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The map label for this gene is ampD [H]

Identifier: 218710506

GI number: 218710506

Start: 2737725

End: 2738273

Strand: Reverse

Name: ampD [H]

Synonym: VS_2545

Alternate gene names: 218710506

Gene position: 2738273-2737725 (Counterclockwise)

Preceding gene: 218710516

Following gene: 218710504

Centisome position: 83.0

GC content: 46.99

Gene sequence:

>549_bases
ATGACGATGAGCTTCAACGGATGGTACGACAATGCTCGGCATGTTCCTTCTCCGTATTTTGATGCTAGACCCAGCACCGA
CGATATCTCTCTGCTGGTGGTCCATAACATTAGTTTACCACCTGGCCAATTTGGCGGCCCTTACATTGAACAGTTTTTCA
CGGGCAAACTTGATCCGGCAGATCATCCATTTTTTAAAGTGATTCATCAAATGGGCGTTTCGGCACATTGCTTGATTCGC
CGTGATGGTGAGGTGGTACAGTTTGTGTCATTTCTTGACCGAGCTTGGCACGCAGGCCAGTCGAGCTTTGCGGGGCGTAA
AAGGTGCAATGATTACTCGATTGGTATTGAGCTGGAAGGAAGCGACTTTATCGCTTATACAGAGCTGCAGTACCAAGCGC
TTACTCGATTAACAGAAACGATCGTATCGACTTTCCCTCAAATAACCACCGAACGTATCACTGGTCATCAATATATAGCC
CCGTTGCGCAAAACTGACCCTGGTTTGGTTTTCGACTGGCAGAAGTTTAAAGGCGAGCTTAAGGTTTAA

Upstream 100 bases:

>100_bases
TGATACTCTTGCTTAACTTCAAATTAAAGAGGCTAGCGCGACTGCTCTATGGGCAAAGTCACAATCTTATTGCGCTAGTA
GAAAAGAAAGCGAGACTAAG

Downstream 100 bases:

>100_bases
GCCTCATTATCGAAGGTGCTCTTATTGTTGCTGGTGGTTGAATAGTCAACGCCTAGCATAGTGACTTGTGAATTTTTGTT
GTTGCGATGTAAATAAAGTG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTMSFNGWYDNARHVPSPYFDARPSTDDISLLVVHNISLPPGQFGGPYIEQFFTGKLDPADHPFFKVIHQMGVSAHCLIR
RDGEVVQFVSFLDRAWHAGQSSFAGRKRCNDYSIGIELEGSDFIAYTELQYQALTRLTETIVSTFPQITTERITGHQYIA
PLRKTDPGLVFDWQKFKGELKV

Sequences:

>Translated_182_residues
MTMSFNGWYDNARHVPSPYFDARPSTDDISLLVVHNISLPPGQFGGPYIEQFFTGKLDPADHPFFKVIHQMGVSAHCLIR
RDGEVVQFVSFLDRAWHAGQSSFAGRKRCNDYSIGIELEGSDFIAYTELQYQALTRLTETIVSTFPQITTERITGHQYIA
PLRKTDPGLVFDWQKFKGELKV
>Mature_181_residues
TMSFNGWYDNARHVPSPYFDARPSTDDISLLVVHNISLPPGQFGGPYIEQFFTGKLDPADHPFFKVIHQMGVSAHCLIRR
DGEVVQFVSFLDRAWHAGQSSFAGRKRCNDYSIGIELEGSDFIAYTELQYQALTRLTETIVSTFPQITTERITGHQYIAP
LRKTDPGLVFDWQKFKGELKV

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=59.3023255813954, Blast_Score=223, Evalue=6e-60,
Organism=Escherichia coli, GI1787092, Length=120, Percent_Identity=38.3333333333333, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20706; Mature: 20574

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSFNGWYDNARHVPSPYFDARPSTDDISLLVVHNISLPPGQFGGPYIEQFFTGKLDPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCC
DHPFFKVIHQMGVSAHCLIRRDGEVVQFVSFLDRAWHAGQSSFAGRKRCNDYSIGIELEG
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEECC
SDFIAYTELQYQALTRLTETIVSTFPQITTERITGHQYIAPLRKTDPGLVFDWQKFKGEL
CCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCCCCCCCCEEEEHHHCCCCC
KV
CC
>Mature Secondary Structure 
TMSFNGWYDNARHVPSPYFDARPSTDDISLLVVHNISLPPGQFGGPYIEQFFTGKLDPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCC
DHPFFKVIHQMGVSAHCLIRRDGEVVQFVSFLDRAWHAGQSSFAGRKRCNDYSIGIELEG
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEECC
SDFIAYTELQYQALTRLTETIVSTFPQITTERITGHQYIAPLRKTDPGLVFDWQKFKGEL
CCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCCCCCCCCEEEEHHHCCCCC
KV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]