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The map label for this gene is menE [H]

Identifier: 218710157

GI number: 218710157

Start: 2335706

End: 2337142

Strand: Reverse

Name: menE [H]

Synonym: VS_2187

Alternate gene names: 218710157

Gene position: 2337142-2335706 (Counterclockwise)

Preceding gene: 218710158

Following gene: 218710155

Centisome position: 70.84

GC content: 46.28

Gene sequence:

>1437_bases
ATGATGCGCCCACAATCTAATCCTCACCCGCTCTGGGTACAGTGGGCGCAGCAGAACCCACATCAAACAGCGTTAGTGAT
TCCTAACCGCGCTTACACTTGGCAGCAAGTGTCTGTGCTGGTGAGTGAGTACCAACAACAGCTATCTCATCAAGGCTTAT
CTGCAGGTGATGTACTGACGATTGTCGGTAAGAACCAAGCTGAAGTGATTCCAGTTTATCTTGCCGCACTCAATTTGGGT
GTGGTTTGTGCGTTTACCATGCCTCAGCCGAAAGCTCGTTTAACGCAGAAGCTTGAATCCCTCTATGGTCAGCTAGGCAG
ACGTTATCTTTGGCTATTAGATAGCTGTGGGTTAGATCATTCTGATGCTGTTGACCTCAAGACTGTATTGGTGACGTTAC
CTTGCTTGAGTGAAGTTAAGGTCGATGCTGATGACAAACCAATAGCAACATCAGAATCTTCTAGTTTCAATCCTCAAAAC
CTTGCAAGCATCGTATTCACTTCTGGTTCTACCGGAAACCCGAAAGCGGTGGCTCACACGTTACAGCAACACTTATGTTC
AGCTCAGGGTTTACTAGACGTTTTCAACTTTGAACAAGCTGACACTTGGTTGCTTAGCTTACCGATGTTCCATGTGTCGG
GATTGGCGATTGTCCATCGTTGGCTGGCGGCTGGTGGCTGCATCAAAATAGGGTCAGGTAAGCTTGAGTCAGACATCGAA
GGTTGCAGTCATGCCTCTTTAGTGGCTACTCAACTACATAGGCTACTAAAGAGTAAGCAAGCACTTACTTTAACTCATGT
GCTTTTAGGCGGTAGCCAGATCCCAGAAGCGCTAGGGCTTGAAGCTCAACAAATGGGCATTGAAACTTGGCTTGGATATG
GCATGACAGAAGCGGCTTCAACAGTGACCGCGAAGCCAGTCGATTCTAGTAATACTGCAGGTTTTGTTCTCGGCCAACGC
CAGTTAAAAATTGAAGATCAGCGTATCTTTATTGGAGGCAATACGCTTGCTTCTGGTTATTACTATCAGGGCGAGTTAAC
ACCGTTGGTGGATGAGAACGGTTGGTTCGACAGTAAAGACCTCGGCGAGTGGGATGGCGAACAAGTGTCGATTATTGGCC
GTGCTGATAATCAGTTTATTTCTGGGGGAGAGAATATCCACTGTGAAGAAATTGAACGAGCACTGAATCAGTTATCTTTA
ATCAATCAGGCGTTTATCGTTCCAATTGAAGATGATGAGTTCGGGTTTCGACCCGTCGCTATTGTGGATTGTACGGATTT
ACCAACCAAAGAATGGTTTGCCGAGCAATTAAATGGCCGTCTGGAACGGTTCAAATTTCCTGTCGAGTACTATCGAATGC
CTCATCAAGAACAGCTAAGCATTAAGGTTTCACGCGCAGGATTGGCACAGTGGTTACAGCAGATACGTTCTAAATAG

Upstream 100 bases:

>100_bases
CTTCCAGTTTCAACGCTTGAACAGCAACAATTGATTTGGTCTTCTTAGGCTGATCGTTCGATTGGCTTAGACCGTTCTCA
ATCTATAACTTGGTTATCTT

Downstream 100 bases:

>100_bases
AACTGAGAACAATAAAATGAAGCGCAATACACGGTGACTAGTCGTAATTGCGCTTCAGGGGGTTGTGATGGTGGTGAAAC
CATCACAAATTTTTAATATT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MMRPQSNPHPLWVQWAQQNPHQTALVIPNRAYTWQQVSVLVSEYQQQLSHQGLSAGDVLTIVGKNQAEVIPVYLAALNLG
VVCAFTMPQPKARLTQKLESLYGQLGRRYLWLLDSCGLDHSDAVDLKTVLVTLPCLSEVKVDADDKPIATSESSSFNPQN
LASIVFTSGSTGNPKAVAHTLQQHLCSAQGLLDVFNFEQADTWLLSLPMFHVSGLAIVHRWLAAGGCIKIGSGKLESDIE
GCSHASLVATQLHRLLKSKQALTLTHVLLGGSQIPEALGLEAQQMGIETWLGYGMTEAASTVTAKPVDSSNTAGFVLGQR
QLKIEDQRIFIGGNTLASGYYYQGELTPLVDENGWFDSKDLGEWDGEQVSIIGRADNQFISGGENIHCEEIERALNQLSL
INQAFIVPIEDDEFGFRPVAIVDCTDLPTKEWFAEQLNGRLERFKFPVEYYRMPHQEQLSIKVSRAGLAQWLQQIRSK

Sequences:

>Translated_478_residues
MMRPQSNPHPLWVQWAQQNPHQTALVIPNRAYTWQQVSVLVSEYQQQLSHQGLSAGDVLTIVGKNQAEVIPVYLAALNLG
VVCAFTMPQPKARLTQKLESLYGQLGRRYLWLLDSCGLDHSDAVDLKTVLVTLPCLSEVKVDADDKPIATSESSSFNPQN
LASIVFTSGSTGNPKAVAHTLQQHLCSAQGLLDVFNFEQADTWLLSLPMFHVSGLAIVHRWLAAGGCIKIGSGKLESDIE
GCSHASLVATQLHRLLKSKQALTLTHVLLGGSQIPEALGLEAQQMGIETWLGYGMTEAASTVTAKPVDSSNTAGFVLGQR
QLKIEDQRIFIGGNTLASGYYYQGELTPLVDENGWFDSKDLGEWDGEQVSIIGRADNQFISGGENIHCEEIERALNQLSL
INQAFIVPIEDDEFGFRPVAIVDCTDLPTKEWFAEQLNGRLERFKFPVEYYRMPHQEQLSIKVSRAGLAQWLQQIRSK
>Mature_478_residues
MMRPQSNPHPLWVQWAQQNPHQTALVIPNRAYTWQQVSVLVSEYQQQLSHQGLSAGDVLTIVGKNQAEVIPVYLAALNLG
VVCAFTMPQPKARLTQKLESLYGQLGRRYLWLLDSCGLDHSDAVDLKTVLVTLPCLSEVKVDADDKPIATSESSSFNPQN
LASIVFTSGSTGNPKAVAHTLQQHLCSAQGLLDVFNFEQADTWLLSLPMFHVSGLAIVHRWLAAGGCIKIGSGKLESDIE
GCSHASLVATQLHRLLKSKQALTLTHVLLGGSQIPEALGLEAQQMGIETWLGYGMTEAASTVTAKPVDSSNTAGFVLGQR
QLKIEDQRIFIGGNTLASGYYYQGELTPLVDENGWFDSKDLGEWDGEQVSIIGRADNQFISGGENIHCEEIERALNQLSL
INQAFIVPIEDDEFGFRPVAIVDCTDLPTKEWFAEQLNGRLERFKFPVEYYRMPHQEQLSIKVSRAGLAQWLQQIRSK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=467, Percent_Identity=35.7601713062098, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI145693145, Length=496, Percent_Identity=24.1935483870968, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=466, Percent_Identity=26.1802575107296, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=350, Percent_Identity=27.4285714285714, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52795; Mature: 52795

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRPQSNPHPLWVQWAQQNPHQTALVIPNRAYTWQQVSVLVSEYQQQLSHQGLSAGDVLT
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IVGKNQAEVIPVYLAALNLGVVCAFTMPQPKARLTQKLESLYGQLGRRYLWLLDSCGLDH
EECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCC
SDAVDLKTVLVTLPCLSEVKVDADDKPIATSESSSFNPQNLASIVFTSGSTGNPKAVAHT
CCCHHHHHHHHHHCCHHHCEECCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHH
LQQHLCSAQGLLDVFNFEQADTWLLSLPMFHVSGLAIVHRWLAAGGCIKIGSGKLESDIE
HHHHHHHHHCHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHC
GCSHASLVATQLHRLLKSKQALTLTHVLLGGSQIPEALGLEAQQMGIETWLGYGMTEAAS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCHHHHHC
TVTAKPVDSSNTAGFVLGQRQLKIEDQRIFIGGNTLASGYYYQGELTPLVDENGWFDSKD
CEEECCCCCCCCCEEEEECEEEEEECCEEEECCCEEECCEEEECCCCEEECCCCCCCCCC
LGEWDGEQVSIIGRADNQFISGGENIHCEEIERALNQLSLINQAFIVPIEDDEFGFRPVA
CCCCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEE
IVDCTDLPTKEWFAEQLNGRLERFKFPVEYYRMPHQEQLSIKVSRAGLAQWLQQIRSK
EEECCCCCCHHHHHHHHCCHHHHHCCCHHHHCCCCCHHEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMRPQSNPHPLWVQWAQQNPHQTALVIPNRAYTWQQVSVLVSEYQQQLSHQGLSAGDVLT
CCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IVGKNQAEVIPVYLAALNLGVVCAFTMPQPKARLTQKLESLYGQLGRRYLWLLDSCGLDH
EECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCC
SDAVDLKTVLVTLPCLSEVKVDADDKPIATSESSSFNPQNLASIVFTSGSTGNPKAVAHT
CCCHHHHHHHHHHCCHHHCEECCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHH
LQQHLCSAQGLLDVFNFEQADTWLLSLPMFHVSGLAIVHRWLAAGGCIKIGSGKLESDIE
HHHHHHHHHCHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHC
GCSHASLVATQLHRLLKSKQALTLTHVLLGGSQIPEALGLEAQQMGIETWLGYGMTEAAS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCHHHHHC
TVTAKPVDSSNTAGFVLGQRQLKIEDQRIFIGGNTLASGYYYQGELTPLVDENGWFDSKD
CEEECCCCCCCCCEEEEECEEEEEECCEEEECCCEEECCEEEECCCCEEECCCCCCCCCC
LGEWDGEQVSIIGRADNQFISGGENIHCEEIERALNQLSLINQAFIVPIEDDEFGFRPVA
CCCCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEE
IVDCTDLPTKEWFAEQLNGRLERFKFPVEYYRMPHQEQLSIKVSRAGLAQWLQQIRSK
EEECCCCCCHHHHHHHHCCHHHHHCCCHHHHCCCCCHHEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]