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The map label for this gene is seqA [H]

Identifier: 218710220

GI number: 218710220

Start: 2421807

End: 2422346

Strand: Reverse

Name: seqA [H]

Synonym: VS_2253

Alternate gene names: 218710220

Gene position: 2422346-2421807 (Counterclockwise)

Preceding gene: 218710224

Following gene: 218710219

Centisome position: 73.42

GC content: 41.48

Gene sequence:

>540_bases
ATGAAAACAATTGAGGTTGATGAGGACCTATACCGTTTTATTGCGGGTCAGACAGAACGTATTGGCGAAAGCGCTTCAGA
TATTCTGCGCCGCTTGTTACTGGTTGATAACCAAGGCACGGTTCCGATCGAAGAGATCGTTGAGCCAAAAGGTATCGTTG
TTAGCAAAGAGGTAGGCTTTACTCCAGAGAAGTTCGATGGCGTTAAAGAAATGCGCTCACTACTAATATCAGATGAGTTT
GCATCACTAAAGAAAGCCATTGATCGTTTCATGCTTGTGTTATCAACACTGCATAAAATCGACCCTTTAAGCTTTTCAGA
AGCAACTCAAGTAAAAGGCCGCAAGCGTGTTTACTTTGCAGACAACGAAGCGACGTTGTTAGCAAACGGCAACACAACTA
AGCCTAAAGCTATTCCACAAAGTCCTTTTTGGGTTATTACGAATAACAACACAAGCCGAAAAAGACAGATGGTTGAACAG
CTTATGAGCCGCATGAATTTCCAAGCAGAATTGATAGAAAAAGTAACAGGTTCAATTTAA

Upstream 100 bases:

>100_bases
CTGACATTTTTATCTTATTCATGGTGGGTTTGACGTAGAGTATCCGCGCGGATCTCGCTATAATCCCTCAGAGTTTAAAC
ATTGAGATTGTGAAAAGCGA

Downstream 100 bases:

>100_bases
CGAAATCTGATTTAAACCTCATAATATCCATGGCTAAGAAGATATTATGAGGTTTTTTTGTTTTTAAATTTTATATAAGG
ATGTCGATAATGGCTATGCA

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKTIEVDEDLYRFIAGQTERIGESASDILRRLLLVDNQGTVPIEEIVEPKGIVVSKEVGFTPEKFDGVKEMRSLLISDEF
ASLKKAIDRFMLVLSTLHKIDPLSFSEATQVKGRKRVYFADNEATLLANGNTTKPKAIPQSPFWVITNNNTSRKRQMVEQ
LMSRMNFQAELIEKVTGSI

Sequences:

>Translated_179_residues
MKTIEVDEDLYRFIAGQTERIGESASDILRRLLLVDNQGTVPIEEIVEPKGIVVSKEVGFTPEKFDGVKEMRSLLISDEF
ASLKKAIDRFMLVLSTLHKIDPLSFSEATQVKGRKRVYFADNEATLLANGNTTKPKAIPQSPFWVITNNNTSRKRQMVEQ
LMSRMNFQAELIEKVTGSI
>Mature_179_residues
MKTIEVDEDLYRFIAGQTERIGESASDILRRLLLVDNQGTVPIEEIVEPKGIVVSKEVGFTPEKFDGVKEMRSLLISDEF
ASLKKAIDRFMLVLSTLHKIDPLSFSEATQVKGRKRVYFADNEATLLANGNTTKPKAIPQSPFWVITNNNTSRKRQMVEQ
LMSRMNFQAELIEKVTGSI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=184, Percent_Identity=54.3478260869565, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 20236; Mature: 20236

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDEDLYRFIAGQTERIGESASDILRRLLLVDNQGTVPIEEIVEPKGIVVSKEVGF
CCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCCCC
TPEKFDGVKEMRSLLISDEFASLKKAIDRFMLVLSTLHKIDPLSFSEATQVKGRKRVYFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEE
DNEATLLANGNTTKPKAIPQSPFWVITNNNTSRKRQMVEQLMSRMNFQAELIEKVTGSI
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MKTIEVDEDLYRFIAGQTERIGESASDILRRLLLVDNQGTVPIEEIVEPKGIVVSKEVGF
CCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEECCCCC
TPEKFDGVKEMRSLLISDEFASLKKAIDRFMLVLSTLHKIDPLSFSEATQVKGRKRVYFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEE
DNEATLLANGNTTKPKAIPQSPFWVITNNNTSRKRQMVEQLMSRMNFQAELIEKVTGSI
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]