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The map label for this gene is pilB [H]

Identifier: 218710509

GI number: 218710509

Start: 2739955

End: 2741649

Strand: Direct

Name: pilB [H]

Synonym: VS_2548

Alternate gene names: 218710509

Gene position: 2739955-2741649 (Clockwise)

Preceding gene: 218710508

Following gene: 218710510

Centisome position: 83.05

GC content: 47.61

Gene sequence:

>1695_bases
CTGTGTGTAGTGCTAACCAACCTCCCAACAGTTCTCCGTCAGGCTAACTTACTTAGCCTGACTCAAGAACAAGCCGTTGC
GGAACATGTACAAGCTTCAGGTATTTCTACACCTGAAGCTTTGCTCACTCTGGGCTTATTCACCAGCGAGAGCCTCGCTA
ATAACATCAAAACCATTTTCGGTTTGCCGTTAGTGCAACTGGCCAATACTGACTATGAAATCTTGTGTGACCAATTGGGA
CTTCGAGAATTGATCACTAAGTATCGTGCTATCCCAGTTGTCCTCACTAACTCTACTCTCACGCTCGCCTCTGCCGATCC
AACCGACTTGCAAGCTGAAGACGATTTTAGATTCGCGACTGGATTGCAGATAGAACTCGTTGTCGCTAACTATTCAGAAT
TAGAAGGCGCCATACGAAAGCTGTATGGTCGCTCTATTTCCGGGCAAGACTCTAAGCGCAAAGAGATCACCCAAGATGAG
CTCGCCAATCTTGTTGAAGTCTCTGACGATGAAATGAGTTCAATTGAAGATCTCAGTCAAGACGATTCCCCTGTCAGTCG
ATTTATCAATCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCAGACATCCACTTCGAACCTTATGAAGAAAACTACC
GAGTGCGTCTGCGTTGCGATGGCATTCTTGTTGAGGTCCAACAGCCCGCGTCTCATTTAAGCCGTCGGTTATCCGCTCGT
TTAAAGATTCTCGCCAAACTTGATATCGCTGAACGTCGCTTACCTCAAGACGGCCGTATTAAGCTGCGTTTGAACGACGA
GCTGGCGATCGATATGCGTGTGTCGACTCTACCGACATTGTGGGGAGAAAAGATCGTACTGCGTCTTCTAGACAGCAGCG
CAGCCAATTTAGATATCGATAAATTAGGTTACAGCGAGGATCAAAAAGCGCTCTATCTCAATGCCCTAAAACGCCCGCAA
GGGATGATCTTAATGACTGGGCCCACAGGCAGTGGCAAAACGGTCTCTCTTTACACTGGACTTCGCATGCTTAACACCTC
TGAGCGTAATATCTCAACAGCTGAAGATCCGGTGGAGATCAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCAAAGA
TCGGTTTTGGGTTTGCCGAAGCACTACGATCGTTCTTGCGACAAGACCCTGATGTAGTGATGGTCGGTGAGATCCGTGAT
TTAGAAACCGCAGAGATCGCGATCAAAGCGTCGCAAACGGGTCACTTAGTCCTCTCGACACTCCATACTAACTCAGCTTC
GGAAACCGTGACTCGGCTCGCCCATATGGGGATTGAGCCCTTTAACCTCGCTTCGTCATTGAGCCTGATTATCGCGCAGC
GACTGGCAAGGCGACTGTGCAACCACTGTAAAGCAGCTGACAACTCGCCGGACATATTTTTACGTCACTCTATTCCTAAT
AGCCAAAACATCTACCAAGCCAATCCTCAAGGATGCAATGAGTGTAATCAGGGTTACTCCGGTCGAGTGGGTATCTACGA
AGTGATGCCGTTTACCGACAAACTAAAAAATAGTCTGATTAATAAACCTAACGCGTTAGAGATTGAAGATCTCGCGCGTA
GAGAAGGAATGAGAACACTGCAAGAATCCGGTATCGATAAGTTGCTTGAAGGCACCACCAGCTATCAAGAACTGCAACGT
GTTCTGTATCTATAA

