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The map label for this gene is trmD [H]

Identifier: 218710517

GI number: 218710517

Start: 2746227

End: 2746967

Strand: Reverse

Name: trmD [H]

Synonym: VS_2556

Alternate gene names: 218710517

Gene position: 2746967-2746227 (Counterclockwise)

Preceding gene: 218710518

Following gene: 218710516

Centisome position: 83.26

GC content: 45.21

Gene sequence:

>741_bases
ATGTGGGTTGGCATTATTAGCCTTTTTCCTGAAATGTTCCGTTCTGTTACTGATTTTGGAGTAACAGGTCAAGCGGTTAA
AAAAGGTCTTTTATCTATTGAAACATGGAATCCTCGTGATTTCACTCATGATAAACATCGCACTGTTGATGACAGACCTT
ACGGTGGTGGTCCTGGCATGTTAATGATGGTTCAGCCTTTACGCGATGCTATCCAAACAGCCAAACAGGCAGCACCGGGA
AAGACGAAGGTTATTTACCTTTCACCTCAAGGTCGTAAACTCGACCAACAAGGTGTTGAAGAGCTGGCAACAAACGAGAA
CTTGCTTCTTATTTGTGGTCGCTATGAAGGGGTAGATGAGCGCATCATTCAATCTGAAGTCGACGAAGAATGGTCGATTG
GAGATTTTGTGATGACGGGTGGCGAGCTGCCAGCCATGACGTTGATTGATTCAGTATCTCGGTTTGTTCCGGGTGTACTT
GGAGATTTCGCATCAGCAGAAGAAGACTCTTTTGCAAATGGTTTGCTAGATTGTCCCCATTATACGCGCCCTGAGGTGCT
AGACGATAAAGATGTGCCATCGGTACTCAAGTCTGGAAACCATAAGGACATTCGTCGCTGGCGATTGAAACAATCGTTGG
GCCGAACTTGGCTAAGAAGACCAGAACTCCTGGAAAACCTAGCTCTGACTGACGAACAGGAACAATTACTGGCTGAATTC
ATTAAAGAGCAACGCTCTTAA

Upstream 100 bases:

>100_bases
TTGAAGAGCAAGTGATCAAGAAAGTTGATCGCGAAGCTCGCCGGATCGAAGTTGACTGGGATCCTGGATTCTAACTCCAA
ATTACAGAGCGAGAGAACAC

Downstream 100 bases:

>100_bases
CGAAAAGCAAGCAGTAACCTATTAAATTTAGTATCAGTTTATTCTAGGAATTTATACAAATGAGCAACATCATCAAGGCT
CTTGAAGAAGAGCAACTAAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MWVGIISLFPEMFRSVTDFGVTGQAVKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAIQTAKQAAPG
KTKVIYLSPQGRKLDQQGVEELATNENLLLICGRYEGVDERIIQSEVDEEWSIGDFVMTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDDKDVPSVLKSGNHKDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLAEF
IKEQRS

Sequences:

>Translated_246_residues
MWVGIISLFPEMFRSVTDFGVTGQAVKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAIQTAKQAAPG
KTKVIYLSPQGRKLDQQGVEELATNENLLLICGRYEGVDERIIQSEVDEEWSIGDFVMTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDDKDVPSVLKSGNHKDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLAEF
IKEQRS
>Mature_246_residues
MWVGIISLFPEMFRSVTDFGVTGQAVKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGMLMMVQPLRDAIQTAKQAAPG
KTKVIYLSPQGRKLDQQGVEELATNENLLLICGRYEGVDERIIQSEVDEEWSIGDFVMTGGELPAMTLIDSVSRFVPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDDKDVPSVLKSGNHKDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLAEF
IKEQRS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=79.9180327868852, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27730; Mature: 27730

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVGIISLFPEMFRSVTDFGVTGQAVKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCH
LMMVQPLRDAIQTAKQAAPGKTKVIYLSPQGRKLDQQGVEELATNENLLLICGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVMTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFANGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YTRPEVLDDKDVPSVLKSGNHKDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLAEF
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
IKEQRS
HHHHCC
>Mature Secondary Structure
MWVGIISLFPEMFRSVTDFGVTGQAVKKGLLSIETWNPRDFTHDKHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCH
LMMVQPLRDAIQTAKQAAPGKTKVIYLSPQGRKLDQQGVEELATNENLLLICGRYEGVDE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIQSEVDEEWSIGDFVMTGGELPAMTLIDSVSRFVPGVLGDFASAEEDSFANGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YTRPEVLDDKDVPSVLKSGNHKDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLAEF
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
IKEQRS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA