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The map label for this gene is artI [H]

Identifier: 218709553

GI number: 218709553

Start: 1693685

End: 1694428

Strand: Reverse

Name: artI [H]

Synonym: VS_1563

Alternate gene names: 218709553

Gene position: 1694428-1693685 (Counterclockwise)

Preceding gene: 218709554

Following gene: 218709552

Centisome position: 51.36

GC content: 43.95

Gene sequence:

>744_bases
ATGAAAAAGATTCTACTAGCTTCACTTATCGGCCTTTCTTCTGCAGGCATGGCTGTTAATGCATCGGCACAAGACGAAAT
CAAATTCGCAATGGAAGCAACTTACGCACCATTCGAATACATGGACGAGAACAACCAAATCCAAGGTTTTGACGTAGACC
TAGCAAACGCACTTTGTGAAGAGATGAAAGCAACTTGTACTTTTCACAACCAAGCGTTCGATAGCTTGATCCCAGCGCTT
AAATTCAAACGTTACGATGCCGCTATTTCGGCAATGGACATCACTGAAGCGCGTCTTAAACAAGTAAACTTCTCTAACGC
TTACTACGACAACTCTGCAGCATTCATCTCTATTGAAGGCAAAGTAGCAGACCAAGCAGCACTAGAAGGTAAGCGTGTTG
GCGTTCAAAACGGTTCAACTCACCAGAGCTTCCTACTTGAGCAAATGGCTGGCGTAACAGCGGTACCTTACTCAAGCTAC
CAAGATGCATTCATCGACATGAAAAACGGTCGTATCGATTCAGTATTTGGTGACACAGCGGTAGTAGCTGAATGGTTCAA
AAAGCAAGACAACCTAACGTATGTTGGCGACCAAGTAACAAACCAAGAGTACTTCGGTAACGGCTTTGGTATCGCAGTAA
ACAAGAGTAACCAAGAACTTGTAGACCAGCTTAACGTTGCACTTGCAGCAGTGAAAGCGAACGGCGAATACGACAAGATC
TTTAACAAGTACTTCGGTAAGTAA

Upstream 100 bases:

>100_bases
GCTAATCACAACACAATTTAGTCAAACATAGATTCAGTTAAAACAAATCAAATTATAAAAATGGCCACAGGTCAAAATCA
CACAGATTCGGAGTAACAAA

Downstream 100 bases:

>100_bases
TTTATGGAATTAACGGGTTACTCTTTAGGGCTCGTCGAAGCAAGCTGGATGACAGTTCAGCTTGCGTTCGTAAGCCTATT
GGTAGGATTGGTTCTAGCAG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKKILLASLIGLSSAGMAVNASAQDEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCEEMKATCTFHNQAFDSLIPAL
KFKRYDAAISAMDITEARLKQVNFSNAYYDNSAAFISIEGKVADQAALEGKRVGVQNGSTHQSFLLEQMAGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKQDNLTYVGDQVTNQEYFGNGFGIAVNKSNQELVDQLNVALAAVKANGEYDKI
FNKYFGK

Sequences:

>Translated_247_residues
MKKILLASLIGLSSAGMAVNASAQDEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCEEMKATCTFHNQAFDSLIPAL
KFKRYDAAISAMDITEARLKQVNFSNAYYDNSAAFISIEGKVADQAALEGKRVGVQNGSTHQSFLLEQMAGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKQDNLTYVGDQVTNQEYFGNGFGIAVNKSNQELVDQLNVALAAVKANGEYDKI
FNKYFGK
>Mature_247_residues
MKKILLASLIGLSSAGMAVNASAQDEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCEEMKATCTFHNQAFDSLIPAL
KFKRYDAAISAMDITEARLKQVNFSNAYYDNSAAFISIEGKVADQAALEGKRVGVQNGSTHQSFLLEQMAGVTAVPYSSY
QDAFIDMKNGRIDSVFGDTAVVAEWFKKQDNLTYVGDQVTNQEYFGNGFGIAVNKSNQELVDQLNVALAAVKANGEYDKI
FNKYFGK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=57.085020242915, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=53.469387755102, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=35.4330708661417, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=35.8078602620087, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=29.8804780876494, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=27.8026905829596, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27161; Mature: 27161

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLASLIGLSSAGMAVNASAQDEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCE
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCHHHCCCCCCEEECCHHHHHHHHH
EMKATCTFHNQAFDSLIPALKFKRYDAAISAMDITEARLKQVNFSNAYYDNSAAFISIEG
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECC
KVADQAALEGKRVGVQNGSTHQSFLLEQMAGVTAVPYSSYQDAFIDMKNGRIDSVFGDTA
CCCHHHHHCCCEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEECCCCCCHH
VVAEWFKKQDNLTYVGDQVTNQEYFGNGFGIAVNKSNQELVDQLNVALAAVKANGEYDKI
HHHHHHHCCCCEEEECCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHEEEEECCCCHHHH
FNKYFGK
HHHHHCC
>Mature Secondary Structure
MKKILLASLIGLSSAGMAVNASAQDEIKFAMEATYAPFEYMDENNQIQGFDVDLANALCE
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCHHHCCCCCCEEECCHHHHHHHHH
EMKATCTFHNQAFDSLIPALKFKRYDAAISAMDITEARLKQVNFSNAYYDNSAAFISIEG
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECC
KVADQAALEGKRVGVQNGSTHQSFLLEQMAGVTAVPYSSYQDAFIDMKNGRIDSVFGDTA
CCCHHHHHCCCEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEECCCCCCHH
VVAEWFKKQDNLTYVGDQVTNQEYFGNGFGIAVNKSNQELVDQLNVALAAVKANGEYDKI
HHHHHHHCCCCEEEECCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHEEEEECCCCHHHH
FNKYFGK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]