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The map label for this gene is gltA [H]

Identifier: 218710216

GI number: 218710216

Start: 2416737

End: 2418026

Strand: Direct

Name: gltA [H]

Synonym: VS_2249

Alternate gene names: 218710216

Gene position: 2416737-2418026 (Clockwise)

Preceding gene: 218710207

Following gene: 218710218

Centisome position: 73.25

GC content: 45.5

Gene sequence:

>1290_bases
ATGGCGGATAAGAAAGCTACCCTTCATATTGAAGGTCAAGCGCCGATCGAATTGCCGATTACAGAAGGTGTACTTGGTAC
TCCAGTCATCGACGTTCGTACACTGGGTTCTAATGGTTTTTTCACTTTTGATCCTGGTTTTCTTGCCACTGCATCTTGTG
AATCGCAAATCACTTATATTGACGGTGGAAAAGGTATCCTTCTACACCGTGGTTATCCAATTGATCAACTGGCCAATAAC
GCTGATTACTTAGAAGTTTGTTACATTCTTTTATACGGCGAAGCTCCTTCTCGAGCTCAATACGAAGAGTTCAAGACGAC
TGTCACACGTCACACCATGGTTCATGAGCAAATTGCTAGTTTCTTCCACGGCTTCCGCCGTGATGCTCACCCAATGGCTG
TTATGTGTGGTGTGGTAGGTGCGCTTGCTGCGTTCTATCATGATTCATTAGATGTTAATAACGACACACACCGTGAAATT
GCGGCATACCGCCTAATTTCAAAAATGCCAACACTGGCTGCAATGTGTTACAAATATTCTATCGGTCAACCGTTTATCTA
CCCACGCAATGACCTAGGCTACGCAGAAAACTTCCTGCACATGATGTATGCAAATCCATGTGAAGAATATGAAGTAAACC
CTGTTGTTGCTCGCGCAATGGACAAAATCTTTACGTTACACGCAGATCACGAACAAAATGCTTCAACGTCAACGGTTCGT
TTAGCTGGTTCTTCGGGCGCAAACCCATTTGCTTGTATTGCTGCTGGTATCGCTTCTTTGTGGGGTCCGGCTCACGGCGG
TGCTAACGAAGCTTGTCTAATGATGCTTGAAGAGATCGGTAGCGTAGACAACATTGAAGAGTACGTAGCAAAAGCAAAAG
ACAAAGATGACCCATTCCGTTTAATGGGCTTCGGTCACCGTGTTTACAAGAACTACGATCCACGTGCAACGGTAATGCGC
GAAGCGTGCCACGAAGTACTTAAAGAACTGAACATTCAAGATCCACTACTTGACGTAGCAATGGAACTTGAGCGCATCGC
TCTCTCTGATGAGTACTTTGTATCGAAGAAACTATACCCGAACGTAGATTTTTACTCTGGTATCATTCTGAAAGCGATCG
GCATTCCGGTATCAATGTTCACAGTAATCTTCGCGATGTCTCGTACTATCGGTTGGATTGCACACTGGAACGAAATGCAC
AGCGATCCGACGAACCGCATCGGTCGTCCTCGTCAGTTGTACACTGGTGAAGAACAGCGTGATTTCCAAGCTCTACACGA
GCGCGAATAG

Upstream 100 bases:

>100_bases
GCACACAAAGCTGCAGAAAAATTGACTTTCTGACGTAAGACCAGTAAAAAAATGGCACATAAACATCCTGGACGGATTAA
TAATAACAAAGGAGATTGTT

Downstream 100 bases:

>100_bases
TAAAGTTTCGACTTTTCTAATCGATTAAAATCAAAAAGGGTTGATGTATTAACATCAACCCTTTTCTTTTATATGCTCTC
AACTCCGTCATCCTCAAGAG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MADKKATLHIEGQAPIELPITEGVLGTPVIDVRTLGSNGFFTFDPGFLATASCESQITYIDGGKGILLHRGYPIDQLANN
ADYLEVCYILLYGEAPSRAQYEEFKTTVTRHTMVHEQIASFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDVNNDTHREI
AAYRLISKMPTLAAMCYKYSIGQPFIYPRNDLGYAENFLHMMYANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLMMLEEIGSVDNIEEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMR
EACHEVLKELNIQDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTIGWIAHWNEMH
SDPTNRIGRPRQLYTGEEQRDFQALHERE

Sequences:

>Translated_429_residues
MADKKATLHIEGQAPIELPITEGVLGTPVIDVRTLGSNGFFTFDPGFLATASCESQITYIDGGKGILLHRGYPIDQLANN
ADYLEVCYILLYGEAPSRAQYEEFKTTVTRHTMVHEQIASFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDVNNDTHREI
AAYRLISKMPTLAAMCYKYSIGQPFIYPRNDLGYAENFLHMMYANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
LAGSSGANPFACIAAGIASLWGPAHGGANEACLMMLEEIGSVDNIEEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMR
EACHEVLKELNIQDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTIGWIAHWNEMH
SDPTNRIGRPRQLYTGEEQRDFQALHERE
>Mature_428_residues
ADKKATLHIEGQAPIELPITEGVLGTPVIDVRTLGSNGFFTFDPGFLATASCESQITYIDGGKGILLHRGYPIDQLANNA
DYLEVCYILLYGEAPSRAQYEEFKTTVTRHTMVHEQIASFFHGFRRDAHPMAVMCGVVGALAAFYHDSLDVNNDTHREIA
AYRLISKMPTLAAMCYKYSIGQPFIYPRNDLGYAENFLHMMYANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVRL
AGSSGANPFACIAAGIASLWGPAHGGANEACLMMLEEIGSVDNIEEYVAKAKDKDDPFRLMGFGHRVYKNYDPRATVMRE
ACHEVLKELNIQDPLLDVAMELERIALSDEYFVSKKLYPNVDFYSGIILKAIGIPVSMFTVIFAMSRTIGWIAHWNEMHS
DPTNRIGRPRQLYTGEEQRDFQALHERE

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=364, Percent_Identity=26.3736263736264, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786939, Length=424, Percent_Identity=76.4150943396226, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=377, Percent_Identity=31.5649867374005, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17555174, Length=347, Percent_Identity=28.8184438040346, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=345, Percent_Identity=27.8260869565217, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324328, Length=329, Percent_Identity=28.2674772036474, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=370, Percent_Identity=25.4054054054054, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640126, Length=352, Percent_Identity=27.5568181818182, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640124, Length=352, Percent_Identity=27.5568181818182, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356863, Length=402, Percent_Identity=25.8706467661692, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48059; Mature: 47928

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKATLHIEGQAPIELPITEGVLGTPVIDVRTLGSNGFFTFDPGFLATASCESQITYI
CCCCCEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCCEEEECCCCEEECCCCCEEEEE
DGGKGILLHRGYPIDQLANNADYLEVCYILLYGEAPSRAQYEEFKTTVTRHTMVHEQIAS
ECCCEEEEECCCCHHHHCCCCCHHHEEEHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
FFHGFRRDAHPMAVMCGVVGALAAFYHDSLDVNNDTHREIAAYRLISKMPTLAAMCYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHC
IGQPFIYPRNDLGYAENFLHMMYANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLMMLEEIGSVDNIEEYVAKAKDKDDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
LMGFGHRVYKNYDPRATVMREACHEVLKELNIQDPLLDVAMELERIALSDEYFVSKKLYP
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
NVDFYSGIILKAIGIPVSMFTVIFAMSRTIGWIAHWNEMHSDPTNRIGRPRQLYTGEEQR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCCCCHHH
DFQALHERE
HHHHHHCCC
>Mature Secondary Structure 
ADKKATLHIEGQAPIELPITEGVLGTPVIDVRTLGSNGFFTFDPGFLATASCESQITYI
CCCCEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCCEEEECCCCEEECCCCCEEEEE
DGGKGILLHRGYPIDQLANNADYLEVCYILLYGEAPSRAQYEEFKTTVTRHTMVHEQIAS
ECCCEEEEECCCCHHHHCCCCCHHHEEEHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
FFHGFRRDAHPMAVMCGVVGALAAFYHDSLDVNNDTHREIAAYRLISKMPTLAAMCYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHC
IGQPFIYPRNDLGYAENFLHMMYANPCEEYEVNPVVARAMDKIFTLHADHEQNASTSTVR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIASLWGPAHGGANEACLMMLEEIGSVDNIEEYVAKAKDKDDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCH
LMGFGHRVYKNYDPRATVMREACHEVLKELNIQDPLLDVAMELERIALSDEYFVSKKLYP
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCC
NVDFYSGIILKAIGIPVSMFTVIFAMSRTIGWIAHWNEMHSDPTNRIGRPRQLYTGEEQR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCCCCHHH
DFQALHERE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]