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The map label for this gene is ywbI [H]

Identifier: 218709671

GI number: 218709671

Start: 1807816

End: 1808688

Strand: Reverse

Name: ywbI [H]

Synonym: VS_1682

Alternate gene names: 218709671

Gene position: 1808688-1807816 (Counterclockwise)

Preceding gene: 218709674

Following gene: 218709669

Centisome position: 54.82

GC content: 43.87

Gene sequence:

>873_bases
ATGCTTGAATCAAAACCACTTCGACATTTTCTGGCTGTTGCGCAGTTTGGTAACTTCACCCAGGCCGCCAAAGCGCTGCA
TATCGCACAGCCTGCATTGAGTATTTCTATTAAAAAACTCGAACAGACGCTTGAGTTGATTCTCTTCCGTCGAGGAGATA
AATACGTAACATTAACGGAAGAGGGAAAAGTATTGTTCGAACACGCCAAGAGGATTGCTCAACAGTTCGATGATGCTCAA
CTCGCAATGAACGAGTTAAAGGGGTTGGAGAAAGGAGAAGTGCGGTTAGGCGCTCCAAGCATGATGGGCAGTTACTTTTT
CCCGCAAGTCGTGATGGCATTTAAAAGCCAATACCCAAACCTAAAGTTGACCTTGATTGATGCTGGCACCGCATCTATTC
GAGAAATGTTGTTAAACGGCGAACTCGATATTGGCGTAATCAACCATGAAAATGTGCCAGATGACCTTGAAACTGACCAC
CTGTTAAGCTCAGAAATGTTAGCTGTAGTTGGCAAAGATCACCCACTGGCAACGCAACCATCCATCACCTTTGAAGAGTT
TTTCGCTCAAGAACTGATTATGTTCAAGTCGGGTTATTTCCACCGTGATTTCATTGATGCGACCTGTGAAAAATACAATC
TCGATATGACCATCGCCTTCGAAACCAACTTATTGCCAATGATTCTTTCTATAGTAAAACGAGAGTTTGCCATTACTGCG
CTGCTGAGTTTGGTCACAGAATACGAAGAAGACGTTGTGGGGGTGCCATTTAAAGATCCTGTAAAACTCAATCTATCTTT
GGCTTGGAGAAAGGAAGGGTACTTGTCGAAGGCAGACCGAACCTTTATTGATTTCGTTAAGCGCTACGTGTGA

Upstream 100 bases:

>100_bases
CCTGCACGAGTTAGTAAGCTATTTGTGAACAAGTTATCAGTACAAGTGTAATTAAAGAAATATATTAAAAATATCACCTC
TATATATTTAATATATCGCC

Downstream 100 bases:

>100_bases
TTTAGAATGGTATAGAACAACAAAGCCCGAGTATCCTAGATTGGACATTCGGGCTTTATTATATAGAAGTATTACTGGCT
TAGAGCACTTAGGTACTCTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MLESKPLRHFLAVAQFGNFTQAAKALHIAQPALSISIKKLEQTLELILFRRGDKYVTLTEEGKVLFEHAKRIAQQFDDAQ
LAMNELKGLEKGEVRLGAPSMMGSYFFPQVVMAFKSQYPNLKLTLIDAGTASIREMLLNGELDIGVINHENVPDDLETDH
LLSSEMLAVVGKDHPLATQPSITFEEFFAQELIMFKSGYFHRDFIDATCEKYNLDMTIAFETNLLPMILSIVKREFAITA
LLSLVTEYEEDVVGVPFKDPVKLNLSLAWRKEGYLSKADRTFIDFVKRYV

Sequences:

>Translated_290_residues
MLESKPLRHFLAVAQFGNFTQAAKALHIAQPALSISIKKLEQTLELILFRRGDKYVTLTEEGKVLFEHAKRIAQQFDDAQ
LAMNELKGLEKGEVRLGAPSMMGSYFFPQVVMAFKSQYPNLKLTLIDAGTASIREMLLNGELDIGVINHENVPDDLETDH
LLSSEMLAVVGKDHPLATQPSITFEEFFAQELIMFKSGYFHRDFIDATCEKYNLDMTIAFETNLLPMILSIVKREFAITA
LLSLVTEYEEDVVGVPFKDPVKLNLSLAWRKEGYLSKADRTFIDFVKRYV
>Mature_290_residues
MLESKPLRHFLAVAQFGNFTQAAKALHIAQPALSISIKKLEQTLELILFRRGDKYVTLTEEGKVLFEHAKRIAQQFDDAQ
LAMNELKGLEKGEVRLGAPSMMGSYFFPQVVMAFKSQYPNLKLTLIDAGTASIREMLLNGELDIGVINHENVPDDLETDH
LLSSEMLAVVGKDHPLATQPSITFEEFFAQELIMFKSGYFHRDFIDATCEKYNLDMTIAFETNLLPMILSIVKREFAITA
LLSLVTEYEEDVVGVPFKDPVKLNLSLAWRKEGYLSKADRTFIDFVKRYV

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=281, Percent_Identity=29.1814946619217, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=29.2253521126761, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=29.2096219931272, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788297, Length=170, Percent_Identity=33.5294117647059, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=27.4914089347079, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=24.21875, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789204, Length=238, Percent_Identity=25.6302521008403, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=25.9649122807018, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=30.8988764044944, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI2367136, Length=237, Percent_Identity=27.8481012658228, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788748, Length=288, Percent_Identity=23.6111111111111, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081904, Length=206, Percent_Identity=30.0970873786408, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787530, Length=290, Percent_Identity=22.7586206896552, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788296, Length=198, Percent_Identity=29.2929292929293, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789506, Length=288, Percent_Identity=22.2222222222222, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082132, Length=143, Percent_Identity=31.4685314685315, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=127, Percent_Identity=30.7086614173228, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=119, Percent_Identity=33.6134453781513, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786508, Length=180, Percent_Identity=23.8888888888889, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788626, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786448, Length=161, Percent_Identity=31.055900621118, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=24.4897959183673, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32891; Mature: 32891

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESKPLRHFLAVAQFGNFTQAAKALHIAQPALSISIKKLEQTLELILFRRGDKYVTLTE
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
EGKVLFEHAKRIAQQFDDAQLAMNELKGLEKGEVRLGAPSMMGSYFFPQVVMAFKSQYPN
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCC
LKLTLIDAGTASIREMLLNGELDIGVINHENVPDDLETDHLLSSEMLAVVGKDHPLATQP
EEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SITFEEFFAQELIMFKSGYFHRDFIDATCEKYNLDMTIAFETNLLPMILSIVKREFAITA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LLSLVTEYEEDVVGVPFKDPVKLNLSLAWRKEGYLSKADRTFIDFVKRYV
HHHHHHHHHHCEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MLESKPLRHFLAVAQFGNFTQAAKALHIAQPALSISIKKLEQTLELILFRRGDKYVTLTE
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
EGKVLFEHAKRIAQQFDDAQLAMNELKGLEKGEVRLGAPSMMGSYFFPQVVMAFKSQYPN
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCC
LKLTLIDAGTASIREMLLNGELDIGVINHENVPDDLETDHLLSSEMLAVVGKDHPLATQP
EEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SITFEEFFAQELIMFKSGYFHRDFIDATCEKYNLDMTIAFETNLLPMILSIVKREFAITA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LLSLVTEYEEDVVGVPFKDPVKLNLSLAWRKEGYLSKADRTFIDFVKRYV
HHHHHHHHHHCEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]