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The map label for this gene is hemL [H]

Identifier: 218710463

GI number: 218710463

Start: 2677726

End: 2679021

Strand: Direct

Name: hemL [H]

Synonym: VS_2502

Alternate gene names: 218710463

Gene position: 2677726-2679021 (Clockwise)

Preceding gene: 218710459

Following gene: 218710464

Centisome position: 81.16

GC content: 46.99

Gene sequence:

>1296_bases
ATGACCAAATCAGCAGAGCTGTACGAAAAAGCACAGCAAACTATTCCTGGTGGCGTAAACTCTCCAGTTCGTGCATTCAA
TGGCGTAGGTGGTTCTCCTATCTTCGTTGAACGCGCTGATGGCCCACTTATTTTTGATGCTGATGGTAAAGCATATATCG
ATTACGTTGGCTCTTGGGGTCCAATGATCCTTGGTCACAACCACGTTGTTATTCGTGACGCTGTTATTGCAGCAGCTCAA
CGCGGCCTTAGCTTTGGTGCTCCAACCGAAACTGAAATCAAGATGGCTGAACTTGTATCTGAGATGGTTCCATCAATGGA
ACAGCTTCGTATGGTGAGCTCAGGTACAGAAGCAACAATGAGTGCGATTCGTCTCGCTCGTGGCTTTACTGGTCGTGACA
AAATCCTTAAGTTTGAAGGTTGTTACCACGGTCACGCAGACAGCCTACTTGTAAAAGCAGGTTCTGGCGCACTGACTTTA
GGTCAACCAAGCTCTCCAGGCGTACCGGCTGATTTTGCGAAACTAACGCTAACTGCAACTTTCAATAATCTAGATTCAGT
ACGTGAAATCTTCGCAGCAAACAAAGGTGAGATCGCGTGTATCATCGTTGAGCCTGTCGCGGGCAACATGAACTGCATCC
CACCAGTAGAAGGCTTCCACGAAGGTCTACGTGAGATCTGTGATCAAGAAGGTGCATTGCTAATCTTTGATGAAGTAATG
ACAGGTTTCCGCGTTGCTGAAGGTTGTGCTCAGGCTTACTACAACATCAAGCCAGACTTAACGTGTCTTGGTAAAGTGAT
TGGCGGCGGCATGCCAGTGGGTGCTTTTGGTGGTCGTAAAGACGTGATGCAATACATCGCACCAACGGGGCCTGTTTACC
AAGCAGGTACGCTTTCAGGTAACCCTGTTGCAATGGCTGCTGGCTACGCATGTTTGAATCTTCTAAGAGAAGAAGGCAAC
GAAAAGCGTCTAGCGTCTAAAACTAAGCAGTTGGCAAATGGCTTCAAGCAACTTGCTGACAAGCACGGCATCCCTATGCT
AGTTCACCAAGTTGGCGGTATGTTTGGTTTCTTCTTCACAGACCAAGAAACAGTGACCTGCTACGAAGATGTAACTAAGT
GTGATGTAGAACGCTTCAAGCGCTTCTTCCATCTAATGTTAGATCACGGTGTTTACCTTGCACCTTCAGCATTCGAAGCA
AGCTTTACTTCTCTTGCTCATGGTTCAAAAGAACTGGATGCAACACTAGAAGCTGCTGACCGCTCTCTTGCGATCATTGC
TGCTGAAAGCAAATAA

Upstream 100 bases:

>100_bases
ACCAGATTCGGGTGATTATATCAGCGAAGGCATTCCTAGTTGGATTCCGGATAGGTACAATGCGTGCCAATTGGTCCGAC
AGTCAAAAGAGGATATATCA

Downstream 100 bases:

>100_bases
TCGAAAGCACTTTATAAGTCGCTAACCGATTGAATTTAGGGCTGCATTAGATGCAGCCCTTTTTAATACATCACGCTCGT
TAACCACAAATATGACAGAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTKSAELYEKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHVVIRDAVIAAAQ
RGLSFGAPTETEIKMAELVSEMVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKLTLTATFNNLDSVREIFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVAEGCAQAYYNIKPDLTCLGKVIGGGMPVGAFGGRKDVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLNLLREEGN
EKRLASKTKQLANGFKQLADKHGIPMLVHQVGGMFGFFFTDQETVTCYEDVTKCDVERFKRFFHLMLDHGVYLAPSAFEA
SFTSLAHGSKELDATLEAADRSLAIIAAESK

