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The map label for this gene is tyrS [H]

Identifier: 218710458

GI number: 218710458

Start: 2669815

End: 2671002

Strand: Reverse

Name: tyrS [H]

Synonym: VS_2497

Alternate gene names: 218710458

Gene position: 2671002-2669815 (Counterclockwise)

Preceding gene: 218710460

Following gene: 218710457

Centisome position: 80.96

GC content: 45.71

Gene sequence:

>1188_bases
ATGGCGAGTATTGAAGCTGCACTAGCCGAGATCAAACGTGGCGTAGAAGAACTGATTCCAGAAGACGAACTGATTGCGAA
ACTAAAAGAAGGTCGTCCTTTACGCATTAAACTGGGTGCCGATCCAACCGCTCCAGATATCCACCTAGGCCATACGGTTA
TCTTTAACAAGCTTCGTGCTTTCCAAGAGCTTGGTCATGAAGTGACGTTCCTTATCGGTGATTTCACAGCAATGGTTGGT
GATCCATCAGGTAAGAACTCAACACGTCCACCGCTAAGCCGTGAAGACGTATTGAAGAATGCTGAAACTTACAAAGAGCA
AGTATTCAAGATTCTAGATCCTGCGAAAACGCAAATTCGTTTCAACTCTGAGTGGTTATCTGAGCTTGGTGCAGAAGGCA
TGATTCGTCTTGCTTCTAACCAAACTGTAGCTCGTATGCTTGAGCGTGATGACTTTAAAAAGCGTTACGCTGGTGGTCAA
CCGATCGCAATCCACGAATTCATGTACCCACTTCTTCAAGGTCACGACTCTGTTGCACTAGAGAGCGATGTTGAGCTTGG
CGGTACTGACCAGAAGTTTAACCTTCTAATGGGTCGTGAACTGCAAAAAGCTGCAGGTCAAAAACCACAAGCGGTACTAA
TGATGCCACTACTTGTTGGTTTAGACGGCGTTAAGAAGATGTCTAAGTCAGCGCACAACTACATCGGTATTAGTGAAGCA
CCAAGCGAGATGTTTGGTAAGATCATGTCTATCTCTGACGATCTAATGTGGAGCTACTACGAGCTACTGTCTTTCCGTCC
TCTTGAAGAAGTTGCGGAACTGAAGGCTGGCGTTGAGGCGGGCAAAAACCCTCGTGACGTGAAAGTACTTCTTGCTAAAG
AGATCATTGCTCGTTTCCACAGCGAAGCCGATGCTGAAGCGGCTGAGCAAGAGTTCGTTAACCGTTTTGCTAAGAACCAA
GTTCCTGATGAAATGCCTGAATTTGAATTCGAAGCAGGTCTGCCAATTGCGAACGTTCTAAAGGAAGCAGGTCTTGTAAA
CTCGACTTCTGATGCGATGCGTATGATCAAGCAAGGCGCTGCTAAGCTTGAAGGCGAGAAGATTGAAGACAGTAAATTCG
TACCTGAAGCAGGTACTGCGGTTTACCAAGTAGGTAAGCGTAAGTTTGCTCGTATTACTATTAAGTAA

Upstream 100 bases:

>100_bases
AATAGACGACGGAATAGTGTAACTGGTTTCAAAATACATCGCTATTCAAGTAAGATGTCTAATTATTATATTTTTGCCAA
ATCTGTGGGAGTGAACAAGA

Downstream 100 bases:

>100_bases
TATTAAATCTCAATGATTTATAGAAAAGGCATCTTCGGATGCCTTTTTTATTGACAGAAAGTTGACGAAGAATTTTTTAC
AGGATTGCTACACTATGCGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MASIEAALAEIKRGVEELIPEDELIAKLKEGRPLRIKLGADPTAPDIHLGHTVIFNKLRAFQELGHEVTFLIGDFTAMVG
DPSGKNSTRPPLSREDVLKNAETYKEQVFKILDPAKTQIRFNSEWLSELGAEGMIRLASNQTVARMLERDDFKKRYAGGQ
PIAIHEFMYPLLQGHDSVALESDVELGGTDQKFNLLMGRELQKAAGQKPQAVLMMPLLVGLDGVKKMSKSAHNYIGISEA
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAELKAGVEAGKNPRDVKVLLAKEIIARFHSEADAEAAEQEFVNRFAKNQ
VPDEMPEFEFEAGLPIANVLKEAGLVNSTSDAMRMIKQGAAKLEGEKIEDSKFVPEAGTAVYQVGKRKFARITIK

Sequences:

>Translated_395_residues
MASIEAALAEIKRGVEELIPEDELIAKLKEGRPLRIKLGADPTAPDIHLGHTVIFNKLRAFQELGHEVTFLIGDFTAMVG
DPSGKNSTRPPLSREDVLKNAETYKEQVFKILDPAKTQIRFNSEWLSELGAEGMIRLASNQTVARMLERDDFKKRYAGGQ
PIAIHEFMYPLLQGHDSVALESDVELGGTDQKFNLLMGRELQKAAGQKPQAVLMMPLLVGLDGVKKMSKSAHNYIGISEA
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAELKAGVEAGKNPRDVKVLLAKEIIARFHSEADAEAAEQEFVNRFAKNQ
VPDEMPEFEFEAGLPIANVLKEAGLVNSTSDAMRMIKQGAAKLEGEKIEDSKFVPEAGTAVYQVGKRKFARITIK
>Mature_394_residues
ASIEAALAEIKRGVEELIPEDELIAKLKEGRPLRIKLGADPTAPDIHLGHTVIFNKLRAFQELGHEVTFLIGDFTAMVGD
PSGKNSTRPPLSREDVLKNAETYKEQVFKILDPAKTQIRFNSEWLSELGAEGMIRLASNQTVARMLERDDFKKRYAGGQP
IAIHEFMYPLLQGHDSVALESDVELGGTDQKFNLLMGRELQKAAGQKPQAVLMMPLLVGLDGVKKMSKSAHNYIGISEAP
SEMFGKIMSISDDLMWSYYELLSFRPLEEVAELKAGVEAGKNPRDVKVLLAKEIIARFHSEADAEAAEQEFVNRFAKNQV
PDEMPEFEFEAGLPIANVLKEAGLVNSTSDAMRMIKQGAAKLEGEKIEDSKFVPEAGTAVYQVGKRKFARITIK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=424, Percent_Identity=25.4716981132075, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1787925, Length=409, Percent_Identity=26.4058679706601, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=227, Percent_Identity=30.8370044052863, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=415, Percent_Identity=24.578313253012, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=24.317617866005, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 43803; Mature: 43672

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIEAALAEIKRGVEELIPEDELIAKLKEGRPLRIKLGADPTAPDIHLGHTVIFNKLRA
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQELGHEVTFLIGDFTAMVGDPSGKNSTRPPLSREDVLKNAETYKEQVFKILDPAKTQIR
HHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
FNSEWLSELGAEGMIRLASNQTVARMLERDDFKKRYAGGQPIAIHEFMYPLLQGHDSVAL
ECHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEE
ESDVELGGTDQKFNLLMGRELQKAAGQKPQAVLMMPLLVGLDGVKKMSKSAHNYIGISEA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAELKAGVEAGKNPRDVKVLLAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SEADAEAAEQEFVNRFAKNQVPDEMPEFEFEAGLPIANVLKEAGLVNSTSDAMRMIKQGA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
AKLEGEKIEDSKFVPEAGTAVYQVGKRKFARITIK
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
ASIEAALAEIKRGVEELIPEDELIAKLKEGRPLRIKLGADPTAPDIHLGHTVIFNKLRA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQELGHEVTFLIGDFTAMVGDPSGKNSTRPPLSREDVLKNAETYKEQVFKILDPAKTQIR
HHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
FNSEWLSELGAEGMIRLASNQTVARMLERDDFKKRYAGGQPIAIHEFMYPLLQGHDSVAL
ECHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEE
ESDVELGGTDQKFNLLMGRELQKAAGQKPQAVLMMPLLVGLDGVKKMSKSAHNYIGISEA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSEMFGKIMSISDDLMWSYYELLSFRPLEEVAELKAGVEAGKNPRDVKVLLAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SEADAEAAEQEFVNRFAKNQVPDEMPEFEFEAGLPIANVLKEAGLVNSTSDAMRMIKQGA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
AKLEGEKIEDSKFVPEAGTAVYQVGKRKFARITIK
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA