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The map label for this gene is nagE [H]

Identifier: 218710156

GI number: 218710156

Start: 2334097

End: 2335560

Strand: Direct

Name: nagE [H]

Synonym: VS_2186

Alternate gene names: 218710156

Gene position: 2334097-2335560 (Clockwise)

Preceding gene: 218710153

Following gene: 218710163

Centisome position: 70.75

GC content: 47.54

Gene sequence:

>1464_bases
ATGAATATACTTGGGTATATGCAAAAAGTAGGTAAGGCATTGATGGTTCCCGTAGCCACACTGCCAGCTGCAGCAATCCT
TATGGGGATCGGCTACTGGTTGGATCCTGTTGGTTGGGGTTCTGAAAGTATCCTAGCCGCATTCTTGATCAAATCAGGTG
GTGCGATCATCGACAACATGGCGGTTCTGTTCGCGGTGGGTGTCGCATTTGGTTTAAGCCGTGATAAGAATGGTTCTGCG
GCACTGTCCGGTTTTGTGATGTTCCTTGTCGTGACCACTCTACTGGCTCCAGGCTCAGTTGCTCAATTGATGGATGTTGA
GTTAAGTGAAGTACCAGCGGCATTCGGAAAAATCAGCAACCAATTCGTGGGTATCATTGTCGGTATCATTTCCGCTGAGA
TCTACAACCGCTACTCAACGGTTGAGCTTCCTCAAGCTTTAGCGTTCTTCAGCGGTAAGCGTTTGGTTCCTATCCTTACC
TCAATCGCAGGTATGGCGCTCTCTTTTGCTCTGCTATACATCTGGCCTGCTGTTTACGATGCTCTGATCGTTCTAGGAAT
TAAACTAGAAAGCATGGGCGCTGTGGGTGCCGGTCTGTTTGGTTTCTTCAACCGTTTGTTCCTATCCGTTGGTATGCACC
ACGCACTTTACCCAGTGTTCTGGTTCGACGTTGTAGGTATTAACGATATTCCAAACTTCCTCGGCGGTGCACAATCTATC
GCTAACGGTACGGGTATTCCGGGTAAAACAGGTATGTACCAAGCGGGCTTCTTCCCTATCATGATGTTTGGTCTACCAGC
AGCAGCATTGGCGATGTACCACTGCTCTGATGCGAAAAACAAGAACCAAGTCTTCGCAATCATGCTTGCCGCAGCAATGG
CTTCATTCTTCACTGGCATTACTGAGCCTCTAGAGTTCAGTTTCATGTTCCTAGCACCTGTATTGTTCCTATTGCACGCA
GTGTTAACAGGTTTGTCGCTTTACATTGCAGCAAGCATGGAATGGATGGCAGGCTTCGGCTTCTCTGCAGGCTTTGTTGA
CTTGGTGCTATCAAGCCAAAACCCGTTAGCGACTAACTGGTACATGTTATTAGTTCAAGGTGTGGTGTTCTTCGCGCTTT
ACTACGGTATTTTCCGTTTCGCTATCATCAAGTTCAATCTCCGTACTCCGGGTCGTGGTGAAGAGATGGAAGCAGAAGGC
GAGGCCGAATCTCCAGAAGCGTTAATTAGCCTAACCAACAACTACATCGAAGCTATTGGTGGTGCAGGCAACATTCTAGA
AGTCGACAACTGTATTACTCGCTTACGCTTAACAGTAAAAGACTCGTCAGCAGCAGACAGTGACAAACTGAAAAAACTAG
GTGCTGCAGGCGTCGTTCCTATCGGAAAAGGTGGCCTGCAAGTCATTATCGGTTTAGGTAAGGTAGATAGGGTTGCTGAA
CAAATGAAGAAGGCACTCGCTTAA

Upstream 100 bases:

>100_bases
ATAGCCGCAGTTACTTAAAATCCTGCCTCGATCAATATTTAACTCCATACGTTGACGAGCAGAAACATTAAAAAAATTAA
TTACAAACTTAGGGGCACGT

Downstream 100 bases:

>100_bases
GCTAAACAGCCAGATGCAAAGATAAAAGCCTTAATGACCACATAGAATATTAAAAATTTGTGATGGTTTCACCACCATCA
CAACCCCCTGAAGCGCAATT

Product: putative PTS system N-acetylglucosamine and glucose permease

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MNILGYMQKVGKALMVPVATLPAAAILMGIGYWLDPVGWGSESILAAFLIKSGGAIIDNMAVLFAVGVAFGLSRDKNGSA
ALSGFVMFLVVTTLLAPGSVAQLMDVELSEVPAAFGKISNQFVGIIVGIISAEIYNRYSTVELPQALAFFSGKRLVPILT
SIAGMALSFALLYIWPAVYDALIVLGIKLESMGAVGAGLFGFFNRLFLSVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
ANGTGIPGKTGMYQAGFFPIMMFGLPAAALAMYHCSDAKNKNQVFAIMLAAAMASFFTGITEPLEFSFMFLAPVLFLLHA
VLTGLSLYIAASMEWMAGFGFSAGFVDLVLSSQNPLATNWYMLLVQGVVFFALYYGIFRFAIIKFNLRTPGRGEEMEAEG
EAESPEALISLTNNYIEAIGGAGNILEVDNCITRLRLTVKDSSAADSDKLKKLGAAGVVPIGKGGLQVIIGLGKVDRVAE
QMKKALA

Sequences:

>Translated_487_residues
MNILGYMQKVGKALMVPVATLPAAAILMGIGYWLDPVGWGSESILAAFLIKSGGAIIDNMAVLFAVGVAFGLSRDKNGSA
ALSGFVMFLVVTTLLAPGSVAQLMDVELSEVPAAFGKISNQFVGIIVGIISAEIYNRYSTVELPQALAFFSGKRLVPILT
SIAGMALSFALLYIWPAVYDALIVLGIKLESMGAVGAGLFGFFNRLFLSVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
ANGTGIPGKTGMYQAGFFPIMMFGLPAAALAMYHCSDAKNKNQVFAIMLAAAMASFFTGITEPLEFSFMFLAPVLFLLHA
VLTGLSLYIAASMEWMAGFGFSAGFVDLVLSSQNPLATNWYMLLVQGVVFFALYYGIFRFAIIKFNLRTPGRGEEMEAEG
EAESPEALISLTNNYIEAIGGAGNILEVDNCITRLRLTVKDSSAADSDKLKKLGAAGVVPIGKGGLQVIIGLGKVDRVAE
QMKKALA
>Mature_487_residues
MNILGYMQKVGKALMVPVATLPAAAILMGIGYWLDPVGWGSESILAAFLIKSGGAIIDNMAVLFAVGVAFGLSRDKNGSA
ALSGFVMFLVVTTLLAPGSVAQLMDVELSEVPAAFGKISNQFVGIIVGIISAEIYNRYSTVELPQALAFFSGKRLVPILT
SIAGMALSFALLYIWPAVYDALIVLGIKLESMGAVGAGLFGFFNRLFLSVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
ANGTGIPGKTGMYQAGFFPIMMFGLPAAALAMYHCSDAKNKNQVFAIMLAAAMASFFTGITEPLEFSFMFLAPVLFLLHA
VLTGLSLYIAASMEWMAGFGFSAGFVDLVLSSQNPLATNWYMLLVQGVVFFALYYGIFRFAIIKFNLRTPGRGEEMEAEG
EAESPEALISLTNNYIEAIGGAGNILEVDNCITRLRLTVKDSSAADSDKLKKLGAAGVVPIGKGGLQVIIGLGKVDRVAE
QMKKALA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=501, Percent_Identity=42.1157684630739, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1787343, Length=501, Percent_Identity=35.5289421157685, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1787908, Length=516, Percent_Identity=35.077519379845, Blast_Score=254, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51759; Mature: 51759

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGYMQKVGKALMVPVATLPAAAILMGIGYWLDPVGWGSESILAAFLIKSGGAIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHH
AVLFAVGVAFGLSRDKNGSAALSGFVMFLVVTTLLAPGSVAQLMDVELSEVPAAFGKISN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHH
QFVGIIVGIISAEIYNRYSTVELPQALAFFSGKRLVPILTSIAGMALSFALLYIWPAVYD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIVLGIKLESMGAVGAGLFGFFNRLFLSVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHH
ANGTGIPGKTGMYQAGFFPIMMFGLPAAALAMYHCSDAKNKNQVFAIMLAAAMASFFTGI
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPVLFLLHAVLTGLSLYIAASMEWMAGFGFSAGFVDLVLSSQNPLATNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHH
YMLLVQGVVFFALYYGIFRFAIIKFNLRTPGRGEEMEAEGEAESPEALISLTNNYIEAIG
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GAGNILEVDNCITRLRLTVKDSSAADSDKLKKLGAAGVVPIGKGGLQVIIGLGKVDRVAE
CCCCEEEHHHHHHHHEEEECCCCCCCHHHHHHHCCCCCCEECCCCEEEEEECCHHHHHHH
QMKKALA
HHHHHCC
>Mature Secondary Structure
MNILGYMQKVGKALMVPVATLPAAAILMGIGYWLDPVGWGSESILAAFLIKSGGAIIDNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHH
AVLFAVGVAFGLSRDKNGSAALSGFVMFLVVTTLLAPGSVAQLMDVELSEVPAAFGKISN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHH
QFVGIIVGIISAEIYNRYSTVELPQALAFFSGKRLVPILTSIAGMALSFALLYIWPAVYD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIVLGIKLESMGAVGAGLFGFFNRLFLSVGMHHALYPVFWFDVVGINDIPNFLGGAQSI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHH
ANGTGIPGKTGMYQAGFFPIMMFGLPAAALAMYHCSDAKNKNQVFAIMLAAAMASFFTGI
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
TEPLEFSFMFLAPVLFLLHAVLTGLSLYIAASMEWMAGFGFSAGFVDLVLSSQNPLATNW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHH
YMLLVQGVVFFALYYGIFRFAIIKFNLRTPGRGEEMEAEGEAESPEALISLTNNYIEAIG
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GAGNILEVDNCITRLRLTVKDSSAADSDKLKKLGAAGVVPIGKGGLQVIIGLGKVDRVAE
CCCCEEEHHHHHHHHEEEECCCCCCCHHHHHHHCCCCCCEECCCCEEEEEECCHHHHHHH
QMKKALA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]