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The map label for this gene is lolE [H]

Identifier: 218710045

GI number: 218710045

Start: 2216040

End: 2217284

Strand: Reverse

Name: lolE [H]

Synonym: VS_2065

Alternate gene names: 218710045

Gene position: 2217284-2216040 (Counterclockwise)

Preceding gene: 218710046

Following gene: 218710043

Centisome position: 67.2

GC content: 46.99

Gene sequence:

>1245_bases
GTGTTTTCTTCTTTATCTCTATTGATAGGCGGCCGATTTAGCCGGGCGAAACAACGAGACAAAATGGTGTCGTTCATCTC
CTTGTCTTCGACAATTGGTATTGCTGTTGGTGTCGCGGTAATCATTATCGGCTTATCTGCAATGAATGGTTTTGAGCGAG
AGCTTCAATCACGAGTACTTTCTGTCATTCCTCACGGTGAGTTTGAGGGCGTGAATAAACCAGTAACTCGCTGGGAGCAT
GTGATTGAGGAGTCGGTAAAGCACGATAAAGTCGTTGCTGCTGCGCCTTATGTGAAAATTACCGCGCTTGCCGAAAAAGG
CAAAGAGTTGAAAGCAATTGAAGTTCGCGGCGTTGACCCACAACTTGAGCAACAAGTATCGAGCCTATCGAGTTTTATCG
ATAAGCAAGCTTGGAGCGATTTTAAGGCAGGGCAACAACAAATCATTTTGGGCTCTGGTGTGGCCAATGTACTTGGCGCG
AAAGTCGGTGATTACCTGACTCTGATGATCCCGACGGTGAATGGGTCGGTTAAGGTTCAAGCACCTAAACGTGTTCGAGT
CAAAGTGGTTGGCTTGCTGACGCTTAATGGCCAAATTGACCACAGCTTAGCTTTGGTCCCGATCGGCGATGCGCAAGTTT
ACGCAAACCTAGGCGAGGCAGTGACAGGGGTTTCTTTAAAAGTGACCGATGTTCTGAACGCGAACTCTATTGTGCGTGAG
GTGGGTAACCAGCTTGATGTGTATGTGTATCTACGAAGCTGGCAACAGAAGTTTGGCTTCTTGTATCGAGATATTCAGTT
GGTTCGCACCATTATGTATCTGGTGATGGTACTGGTTATTGGTGTTGCTTGTTTCAATATTGTCTCGACATTAATGATGG
CAGTAAAAGACAGAGCATCAGAGATCGCGATTTTAAGAACCATGGGCGCATCGGACGGCCTTGTGAAACGCATTTTCGTT
TGGCAGGGCGTATTCTCCGGTGTGTTAGGTAGTTTGGTGGGCAGTGTAATAGGCGTGTTAGTGGCGCTTAATCTCACGAC
ACTTATCAAAGGGCTTGAAAGGCTAGTTGATCACCAGTTCTTGTCCGGTGACATCTATTTTGTCGACTTCTTGCCATCAC
AACTCGATATGACGGATGTTGTTGTGGTTTCAGGTACAGCGATTGTATTGAGCCTATTGGCGACATGGTACCCAGCATCA
CGAGCAGCCAAGTTAAACCCTGCCTCGGTGCTTAGCTCGAAGTAA

Upstream 100 bases:

>100_bases
TTTGGTTGTGACTCACGATGGTGAACTTGCCGGTAAGATGGATCGCCAACTTCACATGCAAGATGGTTTACTGGTCAACG
TAGAAAAAGAGGAGAGCTAA

Downstream 100 bases:

>100_bases
TGCGCTGAGTAATACTTCATGTAGCATGATAGACAAATGGCATACACAAAAAAGGATCCCTGATGGGATCCTTTTTTTTT
GAATTCAATCTGCATTCTTT

Product: outer membrane-specific lipoprotein transporter subunit LolE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MFSSLSLLIGGRFSRAKQRDKMVSFISLSSTIGIAVGVAVIIIGLSAMNGFERELQSRVLSVIPHGEFEGVNKPVTRWEH
VIEESVKHDKVVAAAPYVKITALAEKGKELKAIEVRGVDPQLEQQVSSLSSFIDKQAWSDFKAGQQQIILGSGVANVLGA
KVGDYLTLMIPTVNGSVKVQAPKRVRVKVVGLLTLNGQIDHSLALVPIGDAQVYANLGEAVTGVSLKVTDVLNANSIVRE
VGNQLDVYVYLRSWQQKFGFLYRDIQLVRTIMYLVMVLVIGVACFNIVSTLMMAVKDRASEIAILRTMGASDGLVKRIFV
WQGVFSGVLGSLVGSVIGVLVALNLTTLIKGLERLVDHQFLSGDIYFVDFLPSQLDMTDVVVVSGTAIVLSLLATWYPAS
RAAKLNPASVLSSK

Sequences:

