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The map label for this gene is proS [H]

Identifier: 218710332

GI number: 218710332

Start: 2535492

End: 2537207

Strand: Direct

Name: proS [H]

Synonym: VS_2368

Alternate gene names: 218710332

Gene position: 2535492-2537207 (Clockwise)

Preceding gene: 218710331

Following gene: 218710333

Centisome position: 76.85

GC content: 46.79

Gene sequence:

>1716_bases
ATGCGTACCAGTAACTACCTTCTTTCTACTCTGAAAGAGACTCCAAACGACGCAGAAGTTATCAGCCACCAGCTGATGCT
ACGTGCAGGTATGATCCGTAAGCTAGCTTCAGGTTTATATACTTGGCTACCTACTGGTCTACGTGTACTGCGTAAAGTCG
AAAATATCGTTCGCCAAGAGATCGATAATGCAGGTGCCGTTGAAATCTTGATGCCCGTAGTTCAACCGTTTGAGCTTTGG
GAAGAGACTGGCCGTTCTGAAAAGATGGGTCCTGAGCTACTTCGTTTCACAGACCGTCATTCTCGTCCGTTTGTTCTTAG
CCCAACAGCAGAAGAAGTGGTAACGAGCCTAGTACGTAACGAGATCAGCTCTTACAAACAGCTTCCTCTAAATCTGTACC
AAATCCAGACTAAATTCCGTGATGAACGCCGCCCTCGTTTTGGCGTAATGCGTGCTCGTGAATTCTCTATGATGGATGCG
TACAGCTTTGATATCGATAAAGAAGGCTTAGAAAAGTCTTACCAAGCGATGCACGATGCTTACTGTAAAGCATTCGACCG
CATGGGTCTTGAGTACCGTCCAGTATTGGCAGACTCTGGCGCAATCGGTGGCAGCGGCTCTCAAGAGTTCCACGTTCTTG
CTGAAAGCGGCGAAGACCTAATCGCATTCTCTTCAGAATCTGATTACGCAGCGAACATCGAGAAAGCAGAAGCACTCGCT
CCTACTGAAGAAGTTGCAGCGCCAACTCAAGAGATGGAACTGGTTGATACGCCAAACGCAAAAACTATCGCAGAACTTGT
AGAGCAACACGGTCTAGCAATCGAGAAGACAGTTAAGACTCTATTCGTTAAAGCATCTGATGAAGTAGAAGCAGATATCA
TTGCTCTAATCGTCCGTGGCGACCACGAGCTTAACGAAGTGAAAGCAGAAAACCTTCCACAGATTGCTTCTCCACTAGAG
ATGGCCTCTGAAGAAGAAATCCGTGCACTTGTTGGTGCAGGTCCTGGTTCACTTGGCCCTGTTGGCCTAGAGCTGCCATT
CATCGCTGACCGCTCTGTTGCTGTAATGAGTGACTTCGGCGCTGGCGCAAACGTAGACGGTAAGCACTACTTCGGTATTA
ACTGGGGTCGTGACGTTGAGCTTGCTCAAGTTGAAGACTTACGTAACGTTGTTGAAGGCGACCTAAGCCCATGTGGTCAA
GGTACTATCCAACTTAAACGCGGTATCGAAGTTGGTCACATCTTCCAGCTAGGTAACACTTACTCGAAAGCAATGAACTG
TAACGTGCTTGGTCCTGATGGTAAGAGCGTAATCCTAGAAATGGGTTGTTACGGTATCGGTGTTTCACGTGTTGTTGCAT
CTGCTATCGAGCAAAACCACGATAAATTCGGTATCACTTGGCCAGATGCACTAGCACCATTCCAAGTTGCTATCGTACCA
ATGAACATGCACAAATCTGAGCGCGTTAAAGAAGCTGCTGAGAAGCTATACGCTGAATTAACAGCTATGGGAATCGAAGT
ACTATTTGATGATCGTAAAGAGCGCCCAGGTGTTATGTTTAAAGATATCGAGCTTGTGGGTATTCCTCACACTATCGTTA
TCGGTGATCGCAGCATGGACGAAGGTAACTTCGAATACAAAAACCGTCGTACAGGTGATAAAGAAGCTATCGCTATGGAC
ACGGTTATCGAGCACCTTAAAGCTCAACTGGCTTAG

Upstream 100 bases:

>100_bases
ATCCCAAATAGGCTGATATTATATGCGGCTATATCAGATTTGCTGTCGTCACTAACTGCAGCAAATTTTCTTTTATCAAT
GATGAAACGGATACCATAGA

Downstream 100 bases:

