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The map label for this gene is sspA [H]

Identifier: 218709618

GI number: 218709618

Start: 1746521

End: 1747177

Strand: Reverse

Name: sspA [H]

Synonym: VS_1628

Alternate gene names: 218709618

Gene position: 1747177-1746521 (Counterclockwise)

Preceding gene: 218709619

Following gene: 218709617

Centisome position: 52.96

GC content: 41.55

Gene sequence:

>657_bases
ATGATTAAATTGATTAGCTTTAAAAACTGCCCATTCGTTCAACGTGTAATGGGTGCTTTAGTAATGAAAAATGTCCCGTT
TGAAATTGAGTACATTGAACTGAATAACAAACCACAGTGGTTTTTGGATATCTCACCGAACGGGCAGGTACCTGTATTAA
TTACAGAGAACGACACCGTCCTTTTTGAATCTGATGCAATTGTTGAATACCTAGACGATAAATATACGCCTATTGAAGAG
GTTTCACCCGAGCAGAAAGCACTCGACCGTGCTTGGTCTTATCAAGCGAGCAAACATTACATGCCACAGTGCGGCACCAT
GGCGAGTAAAGACAAAGAGACATTTGAAACACGCTTAGCCAATTTACAAAAGGCGTTCCTAAAGGCAGAGAAAAAACTGG
GTGATACTGAGTTCTTTAAAGGGGATTACATCTCTAACGTCGACATCGCTTGGTTACCACTGTTACACCGAGCATTTGTT
ATTAAAGAGCGTTCAGGCTTCAATATGCTAGAAAGCTTCCCGAAAGTACAGAAGTGGCAAGCGGCGCTGATTGAATCTGG
ATTGACCGATAAAACCGTTCCAGCGGACTTTATTGACAAGTTCAGCGGCTTTTATTTAACGAATAACTACTTGGCTAGTC
TCGCTGAAGTTCGTTAA

Upstream 100 bases:

>100_bases
TAAATTATTTCGTGAAATAACGAATTATTCTTCGTCTTTTGGATTTTGGGTTCGTCTTAGGGGGGTAACAAGAAACTATT
AACCCGATTCGGAGCCAAAA

Downstream 100 bases:

>100_bases
TAATATGAATTTAATTGAAATATATTTTAATGGCACGTTGCACTAAATAAATACCCTCCAAAAATAAAACTCACTCTCCT
GTTTATATCTTTGATTGGAT

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIKLISFKNCPFVQRVMGALVMKNVPFEIEYIELNNKPQWFLDISPNGQVPVLITENDTVLFESDAIVEYLDDKYTPIEE
VSPEQKALDRAWSYQASKHYMPQCGTMASKDKETFETRLANLQKAFLKAEKKLGDTEFFKGDYISNVDIAWLPLLHRAFV
IKERSGFNMLESFPKVQKWQAALIESGLTDKTVPADFIDKFSGFYLTNNYLASLAEVR

Sequences:

>Translated_218_residues
MIKLISFKNCPFVQRVMGALVMKNVPFEIEYIELNNKPQWFLDISPNGQVPVLITENDTVLFESDAIVEYLDDKYTPIEE
VSPEQKALDRAWSYQASKHYMPQCGTMASKDKETFETRLANLQKAFLKAEKKLGDTEFFKGDYISNVDIAWLPLLHRAFV
IKERSGFNMLESFPKVQKWQAALIESGLTDKTVPADFIDKFSGFYLTNNYLASLAEVR
>Mature_218_residues
MIKLISFKNCPFVQRVMGALVMKNVPFEIEYIELNNKPQWFLDISPNGQVPVLITENDTVLFESDAIVEYLDDKYTPIEE
VSPEQKALDRAWSYQASKHYMPQCGTMASKDKETFETRLANLQKAFLKAEKKLGDTEFFKGDYISNVDIAWLPLLHRAFV
IKERSGFNMLESFPKVQKWQAALIESGLTDKTVPADFIDKFSGFYLTNNYLASLAEVR

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=218, Percent_Identity=31.1926605504587, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI300360534, Length=191, Percent_Identity=31.9371727748691, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI38016131, Length=223, Percent_Identity=29.5964125560538, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI300360569, Length=214, Percent_Identity=29.9065420560748, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI300360532, Length=213, Percent_Identity=29.5774647887324, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789624, Length=148, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144449, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532652, Length=207, Percent_Identity=32.3671497584541, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532656, Length=207, Percent_Identity=32.3671497584541, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355779, Length=192, Percent_Identity=32.2916666666667, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355775, Length=187, Percent_Identity=30.4812834224599, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24660979, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355763, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355765, Length=213, Percent_Identity=27.2300469483568, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25056; Mature: 25056

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLISFKNCPFVQRVMGALVMKNVPFEIEYIELNNKPQWFLDISPNGQVPVLITENDTV
CEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCEEEEEECCCEE
LFESDAIVEYLDDKYTPIEEVSPEQKALDRAWSYQASKHYMPQCGTMASKDKETFETRLA
EEECCHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
NLQKAFLKAEKKLGDTEFFKGDYISNVDIAWLPLLHRAFVIKERSGFNMLESFPKVQKWQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHCHHHHHHH
AALIESGLTDKTVPADFIDKFSGFYLTNNYLASLAEVR
HHHHHCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHCC
>Mature Secondary Structure
MIKLISFKNCPFVQRVMGALVMKNVPFEIEYIELNNKPQWFLDISPNGQVPVLITENDTV
CEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCEEEEEECCCEE
LFESDAIVEYLDDKYTPIEEVSPEQKALDRAWSYQASKHYMPQCGTMASKDKETFETRLA
EEECCHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
NLQKAFLKAEKKLGDTEFFKGDYISNVDIAWLPLLHRAFVIKERSGFNMLESFPKVQKWQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHCHHHHHHH
AALIESGLTDKTVPADFIDKFSGFYLTNNYLASLAEVR
HHHHHCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]