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The map label for this gene is nemA [H]

Identifier: 218710074

GI number: 218710074

Start: 2247767

End: 2248864

Strand: Reverse

Name: nemA [H]

Synonym: VS_2095

Alternate gene names: 218710074

Gene position: 2248864-2247767 (Counterclockwise)

Preceding gene: 218710075

Following gene: 218710073

Centisome position: 68.16

GC content: 46.9

Gene sequence:

>1098_bases
ATGACAAACGCACTATTTCAACCAATTCAACTTGGTAGTATCGAACTTAAAAACCGTATTGTTATGCCACCGATGACGCG
TTCTCGCGCAACGCAACCTGGCAATTCAGCAAACGATATGATGGCGTCTTACTACGCTCAACGCGCTTCTGCAGGTTTGA
TTATCGCTGAAGGCACACAAATTTCACCTATGGGTCAAGGTTACGCTTGGACTCCGGGTATCTACTCTTATGAGCAGATT
GCGGGTTGGAAAAAAGTCACCGATGCAGTACATGAAAAAGAGGGCGTTATCTTCGCTCAACTTTGGCATGTAGGCCGTGT
AACACACCCAGACAACATTGGTGGTGAACAACCAATTTCGTCTTCTGCACTTAAAGCAGAAAACGTAAAAGTATTCATCG
ATAATGGCACTGATGAGCCGGGTTTTGTGGATGTGGTTGAACCTCGTGAGATGACGAAAGAAGACATCAAAAACGTGGTT
GGTCAATACCACCAAGCGGCTTTGAACGCGATTGAAGCGGGCTTTGATGGTATCGAACTTCACGCCGCGAATGGTTACTT
GATTAACCAATTCATCGACTCTGAAGCAAACAACCGCACTGACGAATACGGTGGCTCAATTGAAAACCGTTTACGTTTCC
TAGGTGAAGTGGTTGAAGCTATGGTCGAAGCGATTGGTGCTGACCGTGTAGGTGTTCGCCTTGCTCCGTTCACATCGTTG
AATGGTACGGTTGACGCTACGCCAGTAGAGACTTACACAGCGGCGGCGGTGCTACTTAACTTATACAACATTGTTTATCT
ACACGTTGCAGAAGTCGATTGGGACGACGCACCTGAAACGCCAAAAGCGTTTAAAGCAGCGGTTCGTAAAGCTTACAATG
GCGTGCTGATTTACGCAGGTAAATACGACAGCGAGCGCGGCGAAAAAGCGGTTGCTGAAGGCGTAACTGATATGGTTGGT
TTCGGTCGTCCATTCGTAGCAAACCCAGATCTACCCGCTCGTATTCAAAATGGTTACCCATTAGCAGCGCACGATCCAAA
CACATTGTTTGGTGGTGCTGAAAAAGGTTTAACGGATTACCCAAAATACGCAGGCTAA

Upstream 100 bases:

>100_bases
CGGGGCCAGTAAAAGGTGGCTCAACGCACATCGCATTTATCACAGACCCAGATGGCTACCAAATCGAACTGATTCAACTG
GGTTAATCGAGGATATAACA

Downstream 100 bases:

>100_bases
AAATAGCTGCCTGAAGCAGAGTATTGAGATAATATCTCGATCCTGTTGAATGTTTATAAAAAGCAACCTTGCAGAGTCGG
GTTGCTTTTTTTGTTATTGG

Product: NADH:flavin oxidoreductases

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTNALFQPIQLGSIELKNRIVMPPMTRSRATQPGNSANDMMASYYAQRASAGLIIAEGTQISPMGQGYAWTPGIYSYEQI
AGWKKVTDAVHEKEGVIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKNVV
GQYHQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAMVEAIGADRVGVRLAPFTSL
NGTVDATPVETYTAAAVLLNLYNIVYLHVAEVDWDDAPETPKAFKAAVRKAYNGVLIYAGKYDSERGEKAVAEGVTDMVG
FGRPFVANPDLPARIQNGYPLAAHDPNTLFGGAEKGLTDYPKYAG

Sequences:

>Translated_365_residues
MTNALFQPIQLGSIELKNRIVMPPMTRSRATQPGNSANDMMASYYAQRASAGLIIAEGTQISPMGQGYAWTPGIYSYEQI
AGWKKVTDAVHEKEGVIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKNVV
GQYHQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAMVEAIGADRVGVRLAPFTSL
NGTVDATPVETYTAAAVLLNLYNIVYLHVAEVDWDDAPETPKAFKAAVRKAYNGVLIYAGKYDSERGEKAVAEGVTDMVG
FGRPFVANPDLPARIQNGYPLAAHDPNTLFGGAEKGLTDYPKYAG
>Mature_364_residues
TNALFQPIQLGSIELKNRIVMPPMTRSRATQPGNSANDMMASYYAQRASAGLIIAEGTQISPMGQGYAWTPGIYSYEQIA
GWKKVTDAVHEKEGVIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKNVVG
QYHQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAMVEAIGADRVGVRLAPFTSLN
GTVDATPVETYTAAAVLLNLYNIVYLHVAEVDWDDAPETPKAFKAAVRKAYNGVLIYAGKYDSERGEKAVAEGVTDMVGF
GRPFVANPDLPARIQNGYPLAAHDPNTLFGGAEKGLTDYPKYAG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=43.5261707988981, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789463, Length=366, Percent_Identity=29.5081967213115, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17559804, Length=212, Percent_Identity=32.0754716981132, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=316, Percent_Identity=29.4303797468354, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=254, Percent_Identity=31.8897637795276, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=220, Percent_Identity=30.4545454545455, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=27.1428571428571, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=242, Percent_Identity=31.404958677686, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=95, Percent_Identity=43.1578947368421, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=388, Percent_Identity=40.7216494845361, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=38.5224274406332, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39590; Mature: 39459

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNALFQPIQLGSIELKNRIVMPPMTRSRATQPGNSANDMMASYYAQRASAGLIIAEGTQ
CCCCCCCCCCCCCEEECCEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCE
ISPMGQGYAWTPGIYSYEQIAGWKKVTDAVHEKEGVIFAQLWHVGRVTHPDNIGGEQPIS
ECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKNVVGQYHQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAMVEAIGADRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDATPVETYTAAAVLLNLYNIVYLHVAEVDWDDAPETPKAFKAAVRKAYNGVLIYAG
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEC
KYDSERGEKAVAEGVTDMVGFGRPFVANPDLPARIQNGYPLAAHDPNTLFGGAEKGLTDY
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEECCCCCCCCCCCCCCCCCC
PKYAG
CCCCC
>Mature Secondary Structure 
TNALFQPIQLGSIELKNRIVMPPMTRSRATQPGNSANDMMASYYAQRASAGLIIAEGTQ
CCCCCCCCCCCCEEECCEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCE
ISPMGQGYAWTPGIYSYEQIAGWKKVTDAVHEKEGVIFAQLWHVGRVTHPDNIGGEQPIS
ECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKNVVGQYHQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAMVEAIGADRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDATPVETYTAAAVLLNLYNIVYLHVAEVDWDDAPETPKAFKAAVRKAYNGVLIYAG
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEC
KYDSERGEKAVAEGVTDMVGFGRPFVANPDLPARIQNGYPLAAHDPNTLFGGAEKGLTDY
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEECCCCCCCCCCCCCCCCCC
PKYAG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]