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The map label for this gene is menD

Identifier: 218710161

GI number: 218710161

Start: 2340057

End: 2341781

Strand: Reverse

Name: menD

Synonym: VS_2191

Alternate gene names: 218710161

Gene position: 2341781-2340057 (Counterclockwise)

Preceding gene: 218710162

Following gene: 218710160

Centisome position: 70.98

GC content: 46.78

Gene sequence:

>1725_bases
ATGAACCACGACCAAGCCGTATTAAATAGAGTTTGGTGTAATACGTTACTTGAAGAGTTAGCCCGCAGCGGCGTTGAACA
TGTTTGTGTTGCGCCAGGCTCGCGCTCAACACCATTAACACTCGAAGCCGAAGCCAATCCAAAACTGACACTGCACACGC
ACTTTGATGAGCGTGGGCTTGGTTTTCTTGCGTTAGGTTTAGCAAAAGCCAGCAATAAGCCAGTTGCCGTGATTGTCACC
TCTGGTACTGCTGTTGCAAACCTTCTTCCTGCGACGGCTGAATCTGGACTGACACGAGAGAAGCTGATTTTGCTGACCTC
TGATCGACCTATCGATTTAGTCGACTGTGGCGCTAATCAAGCGATTCAACAGCAAGGCATCTTTTCTTCCCATGTTGAAA
GTGCTTTAAATCTACCCAGTCCAACCACACAGATTTCATTGAATTGGCTTCTGACATCCGTTGATAACGCGCTAGCGAAA
CAACGCAATGTTGGCGGTGCGATTCATATCAACTGTCCGTTCCCTGAGCCTCTATATTCTGCGAATAGCGCGGAAATGTA
TGCGGAATACACATCCAGTATCTCTGGGTGGAAGTCGGGAACAGCTTGTTACAGCCAAACGTTTTTACCTAATCAAGTGA
ATACACAACCGATTGCTCCAAGTGAGTATCTTGGACGTAAGGGTGTGGTTATTCTCGGTTCGCTCGATATTGAACAAGCG
ACAAAAGCTCAGCAGTTTGCCACTGCATTAGGCTGGCCAGTTTTTTGCGATCCTCAATCAGGTGTCACAAGTGATTGGAA
GCATTATGATCTGTGGATGCAGAGTGACGTAGCTAAAGCGCAACTCAACCAATGTGATTTCATCCTTCAGTTCGGTGAGC
GTGTTGTTTCTAAGCGCCTTAATCATTGGATAAAGTCGCAAGCTTCATCATTCTGCTCGTCACAATACATTGTTGTGTCA
CCAGATACGCACCGAATCAACCAAGATCATCTGCCTCAAACCCATATCGTTGCTGATATCGAGTCATGGGTGTCAGAACA
GCATTTACCTACCTTGTTAGGTCAACATGCAGGTTGGGCTGCACCTTTGGTCGAAATCGCGAATACGGTTCAACAATTGG
CATTGGCACAAATATCCAATAATGACCAACTGACCGAGTTGAGCGTTGCAGTTGACTTGTCGACTAGACTTAAAGACAGA
GAGTTGTTTGTGGGAAACAGCTTGATGGTAAGGCTGGTGGATATGCTGTCATCAATATCTGCGAATCAAGTTTACAGTAA
TCGTGGCGCATCGGGCATTGATGGCTTGGTGGCGACGGCTGCGGGTGTGGTGAAAGCCAACCAAAACCCCTTGATAATGT
TGATTGGCGATACCTCTTTGCTTTACGACCTCAATTCACTGGCTCTGTTTACTCACAATGTAACGCCGATGGTGATTGTT
GTGACTAACAATGATGGCGGTGCGATCTTTGACTTGTTGCCGGTACCAGAGCAGCAAAAACAGTCACTTTATCAAATGCC
TCATGGTTTCAGTTTTGAACATGCCGCTGCGCAATTCCAGCTTGGTTATGCTGCGCCAGAAACCTTGAACGGCTACCAAA
CGATTGTCGAACAACATTTCGAGCAGGGTCAGGGCACCTTGCTCGTTGAAGTTAAGACGCCTCCCGAACAAGCGGCTACT
TTGTTGAAACAATTCAGTGCAATGCTCATCGAGGCATTAGCCTAA

Upstream 100 bases:

>100_bases
TGCCTGGATCCGTTGCTGAACATGAGTGGCAAGAGTTGAATAAGAAAATGTCGACTCTGCTCAGCTTAATTTCAGATCAC
CCATCATTGGGTGTGGCGTC

Downstream 100 bases:

>100_bases
GGACTTAACATGCTTTACTCCAATTATTACCCAGCTGTACAAGAATCTTCTGACAAACCTTTGCTTGTTTTTTTACATGG
TTTACTCGGAAGCGGGGATG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MNHDQAVLNRVWCNTLLEELARSGVEHVCVAPGSRSTPLTLEAEANPKLTLHTHFDERGLGFLALGLAKASNKPVAVIVT
SGTAVANLLPATAESGLTREKLILLTSDRPIDLVDCGANQAIQQQGIFSSHVESALNLPSPTTQISLNWLLTSVDNALAK
QRNVGGAIHINCPFPEPLYSANSAEMYAEYTSSISGWKSGTACYSQTFLPNQVNTQPIAPSEYLGRKGVVILGSLDIEQA
TKAQQFATALGWPVFCDPQSGVTSDWKHYDLWMQSDVAKAQLNQCDFILQFGERVVSKRLNHWIKSQASSFCSSQYIVVS
PDTHRINQDHLPQTHIVADIESWVSEQHLPTLLGQHAGWAAPLVEIANTVQQLALAQISNNDQLTELSVAVDLSTRLKDR
ELFVGNSLMVRLVDMLSSISANQVYSNRGASGIDGLVATAAGVVKANQNPLIMLIGDTSLLYDLNSLALFTHNVTPMVIV
VTNNDGGAIFDLLPVPEQQKQSLYQMPHGFSFEHAAAQFQLGYAAPETLNGYQTIVEQHFEQGQGTLLVEVKTPPEQAAT
LLKQFSAMLIEALA

Sequences:

>Translated_574_residues
MNHDQAVLNRVWCNTLLEELARSGVEHVCVAPGSRSTPLTLEAEANPKLTLHTHFDERGLGFLALGLAKASNKPVAVIVT
SGTAVANLLPATAESGLTREKLILLTSDRPIDLVDCGANQAIQQQGIFSSHVESALNLPSPTTQISLNWLLTSVDNALAK
QRNVGGAIHINCPFPEPLYSANSAEMYAEYTSSISGWKSGTACYSQTFLPNQVNTQPIAPSEYLGRKGVVILGSLDIEQA
TKAQQFATALGWPVFCDPQSGVTSDWKHYDLWMQSDVAKAQLNQCDFILQFGERVVSKRLNHWIKSQASSFCSSQYIVVS
PDTHRINQDHLPQTHIVADIESWVSEQHLPTLLGQHAGWAAPLVEIANTVQQLALAQISNNDQLTELSVAVDLSTRLKDR
ELFVGNSLMVRLVDMLSSISANQVYSNRGASGIDGLVATAAGVVKANQNPLIMLIGDTSLLYDLNSLALFTHNVTPMVIV
VTNNDGGAIFDLLPVPEQQKQSLYQMPHGFSFEHAAAQFQLGYAAPETLNGYQTIVEQHFEQGQGTLLVEVKTPPEQAAT
LLKQFSAMLIEALA
>Mature_574_residues
MNHDQAVLNRVWCNTLLEELARSGVEHVCVAPGSRSTPLTLEAEANPKLTLHTHFDERGLGFLALGLAKASNKPVAVIVT
SGTAVANLLPATAESGLTREKLILLTSDRPIDLVDCGANQAIQQQGIFSSHVESALNLPSPTTQISLNWLLTSVDNALAK
QRNVGGAIHINCPFPEPLYSANSAEMYAEYTSSISGWKSGTACYSQTFLPNQVNTQPIAPSEYLGRKGVVILGSLDIEQA
TKAQQFATALGWPVFCDPQSGVTSDWKHYDLWMQSDVAKAQLNQCDFILQFGERVVSKRLNHWIKSQASSFCSSQYIVVS
PDTHRINQDHLPQTHIVADIESWVSEQHLPTLLGQHAGWAAPLVEIANTVQQLALAQISNNDQLTELSVAVDLSTRLKDR
ELFVGNSLMVRLVDMLSSISANQVYSNRGASGIDGLVATAAGVVKANQNPLIMLIGDTSLLYDLNSLALFTHNVTPMVIV
VTNNDGGAIFDLLPVPEQQKQSLYQMPHGFSFEHAAAQFQLGYAAPETLNGYQTIVEQHFEQGQGTLLVEVKTPPEQAAT
LLKQFSAMLIEALA

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=570, Percent_Identity=42.280701754386, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_VIBSL (B7VHY7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417782.1
- ProteinModelPortal:   B7VHY7
- GeneID:   7161736
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2191
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62325; Mature: 62325

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHDQAVLNRVWCNTLLEELARSGVEHVCVAPGSRSTPLTLEAEANPKLTLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCC
GFLALGLAKASNKPVAVIVTSGTAVANLLPATAESGLTREKLILLTSDRPIDLVDCGANQ
CEEEEEHHHCCCCCEEEEEECCCHHHHHCCHHHHCCCCEEEEEEEECCCCEEEEECCCCH
AIQQQGIFSSHVESALNLPSPTTQISLNWLLTSVDNALAKQRNVGGAIHINCPFPEPLYS
HHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCC
ANSAEMYAEYTSSISGWKSGTACYSQTFLPNQVNTQPIAPSEYLGRKGVVILGSLDIEQA
CCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCHHHH
TKAQQFATALGWPVFCDPQSGVTSDWKHYDLWMQSDVAKAQLNQCDFILQFGERVVSKRL
HHHHHHHHHHCCCEEECCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHWIKSQASSFCSSQYIVVSPDTHRINQDHLPQTHIVADIESWVSEQHLPTLLGQHAGWA
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
APLVEIANTVQQLALAQISNNDQLTELSVAVDLSTRLKDRELFVGNSLMVRLVDMLSSIS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCCCCEEEECCHHHHHHHHHHHHCC
ANQVYSNRGASGIDGLVATAAGVVKANQNPLIMLIGDTSLLYDLNSLALFTHNVTPMVIV
HHHHHHCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCHHHHHHCCEEEEEECCCEEEEE
VTNNDGGAIFDLLPVPEQQKQSLYQMPHGFSFEHAAAQFQLGYAAPETLNGYQTIVEQHF
EEECCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHH
EQGQGTLLVEVKTPPEQAATLLKQFSAMLIEALA
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHDQAVLNRVWCNTLLEELARSGVEHVCVAPGSRSTPLTLEAEANPKLTLHTHFDERGL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCC
GFLALGLAKASNKPVAVIVTSGTAVANLLPATAESGLTREKLILLTSDRPIDLVDCGANQ
CEEEEEHHHCCCCCEEEEEECCCHHHHHCCHHHHCCCCEEEEEEEECCCCEEEEECCCCH
AIQQQGIFSSHVESALNLPSPTTQISLNWLLTSVDNALAKQRNVGGAIHINCPFPEPLYS
HHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCC
ANSAEMYAEYTSSISGWKSGTACYSQTFLPNQVNTQPIAPSEYLGRKGVVILGSLDIEQA
CCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCHHHH
TKAQQFATALGWPVFCDPQSGVTSDWKHYDLWMQSDVAKAQLNQCDFILQFGERVVSKRL
HHHHHHHHHHCCCEEECCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHWIKSQASSFCSSQYIVVSPDTHRINQDHLPQTHIVADIESWVSEQHLPTLLGQHAGWA
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
APLVEIANTVQQLALAQISNNDQLTELSVAVDLSTRLKDRELFVGNSLMVRLVDMLSSIS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCCCCEEEECCHHHHHHHHHHHHCC
ANQVYSNRGASGIDGLVATAAGVVKANQNPLIMLIGDTSLLYDLNSLALFTHNVTPMVIV
HHHHHHCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCHHHHHHCCEEEEEECCCEEEEE
VTNNDGGAIFDLLPVPEQQKQSLYQMPHGFSFEHAAAQFQLGYAAPETLNGYQTIVEQHF
EEECCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHH
EQGQGTLLVEVKTPPEQAATLLKQFSAMLIEALA
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA