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The map label for this gene is gltB [C]

Identifier: 218710117

GI number: 218710117

Start: 2294737

End: 2296299

Strand: Reverse

Name: gltB [C]

Synonym: VS_2138

Alternate gene names: 218710117

Gene position: 2296299-2294737 (Counterclockwise)

Preceding gene: 218710118

Following gene: 218710116

Centisome position: 69.6

GC content: 49.07

Gene sequence:

>1563_bases
ATGAAGAATCCAGTGATAGCGGATACCAAGCCAGTCAAAGTGGAGTTAACAGAAGAGGAGGAGTATTACTTCTGTACCTG
TGGTAAATCGAAAAACCAACCTTACTGCGACGGCTCTCATGCAGGAACCGGCTTCAAACCGAAGAGTTTCGTGGCAGAGG
AAACCGGCGACGCTTACCTGTGCCGCTGTAAGTATTCAAACAACCTCCCTTTTTGTGATGGCACTCATAAACAGTTTACG
GCGGAACAAGTGGGGCAAGAAGGCCCAGAGGTTCACATCCAAGCGGCTGCGCCCGATCTCTCACCTGCGGCTTCTGCAAC
CAAAGAAGAGCCCACCGTCGAGTTCATTCATCAATTAGCACGAGATGGCTTATCCAAAGTGGGTCACCATGGGCCAATGA
CTTCAATGGGTGTACCAAGATATCTATTGCCTCATTGGGATGACATTCAGGTGATGGTTGCTCAAATGGCGACCAAACCT
CTGCTTGAAAATGTGCCTGTGGGTACTGAGTTGATTATTGGGCCCAAAGCGAAAAAGCCGCTCAAATTGAACATCTCGTT
GTTTGTGTCCGATATGAGTTTTGGCTCTTTGTCTGAAGAAGCAAAAGTCTCGTTGGCGACAGGCGCGGAACTCGCAGGGA
CAGGTATCTGTTCTGGAGAGGGCGGTATGTTACCCGAAGAGCAAGCGGCGAACTCACGTTACTTTTACGAACTAGCCAGC
GCACAATTTGGTTACGATGAATCTAAGCTAATAAATGTCCAAGCTTTCCATTTTAAAGGTGGGCAGGGTGCTAAAACCGG
AACAGGAGGGCACCTACCGGGGGCGAAGAATATCGGTAAGATAGCAGAGGTGCGTGGCATTGAAGCGGGTACCGCCGCGA
TTTCTCCACCCACCTTTGTTGATCTAAAAACCGTCGAGGATTTTAAAAAGTTCGCCAACCGAGTTCGAGAGGTGACAGGC
GGAATCCCAATTGGCTTCAAGTTAAGTGCCAATCATATTGAAGAGGATATTCAGTTTGCGCTTGATGCCAGTGCCGATTA
CATCATCTTGGATGGTCGAGGCGGTGGTACTGGGGCGGCGCCAGAAATGTTCCGAGACCATATTAGTGTGCCGACCATTC
CAGCGCTTGCTCGTGCCAGAGCCTATCTAGACAAGCAAGGCGTAAGTGACAGAGTCACCTTAATCATTACTGGTGGGCTT
CGTGTTCCAATGGACTTCGTAAAAGCGATGGCGCTTGGTGCTGATGGAGTCGCCATTTCGAACAGCGCCATGCAGTCGAT
TGGTTGTGTGGCGGCGAGAATGTGTAATACCAACAATTGCCCAGCAGGCATCGCGACTCAAAAAGCCGATTTACGCCAAC
GCTTGAATGTCGAGAAAGCGTCGAATCAACTCAAGAACTTCTTCGAAGCTTCGAATGAATTGATGCAAGTGATGGCGAGA
GCGTGCGGCCATGATCATTTAAATCAGTTTAACTCGCACGATTTAGCGACATGGAATCGAGAAATGGCCGAACTATCTGG
AGTCGCTTACTCTGGTGTCAGTTCACTAAATCAACTTAAGTAA

Upstream 100 bases:

>100_bases
TCATTTATTGAATTCGTCAGGCATCTCACCTTTACTTTACTCTTGTGAGCAAGCCATCGCTTCAGTCATCAAATCATAAG
AAAGAAAAGGTGAACGAACT

Downstream 100 bases:

>100_bases
ATAGTATTTCGATAAAGCACACTCACTGTATTTTGGGTGTGCTTATTTTTGTCTGTGTAATGCCAATCTATGTTTAACCT
TTCGTAATTAACTGCTTTAT

Product: putative glutamate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKNPVIADTKPVKVELTEEEEYYFCTCGKSKNQPYCDGSHAGTGFKPKSFVAEETGDAYLCRCKYSNNLPFCDGTHKQFT
AEQVGQEGPEVHIQAAAPDLSPAASATKEEPTVEFIHQLARDGLSKVGHHGPMTSMGVPRYLLPHWDDIQVMVAQMATKP
LLENVPVGTELIIGPKAKKPLKLNISLFVSDMSFGSLSEEAKVSLATGAELAGTGICSGEGGMLPEEQAANSRYFYELAS
AQFGYDESKLINVQAFHFKGGQGAKTGTGGHLPGAKNIGKIAEVRGIEAGTAAISPPTFVDLKTVEDFKKFANRVREVTG
GIPIGFKLSANHIEEDIQFALDASADYIILDGRGGGTGAAPEMFRDHISVPTIPALARARAYLDKQGVSDRVTLIITGGL
RVPMDFVKAMALGADGVAISNSAMQSIGCVAARMCNTNNCPAGIATQKADLRQRLNVEKASNQLKNFFEASNELMQVMAR
ACGHDHLNQFNSHDLATWNREMAELSGVAYSGVSSLNQLK

Sequences:

>Translated_520_residues
MKNPVIADTKPVKVELTEEEEYYFCTCGKSKNQPYCDGSHAGTGFKPKSFVAEETGDAYLCRCKYSNNLPFCDGTHKQFT
AEQVGQEGPEVHIQAAAPDLSPAASATKEEPTVEFIHQLARDGLSKVGHHGPMTSMGVPRYLLPHWDDIQVMVAQMATKP
LLENVPVGTELIIGPKAKKPLKLNISLFVSDMSFGSLSEEAKVSLATGAELAGTGICSGEGGMLPEEQAANSRYFYELAS
AQFGYDESKLINVQAFHFKGGQGAKTGTGGHLPGAKNIGKIAEVRGIEAGTAAISPPTFVDLKTVEDFKKFANRVREVTG
GIPIGFKLSANHIEEDIQFALDASADYIILDGRGGGTGAAPEMFRDHISVPTIPALARARAYLDKQGVSDRVTLIITGGL
RVPMDFVKAMALGADGVAISNSAMQSIGCVAARMCNTNNCPAGIATQKADLRQRLNVEKASNQLKNFFEASNELMQVMAR
ACGHDHLNQFNSHDLATWNREMAELSGVAYSGVSSLNQLK
>Mature_520_residues
MKNPVIADTKPVKVELTEEEEYYFCTCGKSKNQPYCDGSHAGTGFKPKSFVAEETGDAYLCRCKYSNNLPFCDGTHKQFT
AEQVGQEGPEVHIQAAAPDLSPAASATKEEPTVEFIHQLARDGLSKVGHHGPMTSMGVPRYLLPHWDDIQVMVAQMATKP
LLENVPVGTELIIGPKAKKPLKLNISLFVSDMSFGSLSEEAKVSLATGAELAGTGICSGEGGMLPEEQAANSRYFYELAS
AQFGYDESKLINVQAFHFKGGQGAKTGTGGHLPGAKNIGKIAEVRGIEAGTAAISPPTFVDLKTVEDFKKFANRVREVTG
GIPIGFKLSANHIEEDIQFALDASADYIILDGRGGGTGAAPEMFRDHISVPTIPALARARAYLDKQGVSDRVTLIITGGL
RVPMDFVKAMALGADGVAISNSAMQSIGCVAARMCNTNNCPAGIATQKADLRQRLNVEKASNQLKNFFEASNELMQVMAR
ACGHDHLNQFNSHDLATWNREMAELSGVAYSGVSSLNQLK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI211057411, Length=75, Percent_Identity=49.3333333333333, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI308199519, Length=293, Percent_Identity=33.4470989761092, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=277, Percent_Identity=33.5740072202166, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115534146, Length=77, Percent_Identity=42.8571428571429, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556380, Length=73, Percent_Identity=42.4657534246575, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=301, Percent_Identity=30.2325581395349, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI28574881, Length=295, Percent_Identity=31.864406779661, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24665539, Length=295, Percent_Identity=31.864406779661, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28573970, Length=81, Percent_Identity=46.9135802469136, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 55750; Mature: 55750

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPVIADTKPVKVELTEEEEYYFCTCGKSKNQPYCDGSHAGTGFKPKSFVAEETGDAYL
CCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEE
CRCKYSNNLPFCDGTHKQFTAEQVGQEGPEVHIQAAAPDLSPAASATKEEPTVEFIHQLA
EEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHHHHH
RDGLSKVGHHGPMTSMGVPRYLLPHWDDIQVMVAQMATKPLLENVPVGTELIIGPKAKKP
HHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
LKLNISLFVSDMSFGSLSEEAKVSLATGAELAGTGICSGEGGMLPEEQAANSRYFYELAS
EEEEEEEEEECCCCCCCCHHHEEEEECCCCCCCCEEECCCCCCCCCHHHCCCEEEEEEHH
AQFGYDESKLINVQAFHFKGGQGAKTGTGGHLPGAKNIGKIAEVRGIEAGTAAISPPTFV
HHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEE
DLKTVEDFKKFANRVREVTGGIPIGFKLSANHIEEDIQFALDASADYIILDGRGGGTGAA
EHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
PEMFRDHISVPTIPALARARAYLDKQGVSDRVTLIITGGLRVPMDFVKAMALGADGVAIS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEC
NSAMQSIGCVAARMCNTNNCPAGIATQKADLRQRLNVEKASNQLKNFFEASNELMQVMAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ACGHDHLNQFNSHDLATWNREMAELSGVAYSGVSSLNQLK
HHCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNPVIADTKPVKVELTEEEEYYFCTCGKSKNQPYCDGSHAGTGFKPKSFVAEETGDAYL
CCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEE
CRCKYSNNLPFCDGTHKQFTAEQVGQEGPEVHIQAAAPDLSPAASATKEEPTVEFIHQLA
EEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCHHHHHHHHH
RDGLSKVGHHGPMTSMGVPRYLLPHWDDIQVMVAQMATKPLLENVPVGTELIIGPKAKKP
HHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
LKLNISLFVSDMSFGSLSEEAKVSLATGAELAGTGICSGEGGMLPEEQAANSRYFYELAS
EEEEEEEEEECCCCCCCCHHHEEEEECCCCCCCCEEECCCCCCCCCHHHCCCEEEEEEHH
AQFGYDESKLINVQAFHFKGGQGAKTGTGGHLPGAKNIGKIAEVRGIEAGTAAISPPTFV
HHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEE
DLKTVEDFKKFANRVREVTGGIPIGFKLSANHIEEDIQFALDASADYIILDGRGGGTGAA
EHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
PEMFRDHISVPTIPALARARAYLDKQGVSDRVTLIITGGLRVPMDFVKAMALGADGVAIS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEC
NSAMQSIGCVAARMCNTNNCPAGIATQKADLRQRLNVEKASNQLKNFFEASNELMQVMAR
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ACGHDHLNQFNSHDLATWNREMAELSGVAYSGVSSLNQLK
HHCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]