Upstream 100 bases:

>100_bases
GAGCAACTAGCTCAATAAGTGCAGCAACCTTAACATTTGCTCGAGGTAACTCTGGGTGGGTTTGTACTAACAGTAAACAA
GCAACGATCCCTCTAGATGG

Downstream 100 bases:

>100_bases
TCCATCGGAGCACTCATGAGAACTAAACTGCAGTCACAATTAAAAAACTACCACTGGAAAGGCATAAATAGCTCAGGCAA
AAAGGTGTCCGGTCAAACCT

Product: type IV pilin assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MCVVLTNLPTVLRQANLLSLTQEQAVAEHVQASGISTPEALLTLGLFTSESLANNIKTIFGLPLVQLANTDYEILCDQLG
LRELITKYRAIPVVLTNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDE
LANLVEVSDDEMSSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSAR
LKILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQ
GMILMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRD
LETAEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPN
SQNIYQANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTLQESGIDKLLEGTTSYQELQR
VLYL

Sequences:

>Translated_564_residues
MCVVLTNLPTVLRQANLLSLTQEQAVAEHVQASGISTPEALLTLGLFTSESLANNIKTIFGLPLVQLANTDYEILCDQLG
LRELITKYRAIPVVLTNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDE
LANLVEVSDDEMSSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSAR
LKILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQ
GMILMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRD
LETAEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPN
SQNIYQANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTLQESGIDKLLEGTTSYQELQR
VLYL
>Mature_564_residues
MCVVLTNLPTVLRQANLLSLTQEQAVAEHVQASGISTPEALLTLGLFTSESLANNIKTIFGLPLVQLANTDYEILCDQLG
LRELITKYRAIPVVLTNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDE
LANLVEVSDDEMSSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSAR
LKILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQ
GMILMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRD
LETAEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPN
SQNIYQANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTLQESGIDKLLEGTTSYQELQR
VLYL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=48.2926829268293, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786296, Length=482, Percent_Identity=41.0788381742739, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI87082188, Length=283, Percent_Identity=31.4487632508834, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62535; Mature: 62535

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVVLTNLPTVLRQANLLSLTQEQAVAEHVQASGISTPEALLTLGLFTSESLANNIKTIF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLPLVQLANTDYEILCDQLGLRELITKYRAIPVVLTNSTLTLASADPTDLQAEDDFRFAT
CCCHHHHCCCCHHHHHHHCCHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCCCCEEEC
GLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELANLVEVSDDEMSSIEDLSQ
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHCCC
DDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSAR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHH
LKILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDID
HHHHHHCCHHHHHCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHHCCCCCCCCHH
KLGYSEDQKALYLNALKRPQGMILMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEI
HCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHCCCCCCCCCCCCCCEEE
NLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLETAEIAIKASQTGHLVLST
EEECCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEE
LHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPN
ECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
SQNIYQANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTL
CCCEEECCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QESGIDKLLEGTTSYQELQRVLYL
HHHCHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MCVVLTNLPTVLRQANLLSLTQEQAVAEHVQASGISTPEALLTLGLFTSESLANNIKTIF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLPLVQLANTDYEILCDQLGLRELITKYRAIPVVLTNSTLTLASADPTDLQAEDDFRFAT
CCCHHHHCCCCHHHHHHHCCHHHHHHHHCCEEEEEECCEEEEECCCCCCCCCCCCCEEEC
GLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELANLVEVSDDEMSSIEDLSQ
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHCCC
DDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSAR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHH
LKILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDID
HHHHHHCCHHHHHCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHHCCCCCCCCHH
KLGYSEDQKALYLNALKRPQGMILMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEI
HCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHCCCCCCCCCCCCCCEEE
NLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLETAEIAIKASQTGHLVLST
EEECCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEE
LHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADNSPDIFLRHSIPN
ECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
SQNIYQANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTL
CCCEEECCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QESGIDKLLEGTTSYQELQRVLYL
HHHCHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]