>100_bases
TAATCCAATTACTGACTAGATAAACATTGAAAGGCTACCATTAGGTGGCCTTTTTTGTGCCTGTCGTTTCTCTTCAAACT
CCCTTCTATTTATCACCAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSNYLLSTLKETPNDAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAVEILMPVVQPFELW
EETGRSEKMGPELLRFTDRHSRPFVLSPTAEEVVTSLVRNEISSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFSMMDA
YSFDIDKEGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSSESDYAANIEKAEALA
PTEEVAAPTQEMELVDTPNAKTIAELVEQHGLAIEKTVKTLFVKASDEVEADIIALIVRGDHELNEVKAENLPQIASPLE
MASEEEIRALVGAGPGSLGPVGLELPFIADRSVAVMSDFGAGANVDGKHYFGINWGRDVELAQVEDLRNVVEGDLSPCGQ
GTIQLKRGIEVGHIFQLGNTYSKAMNCNVLGPDGKSVILEMGCYGIGVSRVVASAIEQNHDKFGITWPDALAPFQVAIVP
MNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELVGIPHTIVIGDRSMDEGNFEYKNRRTGDKEAIAMD
TVIEHLKAQLA

Sequences:

>Translated_571_residues
MRTSNYLLSTLKETPNDAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAVEILMPVVQPFELW
EETGRSEKMGPELLRFTDRHSRPFVLSPTAEEVVTSLVRNEISSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFSMMDA
YSFDIDKEGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSSESDYAANIEKAEALA
PTEEVAAPTQEMELVDTPNAKTIAELVEQHGLAIEKTVKTLFVKASDEVEADIIALIVRGDHELNEVKAENLPQIASPLE
MASEEEIRALVGAGPGSLGPVGLELPFIADRSVAVMSDFGAGANVDGKHYFGINWGRDVELAQVEDLRNVVEGDLSPCGQ
GTIQLKRGIEVGHIFQLGNTYSKAMNCNVLGPDGKSVILEMGCYGIGVSRVVASAIEQNHDKFGITWPDALAPFQVAIVP
MNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELVGIPHTIVIGDRSMDEGNFEYKNRRTGDKEAIAMD
TVIEHLKAQLA
>Mature_571_residues
MRTSNYLLSTLKETPNDAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAVEILMPVVQPFELW
EETGRSEKMGPELLRFTDRHSRPFVLSPTAEEVVTSLVRNEISSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFSMMDA
YSFDIDKEGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSSESDYAANIEKAEALA
PTEEVAAPTQEMELVDTPNAKTIAELVEQHGLAIEKTVKTLFVKASDEVEADIIALIVRGDHELNEVKAENLPQIASPLE
MASEEEIRALVGAGPGSLGPVGLELPFIADRSVAVMSDFGAGANVDGKHYFGINWGRDVELAQVEDLRNVVEGDLSPCGQ
GTIQLKRGIEVGHIFQLGNTYSKAMNCNVLGPDGKSVILEMGCYGIGVSRVVASAIEQNHDKFGITWPDALAPFQVAIVP
MNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELVGIPHTIVIGDRSMDEGNFEYKNRRTGDKEAIAMD
TVIEHLKAQLA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.0353982300885, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=70.8771929824561, Blast_Score=844, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=235, Percent_Identity=39.1489361702128, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=41.5730337078652, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=201, Percent_Identity=40.7960199004975, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24656200, Length=224, Percent_Identity=37.5, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63178; Mature: 63178

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSNYLLSTLKETPNDAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAVEILMPVVQPFELWEETGRSEKMGPELLRFTDRHSRPFVLSPTAEEVVTSLVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EISSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFSMMDAYSFDIDKEGLEKSYQAMHDA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSSESDYAANIEKAEALA
HHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHCC
PTEEVAAPTQEMELVDTPNAKTIAELVEQHGLAIEKTVKTLFVKASDEVEADIIALIVRG
CHHHHCCCHHHCEEECCCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEEEC
DHELNEVKAENLPQIASPLEMASEEEIRALVGAGPGSLGPVGLELPFIADRSVAVMSDFG
CCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEEECCC
AGANVDGKHYFGINWGRDVELAQVEDLRNVVEGDLSPCGQGTIQLKRGIEVGHIFQLGNT
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHEEECCCH
YSKAMNCNVLGPDGKSVILEMGCYGIGVSRVVASAIEQNHDKFGITWPDALAPFQVAIVP
HHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELVGIPHTIVIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCEEEEECCCCCC
EGNFEYKNRRTGDKEAIAMDTVIEHLKAQLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSNYLLSTLKETPNDAEVISHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAVEILMPVVQPFELWEETGRSEKMGPELLRFTDRHSRPFVLSPTAEEVVTSLVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EISSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFSMMDAYSFDIDKEGLEKSYQAMHDA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSSESDYAANIEKAEALA
HHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHCC
PTEEVAAPTQEMELVDTPNAKTIAELVEQHGLAIEKTVKTLFVKASDEVEADIIALIVRG
CHHHHCCCHHHCEEECCCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEEEC
DHELNEVKAENLPQIASPLEMASEEEIRALVGAGPGSLGPVGLELPFIADRSVAVMSDFG
CCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEEECCC
AGANVDGKHYFGINWGRDVELAQVEDLRNVVEGDLSPCGQGTIQLKRGIEVGHIFQLGNT
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHEEECCCH
YSKAMNCNVLGPDGKSVILEMGCYGIGVSRVVASAIEQNHDKFGITWPDALAPFQVAIVP
HHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSERVKEAAEKLYAELTAMGIEVLFDDRKERPGVMFKDIELVGIPHTIVIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCEEEEECCCCCC
EGNFEYKNRRTGDKEAIAMDTVIEHLKAQLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA