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The map label for this gene is cry [H]

Identifier: 218709609

GI number: 218709609

Start: 1734815

End: 1736227

Strand: Direct

Name: cry [H]

Synonym: VS_1619

Alternate gene names: 218709609

Gene position: 1734815-1736227 (Clockwise)

Preceding gene: 218709608

Following gene: 218709610

Centisome position: 52.58

GC content: 43.81

Gene sequence:

>1413_bases
ATGAAAAAGATTGGTCTCTATCTTTTTACAAGCGACTTAAGAATCAACGACAATCAATTGCTCCATTGCGCAGCACAGAG
CGTCGACAAACTCATTTTCGTGATTGTCGAACCCACTTTGGTTCGTTTCTCTGCTGATCTCGCGCAAGAGCAAAGTTATG
GCGCGCATCGCCAGACTTTTGTTTCACAATCAATAGACAATTTAGAATTGAATTTGAATTTAGTGAGGCTTGGGCAGCAA
CTGGTTGTGATTCACAGTAATCATTTAGAACCAGACGCAGCCGAGCAAACACTCAGCCAAATCATTGCTACACAACACGT
CACCCACTTTTTCGCCAATGCACACTGCGGTTACGATGAGCGACAATTGATTCATTCACTACAAAGTCGTCACCCTGACT
TAATAACATGTTTGCCACACCACTCGACCTTGTTTGACTCACATGAGATGCCGTTTGAATTATCAAAAGTCCCGAGCTCG
TTTACCAAATTTAGAAAGCTAGTCGAACACTTGGACGTTAATGGCGGTGAGACGGTTATTGCACACCTTCCACCGCCAGT
GACACCAACATTGACACCAGCACACACAACGGCTATTCCAATATTCAGTTCTCCAAATGTAGAAAGAGCAGTAACAAACG
ATTACCTGGGTGGCGAAGACGCAGGACTAGCGCATTTAGATAACTACTTCGCACATGATTACGCCCTTAACTACAAGCAG
ACTCGAAACGCATTTGACGGCATCGAAAACTCAACCAAGTTTTCGCCGTGGTTGGCACTCGGTTGTGTTTCACCTAAAAC
GATTTGTCGCCATTTGAAACAGTTCGAAATCGAGCATGGCAGTAACGATTCAACCTATTGGATCTACTTTGAGTTGTTGT
GGCGCGAATATTTCTATTGGAAATGCTTATCTCAGGGTCCATCGTTATTCGGTAATTTTTCAGACAAAAAACTCTACCCC
CCAAACTCCTCGGGAACGTCGAACCTGAATTTCGCCAAATGGAAAAGTGGCAATACCAACTACCCGATTGTCGATGCATG
TATGCGCCAACTTAACGAAACAGGCTATATGTCTAATCGAGGAAGACAGCTTGCCGCTAGCTGTTTGATCTATGAACTAG
GGATCGATTGGCGACACGGTGCAGCCTATTTTGAGAGCCAACTTATCGACTATGACGTGGCATCAAACTGGGGTAATTGG
GCTTACATAGCCGGAGCCCTCAACTCTCAGGTAAACACCCAAGACAATAAACAAAATGATGCTAAGCAACCTAAACCTAA
AACCAAACCTAAATCACGACATTTTGATTTGGGTAAGCAAACGAATATGTACGATCCAGACCATGCGTTTATCAATAAGT
GGAATGCGAGCAGCAAAGAACCTGTCTTAACTAACAATCAGGATGTGTTATGA

Upstream 100 bases:

>100_bases
GCCCCAACCCAACTGTCACCCACTTGCTGAGTTCATTTGTGATCTTGTCAGCTTATCAACTCGTAAGCGTCTAATAATTA
ACAAATCACGAGTGTAAGTA

Downstream 100 bases:

>100_bases
ACTTCAAAACCGTGCGCCTTGTCTTAGGCGACCAGCTAAATATAGAGCACTCATGGTTTCGACAGGTTAACGATGACGTT
ATTTACATCATTGCTGAGCT

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKIGLYLFTSDLRINDNQLLHCAAQSVDKLIFVIVEPTLVRFSADLAQEQSYGAHRQTFVSQSIDNLELNLNLVRLGQQ
LVVIHSNHLEPDAAEQTLSQIIATQHVTHFFANAHCGYDERQLIHSLQSRHPDLITCLPHHSTLFDSHEMPFELSKVPSS
FTKFRKLVEHLDVNGGETVIAHLPPPVTPTLTPAHTTAIPIFSSPNVERAVTNDYLGGEDAGLAHLDNYFAHDYALNYKQ
TRNAFDGIENSTKFSPWLALGCVSPKTICRHLKQFEIEHGSNDSTYWIYFELLWREYFYWKCLSQGPSLFGNFSDKKLYP
PNSSGTSNLNFAKWKSGNTNYPIVDACMRQLNETGYMSNRGRQLAASCLIYELGIDWRHGAAYFESQLIDYDVASNWGNW
AYIAGALNSQVNTQDNKQNDAKQPKPKTKPKSRHFDLGKQTNMYDPDHAFINKWNASSKEPVLTNNQDVL

Sequences:

>Translated_470_residues
MKKIGLYLFTSDLRINDNQLLHCAAQSVDKLIFVIVEPTLVRFSADLAQEQSYGAHRQTFVSQSIDNLELNLNLVRLGQQ
LVVIHSNHLEPDAAEQTLSQIIATQHVTHFFANAHCGYDERQLIHSLQSRHPDLITCLPHHSTLFDSHEMPFELSKVPSS
FTKFRKLVEHLDVNGGETVIAHLPPPVTPTLTPAHTTAIPIFSSPNVERAVTNDYLGGEDAGLAHLDNYFAHDYALNYKQ
TRNAFDGIENSTKFSPWLALGCVSPKTICRHLKQFEIEHGSNDSTYWIYFELLWREYFYWKCLSQGPSLFGNFSDKKLYP
PNSSGTSNLNFAKWKSGNTNYPIVDACMRQLNETGYMSNRGRQLAASCLIYELGIDWRHGAAYFESQLIDYDVASNWGNW
AYIAGALNSQVNTQDNKQNDAKQPKPKTKPKSRHFDLGKQTNMYDPDHAFINKWNASSKEPVLTNNQDVL
>Mature_470_residues
MKKIGLYLFTSDLRINDNQLLHCAAQSVDKLIFVIVEPTLVRFSADLAQEQSYGAHRQTFVSQSIDNLELNLNLVRLGQQ
LVVIHSNHLEPDAAEQTLSQIIATQHVTHFFANAHCGYDERQLIHSLQSRHPDLITCLPHHSTLFDSHEMPFELSKVPSS
FTKFRKLVEHLDVNGGETVIAHLPPPVTPTLTPAHTTAIPIFSSPNVERAVTNDYLGGEDAGLAHLDNYFAHDYALNYKQ
TRNAFDGIENSTKFSPWLALGCVSPKTICRHLKQFEIEHGSNDSTYWIYFELLWREYFYWKCLSQGPSLFGNFSDKKLYP
PNSSGTSNLNFAKWKSGNTNYPIVDACMRQLNETGYMSNRGRQLAASCLIYELGIDWRHGAAYFESQLIDYDVASNWGNW
AYIAGALNSQVNTQDNKQNDAKQPKPKTKPKSRHFDLGKQTNMYDPDHAFINKWNASSKEPVLTNNQDVL

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=418, Percent_Identity=24.8803827751196, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI188536100, Length=416, Percent_Identity=23.5576923076923, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI188536103, Length=193, Percent_Identity=29.0155440414508, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1786926, Length=470, Percent_Identity=27.6595744680851, Blast_Score=131, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324962, Length=124, Percent_Identity=38.7096774193548, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137248, Length=454, Percent_Identity=23.7885462555066, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585455, Length=454, Percent_Identity=23.7885462555066, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648152, Length=430, Percent_Identity=22.3255813953488, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 53310; Mature: 53310

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGLYLFTSDLRINDNQLLHCAAQSVDKLIFVIVEPTLVRFSADLAQEQSYGAHRQTF
CCCEEEEEEEECEEECCCHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHH
VSQSIDNLELNLNLVRLGQQLVVIHSNHLEPDAAEQTLSQIIATQHVTHFFANAHCGYDE
HHHCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RQLIHSLQSRHPDLITCLPHHSTLFDSHEMPFELSKVPSSFTKFRKLVEHLDVNGGETVI
HHHHHHHHHCCCCEEEECCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEE
AHLPPPVTPTLTPAHTTAIPIFSSPNVERAVTNDYLGGEDAGLAHLDNYFAHDYALNYKQ
EECCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEECHHH
TRNAFDGIENSTKFSPWLALGCVSPKTICRHLKQFEIEHGSNDSTYWIYFELLWREYFYW
HHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
KCLSQGPSLFGNFSDKKLYPPNSSGTSNLNFAKWKSGNTNYPIVDACMRQLNETGYMSNR
HHHHCCCHHHCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
GRQLAASCLIYELGIDWRHGAAYFESQLIDYDVASNWGNWAYIAGALNSQVNTQDNKQND
CHHHHHHHHHHEECCCCCCCHHHHHHHHHEEHHHCCCCCEEEEEEECCCCCCCCCCCCCC
AKQPKPKTKPKSRHFDLGKQTNMYDPDHAFINKWNASSKEPVLTNNQDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIGLYLFTSDLRINDNQLLHCAAQSVDKLIFVIVEPTLVRFSADLAQEQSYGAHRQTF
CCCEEEEEEEECEEECCCHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHH
VSQSIDNLELNLNLVRLGQQLVVIHSNHLEPDAAEQTLSQIIATQHVTHFFANAHCGYDE
HHHCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RQLIHSLQSRHPDLITCLPHHSTLFDSHEMPFELSKVPSSFTKFRKLVEHLDVNGGETVI
HHHHHHHHHCCCCEEEECCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEE
AHLPPPVTPTLTPAHTTAIPIFSSPNVERAVTNDYLGGEDAGLAHLDNYFAHDYALNYKQ
EECCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEECHHH
TRNAFDGIENSTKFSPWLALGCVSPKTICRHLKQFEIEHGSNDSTYWIYFELLWREYFYW
HHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
KCLSQGPSLFGNFSDKKLYPPNSSGTSNLNFAKWKSGNTNYPIVDACMRQLNETGYMSNR
HHHHCCCHHHCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
GRQLAASCLIYELGIDWRHGAAYFESQLIDYDVASNWGNWAYIAGALNSQVNTQDNKQND
CHHHHHHHHHHEECCCCCCCHHHHHHHHHEEHHHCCCCCEEEEEEECCCCCCCCCCCCCC
AKQPKPKTKPKSRHFDLGKQTNMYDPDHAFINKWNASSKEPVLTNNQDVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]