>Translated_414_residues
MFSSLSLLIGGRFSRAKQRDKMVSFISLSSTIGIAVGVAVIIIGLSAMNGFERELQSRVLSVIPHGEFEGVNKPVTRWEH
VIEESVKHDKVVAAAPYVKITALAEKGKELKAIEVRGVDPQLEQQVSSLSSFIDKQAWSDFKAGQQQIILGSGVANVLGA
KVGDYLTLMIPTVNGSVKVQAPKRVRVKVVGLLTLNGQIDHSLALVPIGDAQVYANLGEAVTGVSLKVTDVLNANSIVRE
VGNQLDVYVYLRSWQQKFGFLYRDIQLVRTIMYLVMVLVIGVACFNIVSTLMMAVKDRASEIAILRTMGASDGLVKRIFV
WQGVFSGVLGSLVGSVIGVLVALNLTTLIKGLERLVDHQFLSGDIYFVDFLPSQLDMTDVVVVSGTAIVLSLLATWYPAS
RAAKLNPASVLSSK
>Mature_414_residues
MFSSLSLLIGGRFSRAKQRDKMVSFISLSSTIGIAVGVAVIIIGLSAMNGFERELQSRVLSVIPHGEFEGVNKPVTRWEH
VIEESVKHDKVVAAAPYVKITALAEKGKELKAIEVRGVDPQLEQQVSSLSSFIDKQAWSDFKAGQQQIILGSGVANVLGA
KVGDYLTLMIPTVNGSVKVQAPKRVRVKVVGLLTLNGQIDHSLALVPIGDAQVYANLGEAVTGVSLKVTDVLNANSIVRE
VGNQLDVYVYLRSWQQKFGFLYRDIQLVRTIMYLVMVLVIGVACFNIVSTLMMAVKDRASEIAILRTMGASDGLVKRIFV
WQGVFSGVLGSLVGSVIGVLVALNLTTLIKGLERLVDHQFLSGDIYFVDFLPSQLDMTDVVVVSGTAIVLSLLATWYPAS
RAAKLNPASVLSSK

Specific function: Part of an ATP-dependent transport system lolCDE responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprote

COG id: COG4591

COG function: function code M; ABC-type transport system, involved in lipoprotein release, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC-4 integral membrane protein family. LolC/E subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787362, Length=414, Percent_Identity=57.0048309178744, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1787360, Length=417, Percent_Identity=28.537170263789, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003838
- InterPro:   IPR011925
- InterPro:   IPR011926 [H]

Pfam domain/function: PF02687 FtsX [H]

EC number: NA

Molecular weight: Translated: 44757; Mature: 44757

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSLSLLIGGRFSRAKQRDKMVSFISLSSTIGIAVGVAVIIIGLSAMNGFERELQSRVL
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SVIPHGEFEGVNKPVTRWEHVIEESVKHDKVVAAAPYVKITALAEKGKELKAIEVRGVDP
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEHHCCCCEEEEEEECCCH
QLEQQVSSLSSFIDKQAWSDFKAGQQQIILGSGVANVLGAKVGDYLTLMIPTVNGSVKVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEEEECCCEEEEE
APKRVRVKVVGLLTLNGQIDHSLALVPIGDAQVYANLGEAVTGVSLKVTDVLNANSIVRE
CCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEHHHCHHHHHHH
VGNQLDVYVYLRSWQQKFGFLYRDIQLVRTIMYLVMVLVIGVACFNIVSTLMMAVKDRAS
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAILRTMGASDGLVKRIFVWQGVFSGVLGSLVGSVIGVLVALNLTTLIKGLERLVDHQF
HEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGDIYFVDFLPSQLDMTDVVVVSGTAIVLSLLATWYPASRAAKLNPASVLSSK
HCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MFSSLSLLIGGRFSRAKQRDKMVSFISLSSTIGIAVGVAVIIIGLSAMNGFERELQSRVL
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
SVIPHGEFEGVNKPVTRWEHVIEESVKHDKVVAAAPYVKITALAEKGKELKAIEVRGVDP
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEHHCCCCEEEEEEECCCH
QLEQQVSSLSSFIDKQAWSDFKAGQQQIILGSGVANVLGAKVGDYLTLMIPTVNGSVKVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEEEECCCEEEEE
APKRVRVKVVGLLTLNGQIDHSLALVPIGDAQVYANLGEAVTGVSLKVTDVLNANSIVRE
CCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEHHHCHHHHHHH
VGNQLDVYVYLRSWQQKFGFLYRDIQLVRTIMYLVMVLVIGVACFNIVSTLMMAVKDRAS
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAILRTMGASDGLVKRIFVWQGVFSGVLGSLVGSVIGVLVALNLTTLIKGLERLVDHQF
HEEHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGDIYFVDFLPSQLDMTDVVVVSGTAIVLSLLATWYPASRAAKLNPASVLSSK
HCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10783239 [H]