Sequences:

>Translated_431_residues
MTKSAELYEKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHVVIRDAVIAAAQ
RGLSFGAPTETEIKMAELVSEMVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKLTLTATFNNLDSVREIFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVAEGCAQAYYNIKPDLTCLGKVIGGGMPVGAFGGRKDVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLNLLREEGN
EKRLASKTKQLANGFKQLADKHGIPMLVHQVGGMFGFFFTDQETVTCYEDVTKCDVERFKRFFHLMLDHGVYLAPSAFEA
SFTSLAHGSKELDATLEAADRSLAIIAAESK
>Mature_430_residues
TKSAELYEKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHVVIRDAVIAAAQR
GLSFGAPTETEIKMAELVSEMVPSMEQLRMVSSGTEATMSAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKLTLTATFNNLDSVREIFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVMT
GFRVAEGCAQAYYNIKPDLTCLGKVIGGGMPVGAFGGRKDVMQYIAPTGPVYQAGTLSGNPVAMAAGYACLNLLREEGNE
KRLASKTKQLANGFKQLADKHGIPMLVHQVGGMFGFFFTDQETVTCYEDVTKCDVERFKRFFHLMLDHGVYLAPSAFEAS
FTSLAHGSKELDATLEAADRSLAIIAAESK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=27.9461279461279, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI37574042, Length=346, Percent_Identity=26.5895953757225, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442705, Length=348, Percent_Identity=25.8620689655172, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226442709, Length=285, Percent_Identity=27.3684210526316, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=29.8578199052133, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=70.9905660377358, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=360, Percent_Identity=28.8888888888889, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI145693181, Length=354, Percent_Identity=29.9435028248588, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788044, Length=318, Percent_Identity=29.874213836478, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787560, Length=354, Percent_Identity=30.225988700565, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789759, Length=327, Percent_Identity=25.9938837920489, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786991, Length=390, Percent_Identity=27.4358974358974, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71992977, Length=358, Percent_Identity=25.1396648044693, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=33.5664335664336, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=387, Percent_Identity=27.906976744186, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=351, Percent_Identity=24.2165242165242, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357415, Length=296, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46065; Mature: 45934

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAELYEKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGPLIFDADGKAYIDYVGSWG
CCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEECCCCCEEEEEECCCC
PMILGHNHVVIRDAVIAAAQRGLSFGAPTETEIKMAELVSEMVPSMEQLRMVSSGTEATM
CEEECCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKLTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCHHEEEEEEE
FNNLDSVREIFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
TGFRVAEGCAQAYYNIKPDLTCLGKVIGGGMPVGAFGGRKDVMQYIAPTGPVYQAGTLSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLNLLREEGNEKRLASKTKQLANGFKQLADKHGIPMLVHQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEC
DQETVTCYEDVTKCDVERFKRFFHLMLDHGVYLAPSAFEASFTSLAHGSKELDATLEAAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHCC
RSLAIIAAESK
CEEEEEEECCC
>Mature Secondary Structure 
TKSAELYEKAQQTIPGGVNSPVRAFNGVGGSPIFVERADGPLIFDADGKAYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCEEECCCCCEEEEEECCCC
PMILGHNHVVIRDAVIAAAQRGLSFGAPTETEIKMAELVSEMVPSMEQLRMVSSGTEATM
CEEECCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGFTGRDKILKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKLTLTAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCHHEEEEEEE
FNNLDSVREIFAANKGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
TGFRVAEGCAQAYYNIKPDLTCLGKVIGGGMPVGAFGGRKDVMQYIAPTGPVYQAGTLSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLNLLREEGNEKRLASKTKQLANGFKQLADKHGIPMLVHQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEC
DQETVTCYEDVTKCDVERFKRFFHLMLDHGVYLAPSAFEASFTSLAHGSKELDATLEAAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHCC
RSLAIIAAESK
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA