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The map label for this gene is menF [H]

Identifier: 218710162

GI number: 218710162

Start: 2341778

End: 2343100

Strand: Reverse

Name: menF [H]

Synonym: VS_2192

Alternate gene names: 218710162

Gene position: 2343100-2341778 (Counterclockwise)

Preceding gene: 218710167

Following gene: 218710161

Centisome position: 71.02

GC content: 48.0

Gene sequence:

>1323_bases
GTGAACGAGGTCGATTTGTCACATTTCCAGCAAACTATCTCCGCTTTGATTGAGCGAGTACAAAATGCCCAAGCAAGTGA
AGTTCGTTGTGTTGAAGTTCTAACGCAAAAACCATCGTTTGCATTTATAGAATGGCTTCATGCTCAACCGCTCTTTCCAA
AGTTCTACTGGCAGTCACGTGATACTCGTGAAGAGGTCGTTGCGCTCGGGCAAATACATACTTTTTCTGATCCCGCTCCC
GCGTACGCAATTCTTGGTGAAGACCAACGAATCTGGGGTGGGCGCTCGTTTGACGGGCACACCGCAAAAAACCGCCGTTG
CATGGAATCGTTTTTCTTCCTTCCTCAGGTTGAACTGCTCCGCTTTGACAAGACTTGGTCGTTAGCCGTCAACTTATCCC
CTGATCGTGTTGCTTCTATTAATGCTCTAAATAAGCTTTCTGTCGAAGCGGCGATATTGGCCCCTTTGTCGGCGCACATT
GAGCAGATTAACCATGTTCCAGAACGAGAGCAGTGGGGCAACCTTGTCGATAAAGTGCTGAAAGGCATTAGCGACGAAGA
GTACAAAAAAGTCGTTTTAGCGCGTAAAACCACATTGCAACTCGATGCGCCAATCTGCGCGGCTCAGTTGCTTAAGGCCA
GCTACCTGCAAAACCATCACAGCTTTCACTTTATGTTGGTATTGGATTCCAAACACAGCTTTATCGGCTCAACGCCAGAG
CGTTTGTACAGTCGATATGGCACTGAGCTTGATACTGAAGCGCTTGCTGGAACCATTGGTCGCGGAGAAAACGCGACAGA
AGATATGGAGTTAGCCAATTGGCTTTCTCAAGACAGTAAAAACCTCAATGAAAACCAGTATGTGGTCGACGACATTATTG
AGCGTCTCACTCCTCATTCTCAAACGGTACACGTTGAAAAAGAGGCGCGCCTAGTGCGTTTGCGTAAGGTGCAACATCTT
AAACGTAATATCCATGCTCAGCTCAATAACGGCGTCAATGGTGTGCAGTTGCTTGCTGCCTTACAGCCGACGGCTGCGGT
AGCGGGTTTACCGCGCAAAGAAGCGATGGATTTCATACTTGAACATGAACCGTTTGCGAGAGGCTGGTATGCAGGCTCTG
TTGGCTATATTAGCCATCAACGCGCTGAATTCTGTGTCGCGATTCGAAGTGCCTTGATTGTAAACGATCAAGTACAACTG
TTTGCGGGTGCCGGGATCGTGCCTGGATCCGTTGCTGAACATGAGTGGCAAGAGTTGAATAAGAAAATGTCGACTCTGCT
CAGCTTAATTTCAGATCACCCATCATTGGGTGTGGCGTCATGA

Upstream 100 bases:

>100_bases
ATCGCTTAAAATCACACGAATGTTCAAAAGATATAGTAGATTCTTGATCTAAGTGGGTGCTGCCCATAAAGTATCAAACT
AATATAATAATAATTTAGAT

Downstream 100 bases:

>100_bases
ACCACGACCAAGCCGTATTAAATAGAGTTTGGTGTAATACGTTACTTGAAGAGTTAGCCCGCAGCGGCGTTGAACATGTT
TGTGTTGCGCCAGGCTCGCG

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNEVDLSHFQQTISALIERVQNAQASEVRCVEVLTQKPSFAFIEWLHAQPLFPKFYWQSRDTREEVVALGQIHTFSDPAP
AYAILGEDQRIWGGRSFDGHTAKNRRCMESFFFLPQVELLRFDKTWSLAVNLSPDRVASINALNKLSVEAAILAPLSAHI
EQINHVPEREQWGNLVDKVLKGISDEEYKKVVLARKTTLQLDAPICAAQLLKASYLQNHHSFHFMLVLDSKHSFIGSTPE
RLYSRYGTELDTEALAGTIGRGENATEDMELANWLSQDSKNLNENQYVVDDIIERLTPHSQTVHVEKEARLVRLRKVQHL
KRNIHAQLNNGVNGVQLLAALQPTAAVAGLPRKEAMDFILEHEPFARGWYAGSVGYISHQRAEFCVAIRSALIVNDQVQL
FAGAGIVPGSVAEHEWQELNKKMSTLLSLISDHPSLGVAS

Sequences:

>Translated_440_residues
MNEVDLSHFQQTISALIERVQNAQASEVRCVEVLTQKPSFAFIEWLHAQPLFPKFYWQSRDTREEVVALGQIHTFSDPAP
AYAILGEDQRIWGGRSFDGHTAKNRRCMESFFFLPQVELLRFDKTWSLAVNLSPDRVASINALNKLSVEAAILAPLSAHI
EQINHVPEREQWGNLVDKVLKGISDEEYKKVVLARKTTLQLDAPICAAQLLKASYLQNHHSFHFMLVLDSKHSFIGSTPE
RLYSRYGTELDTEALAGTIGRGENATEDMELANWLSQDSKNLNENQYVVDDIIERLTPHSQTVHVEKEARLVRLRKVQHL
KRNIHAQLNNGVNGVQLLAALQPTAAVAGLPRKEAMDFILEHEPFARGWYAGSVGYISHQRAEFCVAIRSALIVNDQVQL
FAGAGIVPGSVAEHEWQELNKKMSTLLSLISDHPSLGVAS
>Mature_440_residues
MNEVDLSHFQQTISALIERVQNAQASEVRCVEVLTQKPSFAFIEWLHAQPLFPKFYWQSRDTREEVVALGQIHTFSDPAP
AYAILGEDQRIWGGRSFDGHTAKNRRCMESFFFLPQVELLRFDKTWSLAVNLSPDRVASINALNKLSVEAAILAPLSAHI
EQINHVPEREQWGNLVDKVLKGISDEEYKKVVLARKTTLQLDAPICAAQLLKASYLQNHHSFHFMLVLDSKHSFIGSTPE
RLYSRYGTELDTEALAGTIGRGENATEDMELANWLSQDSKNLNENQYVVDDIIERLTPHSQTVHVEKEARLVRLRKVQHL
KRNIHAQLNNGVNGVQLLAALQPTAAVAGLPRKEAMDFILEHEPFARGWYAGSVGYISHQRAEFCVAIRSALIVNDQVQL
FAGAGIVPGSVAEHEWQELNKKMSTLLSLISDHPSLGVAS

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=414, Percent_Identity=39.8550724637681, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786809, Length=278, Percent_Identity=31.6546762589928, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1787518, Length=275, Percent_Identity=28.7272727272727, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788114, Length=270, Percent_Identity=30, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=196, Percent_Identity=31.6326530612245, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49426; Mature: 49426

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVDLSHFQQTISALIERVQNAQASEVRCVEVLTQKPSFAFIEWLHAQPLFPKFYWQSR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCC
DTREEVVALGQIHTFSDPAPAYAILGEDQRIWGGRSFDGHTAKNRRCMESFFFLPQVELL
CCHHHHHHHHHHEECCCCCCCEEEECCCCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHH
RFDKTWSLAVNLSPDRVASINALNKLSVEAAILAPLSAHIEQINHVPEREQWGNLVDKVL
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KGISDEEYKKVVLARKTTLQLDAPICAAQLLKASYLQNHHSFHFMLVLDSKHSFIGSTPE
HCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
RLYSRYGTELDTEALAGTIGRGENATEDMELANWLSQDSKNLNENQYVVDDIIERLTPHS
HHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QTVHVEKEARLVRLRKVQHLKRNIHAQLNNGVNGVQLLAALQPTAAVAGLPRKEAMDFIL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHH
EHEPFARGWYAGSVGYISHQRAEFCVAIRSALIVNDQVQLFAGAGIVPGSVAEHEWQELN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHH
KKMSTLLSLISDHPSLGVAS
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNEVDLSHFQQTISALIERVQNAQASEVRCVEVLTQKPSFAFIEWLHAQPLFPKFYWQSR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCC
DTREEVVALGQIHTFSDPAPAYAILGEDQRIWGGRSFDGHTAKNRRCMESFFFLPQVELL
CCHHHHHHHHHHEECCCCCCCEEEECCCCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHH
RFDKTWSLAVNLSPDRVASINALNKLSVEAAILAPLSAHIEQINHVPEREQWGNLVDKVL
EECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KGISDEEYKKVVLARKTTLQLDAPICAAQLLKASYLQNHHSFHFMLVLDSKHSFIGSTPE
HCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
RLYSRYGTELDTEALAGTIGRGENATEDMELANWLSQDSKNLNENQYVVDDIIERLTPHS
HHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QTVHVEKEARLVRLRKVQHLKRNIHAQLNNGVNGVQLLAALQPTAAVAGLPRKEAMDFIL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCHHHHHHHHH
EHEPFARGWYAGSVGYISHQRAEFCVAIRSALIVNDQVQLFAGAGIVPGSVAEHEWQELN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHH
KKMSTLLSLISDHPSLGVAS
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]