERTYEQ
CCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA