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The map label for this gene is purT [H]

Identifier: 218709706

GI number: 218709706

Start: 1844112

End: 1845287

Strand: Direct

Name: purT [H]

Synonym: VS_1717

Alternate gene names: 218709706

Gene position: 1844112-1845287 (Clockwise)

Preceding gene: 218709705

Following gene: 218709708

Centisome position: 55.89

GC content: 47.19

Gene sequence:

>1176_bases
ATGTTTGGTACTGCTACTCGTGAAAATGCTACTCGTGTACTTCTATTAGGTTCAGGTGAACTGGGTAAAGAAGTTGCTAT
TGAGTGCCAACGTTTAGGTTTGGAAGTTATTGCATGTGACCGCTATGCAGACGCACCAGCGATGCAAGTCGCACATCGTA
GCCATGTATTAGACATGTTAGATGGTGATGCACTTCAAGCTATCATCGAACTAGAAAAACCTGATTACGTGGTTCCTGAA
ATCGAAGCTATTGCCACCAGCAAGTTAGTTGAACTAGAAGCACAAGGTTTGAATGTCGTTCCAACTGCGAATGCAACCAA
GCTAACCATGAACCGAGAAGGCATCCGACGTCTAGCCGCAGAGGAGCTAAAACTAAGCACTTCTCCTTACCGCTTCGCAG
ACACCTTTGAAGATTTCTCAGCCGCTGTTGAATTCGTAGGTATGCCTTGTGTTGTTAAGCCAGTAATGAGCTCTTCAGGT
AAAGGTCAAAGCATTATCAAAACAGAAGAAGACATTCAGAAGTCTTGGGACTACGCACAAGAAGGCGGTCGTACTGGTGC
GGGTCGTGTGATCGTTGAAGGCTTCATCGACTTTGATTACGAGATCACGCTTCTAACCGTTCGCGCAGTCGACGGTGTTC
ACTTCTGTGCACCTATCGGTCACCGCCAAGAAGACGGTGATTACCGTGAATCATGGCAGCCACAAGTGATGTCAGACAAC
GCACTTAAAGCCGCGCAATACACGGCTGAACAAGTGGTTAACGCACTAGGTGGTCACGGCATCTTTGGTGTCGAGTTGTT
TGTTAAAGGCGATCACGTAATCTTCAACGAAGTATCCCCTCGCCCACACGATACTGGCTTAGTAACATTGATGTCGCAAG
ACTCATCTGAATTCGCACTGCACGTACGCGCCTTTACCGGTATGCCGATCAAATCTATCACGCAATACGGCCCTTGTGCA
TCAGCGGTTATCCTTGGCCAAGGCACTTCAACCAACATTCGTTTTGAAGGTCTTACAGAGGCGCTAGACGCACCACAAAC
GCAAGTCCGCTTGTTTGGTAAGCCTGATATCGATGGTCGCCGCCGTCTAGGCGTGACATTAACTCGTCGTAATAGTACAG
AAACAGCGATTAAAGATGCCATTGAAAGCGCTGCAAAAGTAAAAGTGATTTACTAA

Upstream 100 bases:

>100_bases
AATTCACACCTCAACTTGCGCAAACGTTTGCTTTTATTCTTCAAATAAGTATGATCACCCCGTTTTCAATCAATCGTTCA
AAAGATCGGAAGGAATTTCT

Downstream 100 bases:

>100_bases
CCTGCTATTCAATTCTGAACAATCACGATTTCAAATCTGAATAATAAAAAAGACGGGCTATCGAGCCCGTCTTTTTGTTG
GTGTTATCTTTTGTATCGAA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFGTATRENATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVLDMLDGDALQAIIELEKPDYVVPE
IEAIATSKLVELEAQGLNVVPTANATKLTMNREGIRRLAAEELKLSTSPYRFADTFEDFSAAVEFVGMPCVVKPVMSSSG
KGQSIIKTEEDIQKSWDYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSDN
ALKAAQYTAEQVVNALGGHGIFGVELFVKGDHVIFNEVSPRPHDTGLVTLMSQDSSEFALHVRAFTGMPIKSITQYGPCA
SAVILGQGTSTNIRFEGLTEALDAPQTQVRLFGKPDIDGRRRLGVTLTRRNSTETAIKDAIESAAKVKVIY

Sequences:

>Translated_391_residues
MFGTATRENATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVLDMLDGDALQAIIELEKPDYVVPE
IEAIATSKLVELEAQGLNVVPTANATKLTMNREGIRRLAAEELKLSTSPYRFADTFEDFSAAVEFVGMPCVVKPVMSSSG
KGQSIIKTEEDIQKSWDYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSDN
ALKAAQYTAEQVVNALGGHGIFGVELFVKGDHVIFNEVSPRPHDTGLVTLMSQDSSEFALHVRAFTGMPIKSITQYGPCA
SAVILGQGTSTNIRFEGLTEALDAPQTQVRLFGKPDIDGRRRLGVTLTRRNSTETAIKDAIESAAKVKVIY
>Mature_391_residues
MFGTATRENATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVLDMLDGDALQAIIELEKPDYVVPE
IEAIATSKLVELEAQGLNVVPTANATKLTMNREGIRRLAAEELKLSTSPYRFADTFEDFSAAVEFVGMPCVVKPVMSSSG
KGQSIIKTEEDIQKSWDYAQEGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSDN
ALKAAQYTAEQVVNALGGHGIFGVELFVKGDHVIFNEVSPRPHDTGLVTLMSQDSSEFALHVRAFTGMPIKSITQYGPCA
SAVILGQGTSTNIRFEGLTEALDAPQTQVRLFGKPDIDGRRRLGVTLTRRNSTETAIKDAIESAAKVKVIY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=70.1799485861183, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1786733, Length=264, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=359, Percent_Identity=24.2339832869081, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42609; Mature: 42609

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTATRENATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVLDML
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DGDALQAIIELEKPDYVVPEIEAIATSKLVELEAQGLNVVPTANATKLTMNREGIRRLAA
CCHHHHHHHEECCCCEECCCHHHHHHHHHEEEECCCCEEEECCCCEEEEECHHHHHHHHH
EELKLSTSPYRFADTFEDFSAAVEFVGMPCVVKPVMSSSGKGQSIIKTEEDIQKSWDYAQ
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEEECCHHHHHHHHHHHH
EGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSDN
HCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCCC
ALKAAQYTAEQVVNALGGHGIFGVELFVKGDHVIFNEVSPRPHDTGLVTLMSQDSSEFAL
CHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCCEEE
HVRAFTGMPIKSITQYGPCASAVILGQGTSTNIRFEGLTEALDAPQTQVRLFGKPDIDGR
EEEEECCCCHHHHHHCCCCCCEEEEECCCCCEEEECCHHHHHCCCCEEEEEECCCCCCCC
RRLGVTLTRRNSTETAIKDAIESAAKVKVIY
EEEEEEEEECCCCHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MFGTATRENATRVLLLGSGELGKEVAIECQRLGLEVIACDRYADAPAMQVAHRSHVLDML
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DGDALQAIIELEKPDYVVPEIEAIATSKLVELEAQGLNVVPTANATKLTMNREGIRRLAA
CCHHHHHHHEECCCCEECCCHHHHHHHHHEEEECCCCEEEECCCCEEEEECHHHHHHHHH
EELKLSTSPYRFADTFEDFSAAVEFVGMPCVVKPVMSSSGKGQSIIKTEEDIQKSWDYAQ
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEEECCHHHHHHHHHHHH
EGGRTGAGRVIVEGFIDFDYEITLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMSDN
HCCCCCCCEEEEEEEECCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCCC
ALKAAQYTAEQVVNALGGHGIFGVELFVKGDHVIFNEVSPRPHDTGLVTLMSQDSSEFAL
CHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCCCCEEE
HVRAFTGMPIKSITQYGPCASAVILGQGTSTNIRFEGLTEALDAPQTQVRLFGKPDIDGR
EEEEECCCCHHHHHHCCCCCCEEEEECCCCCEEEECCHHHHHCCCCEEEEEECCCCCCCC
RRLGVTLTRRNSTETAIKDAIESAAKVKVIY
EEEEEEEEECCCCHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA