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The map label for this gene is purA2 [H]

Identifier: 218709632

GI number: 218709632

Start: 1763565

End: 1764824

Strand: Direct

Name: purA2 [H]

Synonym: VS_1642

Alternate gene names: 218709632

Gene position: 1763565-1764824 (Clockwise)

Preceding gene: 218709629

Following gene: 218709645

Centisome position: 53.45

GC content: 48.33

Gene sequence:

>1260_bases
ATGCCATCTATTGTTGTTGTAGGCGCAAACTGGGGCGACGAAGGCAAAGGCCGTATCGTTGATTTTTTAGCAGACCAAGC
TTCTGCTAGCATTCGCTTTCAAGGCGGAAACAATGCTGGCCATACCGTAGTAAACGACTTCGGTACATTCAAACTGCATC
AACTGCCTAGTGGTATTTTTAATCCTGATTGTACGGCAGTTCTTGGCCCTGGCATGGTGATCAGCCCTGCTGCGCTAACT
CAAGAGATTGCAGAAGTACAAGCAGCTGGCATCAAAGTGAAAATGGCGATTTCAGACCGTGCGACACTTTGTCTACCTTT
ACACGCGCTTGAAGACACACTTGAAGAAGAACGTTTAGGCGATGGTGCTTACGGTTCAACACGTCAAGGTATTGCACCCG
CTTACGGCGATCGTGTGATGAAGAAAGGCATTCTTGTTGGCTGGTTGAATCAACCTGAAATTTTGGAGCAACGCATTCAA
TTCTTACTTGATTGGAAGATGCCTCAACTTAAAGCGCTATACCCACAATGTGACTTCTCTCAGACTGCTTCTGAAATGAC
AGAATGGTTACTTGAAGTGACTAAAGCTTGGCGCCCATTCATTTGCAACGTAACTGAGCCGCTTAAAGCACTGCAATCGC
AAGACGCGAACTTACTGTTCGAAGCTCAGTTAGGTGCAGGTCGCGACCTTGTTTACGGTGAATATCCTTGGACGACATCT
TCAAATGTAACCGCTGCATACGCAGGCATCGGTAGTGGTTTACCTGCCCTTCGCCCTGAGCGCGTTATTGCTGTGGCTAA
ATCGTTCAGCTCATCTGTAGGTACTGGTACGCTAGTGACTGCAATGGAAGAGCAAGATAGCTTCCGCGAAAGCTCTAACG
AATACGGCGCAACAACAGGCCGCCCACGTGATATGGGTTACTTCGACGCGGTAGCAACTCGCAACGGTGTTGACCTGCAA
GCTGCAACGGAAATCGCACTGACTAAGATTGATTGCCTGTCTGGTTTGTCTGAACTTAAGATCTGTACCGCTTACTCTGG
TGAGCACACTGAAAACCCGATTTGGCCACAAACGGCTGAACTAAAGCCTGTATATGAAGATATGGCTGGCTGGGATGAAG
ACATCACTGGCTGCCGCACGTTTGAGAGCCTTCCTCAAGCAGCACAAGATTACGTTACGCGTATCGAAGAGTTGATGGGC
GTGCCAATCGTGATGGTGTCAGTAGGTCCAGAGCGCGAACAAATGATCATTCTAGGTTAG

Upstream 100 bases:

>100_bases
CATGATATATGAAATCCTTATCAAGATAATAAAGATAATTAATTTCTCTTATCCACTTTGTAGGCCTAACATGATTTCTC
AATCAGTCAGGAGAAAAATT

Downstream 100 bases:

>100_bases
AGCTCTACTCAAGTATTTCAGCTAAAGCGGTTGTTTTGGCTGAAAGACGACAAACACAAAAAAGCCCACACTAGGTGGGC
TCTTTCGTATAATGGCGCTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPSIVVVGANWGDEGKGRIVDFLADQASASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPDCTAVLGPGMVISPAALT
QEIAEVQAAGIKVKMAISDRATLCLPLHALEDTLEEERLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEILEQRIQ
FLLDWKMPQLKALYPQCDFSQTASEMTEWLLEVTKAWRPFICNVTEPLKALQSQDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDSFRESSNEYGATTGRPRDMGYFDAVATRNGVDLQ
AATEIALTKIDCLSGLSELKICTAYSGEHTENPIWPQTAELKPVYEDMAGWDEDITGCRTFESLPQAAQDYVTRIEELMG
VPIVMVSVGPEREQMIILG

Sequences:

>Translated_419_residues
MPSIVVVGANWGDEGKGRIVDFLADQASASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPDCTAVLGPGMVISPAALT
QEIAEVQAAGIKVKMAISDRATLCLPLHALEDTLEEERLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEILEQRIQ
FLLDWKMPQLKALYPQCDFSQTASEMTEWLLEVTKAWRPFICNVTEPLKALQSQDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDSFRESSNEYGATTGRPRDMGYFDAVATRNGVDLQ
AATEIALTKIDCLSGLSELKICTAYSGEHTENPIWPQTAELKPVYEDMAGWDEDITGCRTFESLPQAAQDYVTRIEELMG
VPIVMVSVGPEREQMIILG
>Mature_418_residues
PSIVVVGANWGDEGKGRIVDFLADQASASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPDCTAVLGPGMVISPAALTQ
EIAEVQAAGIKVKMAISDRATLCLPLHALEDTLEEERLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEILEQRIQF
LLDWKMPQLKALYPQCDFSQTASEMTEWLLEVTKAWRPFICNVTEPLKALQSQDANLLFEAQLGAGRDLVYGEYPWTTSS
NVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDSFRESSNEYGATTGRPRDMGYFDAVATRNGVDLQA
ATEIALTKIDCLSGLSELKICTAYSGEHTENPIWPQTAELKPVYEDMAGWDEDITGCRTFESLPQAAQDYVTRIEELMGV
PIVMVSVGPEREQMIILG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=33.4106728538283, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=34.3457943925234, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=32.3308270676692, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=37.2365339578454, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=32.4825986078886, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=32.4825986078886, Blast_Score=221, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=31.0904872389791, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=34.1176470588235, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45480; Mature: 45349

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGANWGDEGKGRIVDFLADQASASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPDCTAVLGPGMVISPAALTQEIAEVQAAGIKVKMAISDRATLCLPLHALEDTLEEERLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEILEQRIQFLLDWKMPQLKALYPQCDFS
CCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
QTASEMTEWLLEVTKAWRPFICNVTEPLKALQSQDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDSFRESSNEYGATTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCC
RPRDMGYFDAVATRNGVDLQAATEIALTKIDCLSGLSELKICTAYSGEHTENPIWPQTAE
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCC
LKPVYEDMAGWDEDITGCRTFESLPQAAQDYVTRIEELMGVPIVMVSVGPEREQMIILG
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEC
>Mature Secondary Structure 
PSIVVVGANWGDEGKGRIVDFLADQASASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPDCTAVLGPGMVISPAALTQEIAEVQAAGIKVKMAISDRATLCLPLHALEDTLEEERLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEILEQRIQFLLDWKMPQLKALYPQCDFS
CCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
QTASEMTEWLLEVTKAWRPFICNVTEPLKALQSQDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDSFRESSNEYGATTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCC
RPRDMGYFDAVATRNGVDLQAATEIALTKIDCLSGLSELKICTAYSGEHTENPIWPQTAE
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCC
LKPVYEDMAGWDEDITGCRTFESLPQAAQDYVTRIEELMGVPIVMVSVGPEREQMIILG
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA