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The map label for this gene is ybfF [H]

Identifier: 218710221

GI number: 218710221

Start: 2422440

End: 2423201

Strand: Direct

Name: ybfF [H]

Synonym: VS_2254

Alternate gene names: 218710221

Gene position: 2422440-2423201 (Clockwise)

Preceding gene: 218710218

Following gene: 218710222

Centisome position: 73.42

GC content: 45.41

Gene sequence:

>762_bases
ATGTCAGTACAGCTCAACTATAAAATTGAAGGTGAGGGTCACACCATTGTTTTGATCCATGGATTATTCGGAAATTTGGA
CAACCTTGGCTTGCTCGCTAGGGATCTAAAAGCCGATCATCAGGTACTTAGCATCGATCTACGTAACCACGGTCAATCTT
TCCATAATGACACTCATAATTATCAAGCTATGGCACAAGATGTGGCTCAACTGCTGAATGTTCTAGAGCTAGAAGATGTC
ACTGTGATTGGTCACTCGATGGGTGGCAAGGTAGCGATGGCACTGACACAACACCTGACGCTGCGTAAATTGATTGTCTT
AGATATGGCACCAGTCGCGTACACCCAAAGTCGTCACGACAACGTATTCGCAGGCCTACAAGCTGTGATAGAAGAAAAAC
CGACATCACGCTCAGAGGCGCTTAAGGTCCTCGCAAAACATATCGAAATTGATGGGGTTCGCCAGTTCTTAACGAAATCT
TTGTTCAAATCAGAACAAGGCATCATGGAATGGCGTTTCAATGTTGCCTCGCTATTAAACAACTACCCACAGATTATTGG
TTGGGAACCGATTGACAAAACCTCGGTCAAAACCTTGCTAATCAAGGGTGGTGACTCAGATTACCTGACGGCTGAACATC
AAACCGCCGTTCAACAGCAGTTTTCCAACGCAAAGGCACATGTTATCGCCAATACTGGACATTGGCTACACGCGGAAAAG
CCCGCTGAAGTGCTCCGTGCAATAAGGAAATTCATCACTTAG

Upstream 100 bases:

>100_bases
TTTTCATTCGCTTTTCACAATCTCAATGTTTAAACTCTGAGGGATTATAGCGAGATCCGCGCGGATACTCTACGTCAAAC
CCACCATGAATAAGATAAAA

Downstream 100 bases:

>100_bases
ATAGGTTGATTAGTCGATATAAATGAGAGCTGTGTAATCACAGCAGATTAACTTTATCTCACAACAGTGATATAGTGCGC
CCAAGCAAATTTGGTATATA

Product: esterase YbfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSVQLNYKIEGEGHTIVLIHGLFGNLDNLGLLARDLKADHQVLSIDLRNHGQSFHNDTHNYQAMAQDVAQLLNVLELEDV
TVIGHSMGGKVAMALTQHLTLRKLIVLDMAPVAYTQSRHDNVFAGLQAVIEEKPTSRSEALKVLAKHIEIDGVRQFLTKS
LFKSEQGIMEWRFNVASLLNNYPQIIGWEPIDKTSVKTLLIKGGDSDYLTAEHQTAVQQQFSNAKAHVIANTGHWLHAEK
PAEVLRAIRKFIT

Sequences:

>Translated_253_residues
MSVQLNYKIEGEGHTIVLIHGLFGNLDNLGLLARDLKADHQVLSIDLRNHGQSFHNDTHNYQAMAQDVAQLLNVLELEDV
TVIGHSMGGKVAMALTQHLTLRKLIVLDMAPVAYTQSRHDNVFAGLQAVIEEKPTSRSEALKVLAKHIEIDGVRQFLTKS
LFKSEQGIMEWRFNVASLLNNYPQIIGWEPIDKTSVKTLLIKGGDSDYLTAEHQTAVQQQFSNAKAHVIANTGHWLHAEK
PAEVLRAIRKFIT
>Mature_252_residues
SVQLNYKIEGEGHTIVLIHGLFGNLDNLGLLARDLKADHQVLSIDLRNHGQSFHNDTHNYQAMAQDVAQLLNVLELEDVT
VIGHSMGGKVAMALTQHLTLRKLIVLDMAPVAYTQSRHDNVFAGLQAVIEEKPTSRSEALKVLAKHIEIDGVRQFLTKSL
FKSEQGIMEWRFNVASLLNNYPQIIGWEPIDKTSVKTLLIKGGDSDYLTAEHQTAVQQQFSNAKAHVIANTGHWLHAEKP
AEVLRAIRKFIT

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=265, Percent_Identity=33.2075471698113, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI23200012, Length=257, Percent_Identity=33.4630350194552, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI223941839, Length=258, Percent_Identity=28.6821705426357, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1786902, Length=241, Percent_Identity=47.7178423236515, Blast_Score=234, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17508535, Length=252, Percent_Identity=32.1428571428571, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17507063, Length=258, Percent_Identity=25.968992248062, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321468, Length=265, Percent_Identity=31.6981132075472, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640348, Length=252, Percent_Identity=28.1746031746032, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646055, Length=257, Percent_Identity=23.3463035019455, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28268; Mature: 28137

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQLNYKIEGEGHTIVLIHGLFGNLDNLGLLARDLKADHQVLSIDLRNHGQSFHNDTHN
CEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCCHHCCCCHH
YQAMAQDVAQLLNVLELEDVTVIGHSMGGKVAMALTQHLTLRKLIVLDMAPVAYTQSRHD
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
NVFAGLQAVIEEKPTSRSEALKVLAKHIEIDGVRQFLTKSLFKSEQGIMEWRFNVASLLN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NYPQIIGWEPIDKTSVKTLLIKGGDSDYLTAEHQTAVQQQFSNAKAHVIANTGHWLHAEK
CCCHHCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCEEECCC
PAEVLRAIRKFIT
HHHHHHHHHHHCC
>Mature Secondary Structure 
SVQLNYKIEGEGHTIVLIHGLFGNLDNLGLLARDLKADHQVLSIDLRNHGQSFHNDTHN
EEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCCHHCCCCHH
YQAMAQDVAQLLNVLELEDVTVIGHSMGGKVAMALTQHLTLRKLIVLDMAPVAYTQSRHD
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
NVFAGLQAVIEEKPTSRSEALKVLAKHIEIDGVRQFLTKSLFKSEQGIMEWRFNVASLLN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NYPQIIGWEPIDKTSVKTLLIKGGDSDYLTAEHQTAVQQQFSNAKAHVIANTGHWLHAEK
CCCHHCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCEEECCC
PAEVLRAIRKFIT
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]